Polymyxin B broth disk elution: a feasible and accurate methodology to determine polymyxin B susceptibility in Enterobacterales

Cielo, Naiany C.; Belmonte, T.; Raro, Otávio Hallal Ferreira; Silva, Ravena M.C. da; Wink, Priscila Lamb; Barth, Afonso Luís; Cunha, Gabriela Rosa da; Mott, Mariana; Riche, Cezar Vinícius Würdig; Dias, Cícero; Caierão, Juliana

doi:10.1016/j.diagmicrobio.2020.115099

Cited by 9 publications

(7 citation statements)

References 33 publications

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“…But the poor diffusion of polymyxins in agar may affect the susceptibility results. Broth disk elution is another relatively cheap and easy-to-perform method, which has been tested for inferring polymyxin B susceptibility among Enterobacterales (Cielo et al 2020); however, the CLSI, the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the United States Committee on Antimicrobial Susceptibility Testing (USCAST) do not support paper diffusion-based and gradient diffusionbased methods at present. Prior to 2020, CLSI and EUCAST had not established breakpoints for polymyxin B in Enterobacterales (Turnidge et al 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Broth disk elution is another relatively cheap and easy‐to‐perform method, which has been tested for inferring polymyxin B susceptibility among Enterobacterales (Cielo et al . 2020); however, the CLSI, the European Committee on Antimicrobial Susceptibility Testing (EUCAST) and the United States Committee on Antimicrobial Susceptibility Testing (USCAST) do not support paper diffusion‐based and gradient diffusion‐based methods at present.…”

Section: Introductionmentioning

confidence: 99%

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Performance of different methods for testing polymyxin B: comparison of broth microdilution, agar dilution and MIC test strip in mcr‐1 positive and negative Escherichia coli

Wang¹,

Berglund

Zhu³

et al. 2021

Lett Appl Microbiol

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Significance and Impact of the Study: Antimicrobial susceptibility testing with the last-resort antibiotics polymyxins is associated with several methodological issues. The current reference method, broth microdilution (BMD), is laborious and the utility of alternative methods needs to be evaluated for polymyxin B susceptibility testing. Discordances in MIC test strips and agar dilution in comparison with BMD for polymyxin B susceptibility testing for Escherichia coli were observed, suggesting their inapplicability in routine testing.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Performance of different methods for testing polymyxin B: comparison of broth microdilution, agar dilution and MIC test strip in mcr‐1 positive and negative Escherichia coli

Wang¹,

Berglund

Zhu³

et al. 2021

Lett Appl Microbiol

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“…Cielo ve ark. (24) ise polimiksin B için SDE yöntemini referans metot ile karşılaştırmış ve KU'un %99.5, BH'nın %0, ÇBH'nın %1.11 olduğunu bildirmişlerdir. Fransa'da yapılan bir çalışmada, CRE izolatlarında kolistin direncini belirlemek için altı fenotipik yöntem ile SMD yöntemi karşılaştırılmış ve kolistin SDE yönteminin kategorik uyumunun %100 olduğu bildirilmiştir (25) .…”

Section: Discussionunclassified

Determination of Colistin Resistance in Gram Negative Bacteria by Modified Broth Disk Elution Method

Tüzemen¹,

Işık²,

Akça³

et al. 2022

Turk Mikrobiyol Cemiy Derg

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Amaç: Çalışmamız, kolistin direncini malzeme ve iş yükünden tasarruf ederek sıvı disk elüsyon (SDE) yönteminde kullanılan tüp ve kolistin diski sayısını azaltarak, iş yükü fazla olan tıbbi mikrobiyoloji laboratuvarlarının kullanabileceği alternatif bir yöntemi sunmak ve referans yöntem ile performansını karşılaştırmak amacıyla yapılmıştır. Yöntem: Hastanemizde 2020-2022 yılları arasında çeşitli klinik örneklerden izole edilen 100 adet otomatize sistem ile kolistin duyarlı, 50 adet kolistin dirençli Gram negatif bakteri çalışmaya alınmıştır. Tek tüp ile yapılan modifiye SDE yönteminin ve otomatize sistemin sonuçları, referans yöntem olan sıvı mikrodilüsyon (SMD) yöntemi ile kategorik uyum (KU),çok büyük hata (ÇBH) ve büyük hata (BH) oranları ile karşılaştırılmıştır. Bulgular: Otomatize sistemin tüm izolatlarda KU %86, ÇBH oranı %10.7 ve BH oranı %3.3 olarak bulunmuştur. Modifiye SDE yönteminin ise tüm izolatlarda KU %80, ÇBH oranı %4 ve BH oranı %16 olarak bulunmuştur. Otomatize sistemde ÇBH olarak yorumlanan 16 izolatın 10'u (%62.5) modifiye SDE yönteminde olması gerektiği gibi dirençli, BH olarak yorumlanan beş izolatın üçü (%60) ise modifiye SDE yönteminde olması gerektiği gibi duyarlı olarak saptanmıştır. Sonuç: Çoğu mikrobiyoloji laboratuvarında antibiyotik duyarlılık testinde kullanılan otomatize sistemin KU'mu ISO standartlarına yakın olmasına rağmen, kolistin duyarlılığı açısından ÇBH oranının çok fazla olduğu, bunların modifiye SDE yöntemi ile çalışıldığında bu oranın azaldığı saptanmıştır. Özellikle günlük antibiyogram sayısı fazla olan mikrobiyoloji laboratuvarlarında kolistin duyarlılık sonuçlarının SMD yöntemi ile çalışılamadığı durumlarda, Enterobacteriaceae ve Pseudomonas spp. izolatlarında otomatize sistemde kolistin duyarlı ise modifiye SDE yöntemi ile çalışılmasının daha güvenilir bir sonuç verdiği görülmüştür.

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“…In the present study, the disk elution method had 100% CA and 100% EA, with no errors at all (Figure 3). Disk elution was very easy to perform and interpret and it is also relatively affordable as compared to the other four methods [45,46].…”

Section: Discussionmentioning

confidence: 99%

Detection of colistin resistance via four methods in multidrug-resistant Gram-negative rods isolated from blood cultures

Alhamwi

Öksüz

2022

J Infect Dev Ctries

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Introduction: The broth microdilution (BMD) method recommended for the detection of colistin resistance is labor-intensive, time-consuming, and difficult to apply in routine laboratories. Thus, various methods, such as disk elution, commercial microdilution, and rapid polymyxin-NP tests have been developed for the detection of colistin resistance. In this study, a total of 102 multi-resistant Gram-negative bacteria isolated from blood cultures were evaluated by four different methods for the detection of colistin resistance, and compared with the reference method. Methodology: For the detection of the compatibility of these methods with the reference method, categorical and essential agreements, very major, major, and minor error rates were determined. Colistin-tigecycline and colistin-meropenem combinations were investigated in colistin-resistant isolates. Results: Of the isolates, 15 (15%) [K. pneumoniae (n = 12), A. baumannii (n = 2), E. coli (n = 1)] were resistant to colistin with reference BMD method. MIC50 and MIC90 values of all isolates were ≤ 0.25 μg/mL and 16 μg/mL, respectively. The categorical agreement rates were 100% for commercial microdilution, disk elution, and RPNP test. The essential agreement rates of commercial microdilution, disk elution, and broth macrodilution were 78.4%, 86.3%, and 100%, respectively. Although there were no major errors in these methods, the macrodilution (12%) and commercial microdilution (20.6%) methods showed the most minor errors. Colistin-meropenem combination showed a 100% synergistic effect, but the colistin-tigecycline combination showed an 80% synergistic effect and 20% indifference effect. Conclusions: Disk elution and RPNP tests are suitable for routine use because they are the most efficient, easiest, low-cost, and good performance tests in detecting colistin resistance.

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Polymyxin B broth disk elution: a feasible and accurate methodology to determine polymyxin B susceptibility in Enterobacterales

Cited by 9 publications

References 33 publications

Performance of different methods for testing polymyxin B: comparison of broth microdilution, agar dilution and MIC test strip in mcr‐1 positive and negative Escherichia coli

Performance of different methods for testing polymyxin B: comparison of broth microdilution, agar dilution and MIC test strip in mcr‐1 positive and negative Escherichia coli

Determination of Colistin Resistance in Gram Negative Bacteria by Modified Broth Disk Elution Method

Detection of colistin resistance via four methods in multidrug-resistant Gram-negative rods isolated from blood cultures

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