Polymorphonuclear Leukocytes Activation: In Vitro Modulation by 6-Methoxy-2-Naphthylacetic Acid, The Major Active Metabolite of Nabumetone

A, Allegrezza-Giulietti; Serretti, R.; Peroni, Mariana; Cervini, C

doi:10.1006/phrs.1993.1118

Cited by 3 publications

(2 citation statements)

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“…f‐MLP, phorbol ester and concanavalin A), we also tested the ability of NSAIDs to inhibit f‐MLP‐induced neutrophil reactive oxygen metabolites production 22 , . 30 , 31 Our data are consistent with previous studies which have shown that NSAIDs such as indomethacin, ibuprofen and phenylbutazone inhibit f‐MLP‐induced reactive oxygen metabolite production by human peripheral blood neutrophils 20 , . 22 , 23 , 30 The NSAIDs tested in this study did not act as reactive oxygen metabolite scavengers in the cell‐free superoxide assay, showing consistency with an earlier study 32…”

Section: Discussionsupporting

confidence: 91%

“…This suggests that, in the experiments described here, sulindac was acting via a cyclooxygenase‐independent mechanism. We did not measure conversion of nabumetone to 6‐MNA in our assay system but it has been previously demonstrated that conversion to 6‐MNA occurs readily in plasma and that 6‐MNA inhibits f‐MLP‐stimulated neutrophil reactive oxygen metabolites production in vitro 30 , . 41 Therefore, it is possible that the effects of nabumetone seen in our experiments were mediated by 6‐MNA.…”

Section: Discussionmentioning

confidence: 99%

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Non‐steroidal anti‐inflammatory drugs inhibit Helicobacter pylori‐induced human neutrophil reactive oxygen metabolite production in vitro

Jones-Blackett

et al. 1999

Aliment Pharmacol Ther

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Non‐steroidal anti‐inflammatory drugs inhibit Helicobacter pylori‐induced human neutrophil reactive oxygen metabolite production in vitro

Jones-Blackett

et al. 1999

Aliment Pharmacol Ther

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EFFECT OF THE HYDROXYL RADICAL ON FIBROBLAST‐MEDIATED COLLAGEN REMODELLING IN VITRO

Arisawa

Arisawa²,

Ohashi³

et al. 1996

Clin Exp Pharma Physio

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1. It has been reported that free radicals prevent wound healing. However, the mechanism of this effect is not yet clear. We attempted to clarify the influence of hydroxyl radicals on wound healing in vitro. 2. We used an ascorbate-copper ion system (ACS) to produce hydroxyl radicals in accordance with variables of time elapsed and concentration of copper ion. The effects of hydroxyl radical on fibroblast-mediated collagen remodelling, cell viability, the functions of fibroblasts and collagen fibrils were studied. 3. With a copper ion concentration of 100 mumol/L ACS significantly reduced contraction, while 10 mumol/L stimulated contraction. Hydrogen peroxide (H2O2) was employed in observing these findings. ACS did not influence cell viability, the expression of alpha 2 beta 1 integrin and cellular fibronectin, or the cytoskeletal organization of fibroblasts involving actin until 3 h. A concentration of ACS at 10 mumol/L of copper ion induced the polymerization of collagen after 30 min, while ACS at 100 mumol/L induced collagen degradation; this finding was also established by using H2O2. Collagen reduced the amount of formaldehyde produced by trapping hydroxyl radical with dimethyl sulfoxide. 4. Our findings suggest that collagen is denatured by scavenging the hydroxyl radical before fibroblasts are damaged, so that the radical may influence the remodelling of collagen.

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Non-steroidal anti-inflammatory drug, nabumetone, prevents indometacin-induced gastric damage via inhibition of neutrophil functions

Ishiwata

Okamoto

Yokochi

et al. 2003

Journal of Pharmacy and Pharmacology

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Selegiline has been formulated in an acrylic polymer adhesive mixture to be employed as a constant release topical patch for daily transdermal administration. Application of this selegiline transdermal system (STS) to guinea-pigs resulted in an average delivery of 1.185 mg selegiline/cm(2) patch/24 h. STS dose-response curves were generated by altering patch size (cm(2)). A transdermal dose range was identified which inhibited guinea-pig brain monoamine oxidase-B (MAO-B) by greater than 95% yet provided for a dose-dependent inhibition of monoamine oxidase-A (MAO-A) activity. The ID50 for inhibition of MAO-A activity in response to a 21-day daily regimen with transdermal selegiline was approximately 7.5-fold lower for cortical and striatal brain regions compared with that obtained for duodenum; hepatic MAO-A was unaffected following the same dosing regimen. By contrast, orally administered selegiline inhibited brain and duodenal MAO-A to the same extent, and generated a shallower dose-inhibition curve for brain MAO-A inhibition. In addition, transdermal delivery was approximately 6-8-times more potent than oral selegiline for the inhibition of brain MAO-A activity. It is concluded that daily transdermal selegiline administration may provide therapeutic advantages over oral treatment, based on its preferential, dose-dependent inhibition of brain vs peripheral MAO-A activity.

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Polymorphonuclear Leukocytes Activation: In Vitro Modulation by 6-Methoxy-2-Naphthylacetic Acid, The Major Active Metabolite of Nabumetone

Cited by 3 publications

References 0 publications

Non‐steroidal anti‐inflammatory drugs inhibit Helicobacter pylori‐induced human neutrophil reactive oxygen metabolite production in vitro

Non‐steroidal anti‐inflammatory drugs inhibit Helicobacter pylori‐induced human neutrophil reactive oxygen metabolite production in vitro

EFFECT OF THE HYDROXYL RADICAL ON FIBROBLAST‐MEDIATED COLLAGEN REMODELLING IN VITRO

Non-steroidal anti-inflammatory drug, nabumetone, prevents indometacin-induced gastric damage via inhibition of neutrophil functions

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