Polymorphisms of the Endothelial Nitric Oxide Synthase Gene in Preeclampsia in a Han Chinese Population

Abstract

Background/Aims: The endothelial nitric oxide synthase (eNOS) gene has been enlisted by previous research as a candidate gene of preeclampsia predisposition. This study investigates the specific roles of 3 polymorphisms of the eNOS gene in a population of Chinese origin from mainland China. Methods: We studied the association of 3 commonly studied polymorphisms of the eNOS gene, namely 4b/a, T-786C and Glu298Asp, in a case-controlled sample of 220 patients diagnosed with preeclampsia and 200 healthy controls. The association between eNOS polymorphisms and preeclampsia was evaluated by performing genotyping for the eNOS variants and calculating odds ratios (OR) and 95% confidence intervals. The plasma nitrite concentration in participants was determined to examine how 3 eNOS polymorphisms affect plasma nitric oxide (NO) concentrations in pregnant women. Results: The frequencies of both the variant 298Asp allele and eNOS 4a allele were significantly lower in preeclamptic women than in the control group and had a significantly lower OR. The variant 298Asp allele and eNOS 4a are strongly associated with higher plasma NO concentrations in pregnant women. Conclusions: Polymorphisms in the eNOS gene may be protective against preeclampsia in a Chinese population, and this protective effect may be associated with NO formation in plasma in pregnant women.

Oxidative Stress and Dyslipidemia as Indicators of Pathogenesis of Preeclampsia in Pregnant Sudanese Women

“…Two variants 298Asp allele and eNOS 4a were strongly associated with higher plasma NO concentrations in pregnant women and suggested H. A. Hobiel, H. Y. Z. Tadros Journal of Biosciences and Medicines to modulate PE susceptibility [46]. Vascular tone is altered by the interference of ROS and NO, increased ROS production seems to suppress the expression of eNOS and hence reduced production of NO [47].…”

Associations between nitric oxide synthase 3 gene polymorphisms and preeclampsia risk: a meta-analysis

“…Not excluding studies with HWE-violation may result in the overestimation of the statistical significance of some postulated gene-disease associations, and it also seemed to modestly increase the between-study heterogeneity in some instances, thus distorting the findings 34 . In the current meta-analysis, seven studies for G894T 14 17 28 29 30 31 35 , two studies for T-786C 32 36 , and four studies for VNTR 4b/a 14 18 21 37 deviated from HWE. After adding these studies into the pooled analyses, the overall risk and between-study heterogeneity were not significantly changed except for a significant reduction of heterogeneity for G894T (from 52.5% to 38.8%).…”

“…In 2013, two meta-analyses on the associations between these three polymorphisms and preeclampsia risk were published but with inconsistent results 11 12 . Since the publication of these meta-analyses, eleven new studies have been published 13 14 15 16 17 18 19 20 21 22 23 . We have therefore performed an updated systematic review and meta-analysis, adding the recently published studies to further clarify the role of these three SNPs in susceptibility to preeclampsia and to address the limitations of the previous meta-analyses by using more sophisticated methods.…”