Polypeptide Self-Assembled Nanoparticles as Delivery Systems for Polymyxins B and E

Iudin, Dmitrii; Zashikhina, Natalia; Demyanova, Elena; Korzhikov-Vlakh, Viktor; Shcherbakova, Elena S.; Boroznjak, Roman; Tarasenko, I. I.; Захарова, Н. В.; Lavrentieva, Antonina; Skorik, Yury А.; Korzhikova-Vlakh, Evgenia

doi:10.3390/pharmaceutics12090868

Cited by 26 publications

(38 citation statements)

References 50 publications

(66 reference statements)

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“…The high biocompatibility for polymers of glutamic acid with cells was earlier demonstrated in several papers [ 38 , 39 , 51 ]. Taking into account the high biocompatibility of PCL and low content of the filler in composites, the absence of cytotoxicity for composite films could be expected.…”

Section: Resultsmentioning

confidence: 91%

Aminated Graphene-Graft-Oligo(Glutamic Acid) /Poly(ε-Caprolactone) Composites: Preparation, Characterization and Biological Evaluation

et al. 2021

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Biodegradable and biocompatible composites are of great interest as biomedical materials for various regeneration processes such as the regeneration of bones, cartilage and soft tissues. Modification of the filler surface can improve its compatibility with the polymer matrix, and, as a result, the characteristics and properties of composite materials. This work is devoted to the synthesis and modification of aminated graphene with oligomers of glutamic acid and their use for the preparation of composite materials based on poly(ε-caprolactone). Ring-opening polymerization of N-carboxyanhydride of glutamic acid γ-benzyl ester was used to graft oligomers of glutamic acid from the surface of aminated graphene. The success of the modification was confirmed by Fourier-transform infrared and X-ray photoelectron spectroscopy as well as thermogravimetric analysis. In addition, the dispersions of neat and modified aminated graphene were analyzed by dynamic and electrophoretic light scattering to monitor changes in the characteristics due to modification. The poly(ε-caprolactone) films filled with neat and modified aminated graphene were manufactured and carefully characterized for their mechanical and biological properties. Grafting of glutamic acid oligomers from the surface of aminated graphene improved the distribution of the filler in the polymer matrix that, in turn, positively affected the mechanical properties of composite materials in comparison to ones containing the unmodified filler. Moreover, the modification improved the biocompatibility of the filler with human MG-63 osteoblast-like cells.

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Section: Resultsmentioning

confidence: 91%

Aminated Graphene-Graft-Oligo(Glutamic Acid) /Poly(ε-Caprolactone) Composites: Preparation, Characterization and Biological Evaluation

et al. 2021

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“…As it was mentioned earlier, the trapping systems for the application in vivo should be quite small, stable, and have a reduced uptake by macrophages. It is known, that PEG- b -PLA nanoparticles have a good stability due to additional surface hydrophilization and lower uptake by microphages than PLA nanoparticles [ 37 ]. Moreover, PEGylation is also well-known approach to reduce non-specific interaction with plasma proteins [ 38 ].…”

Section: Resultsmentioning

confidence: 99%

Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus

et al. 2021

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Hepatitis C is one of the most common social diseases in the world. The improvements in both the early diagnostics of the hepatitis C and the treatment of acute viremia caused by hepatitis C virus are undoubtedly an urgent task. In present work, we offered the micro- and nanotraps for the capturing of HCV. As a capturing moiety, we designed and synthesized in E. coli a fusion protein consisting of large extracellular loop of CD81 receptor and streptavidin as spacing part. The obtained protein has been immobilized on the surface of PLA-based micro- and nanoparticles. The developed trapping systems were characterized in terms of their physico-chemical properties. In order to illustrate the ability of developed micro- and nanotraps to bind HCV, E2 core protein of HCV was synthesized as a fusion protein with GFP. Interaction of E2 protein and hepatitis C virus-mimicking particles with the developed trapping systems were testified by several methods.

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“…CT sulfate (MW of 1390) was procured from BetaPharm (Wujiang, Shanghai, China). The contents of CT A (polymyxin E1) and CT B (polymyxin E2) were 31.1 ± 0.4% and 68.9 ± 0.4%, respectively [49].…”

Section: Methodsmentioning

confidence: 95%

Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carriers for Improved Colistin Delivery

Dubashynskaya

Raik

Dubrovskii

et al. 2021

IJMS

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Improving the therapeutic characteristics of antibiotics is an effective strategy for controlling the growth of multidrug-resistant Gram-negative microorganisms. The purpose of this study was to develop a colistin (CT) delivery system based on hyaluronic acid (HA) and the water-soluble cationic chitosan derivative, diethylaminoethyl chitosan (DEAECS). The CT delivery system was a polyelectrolyte complex (PEC) obtained by interpolymeric interactions between the HA polyanion and the DEAECS polycation, with simultaneous inclusion of positively charged CT molecules into the resulting complex. The developed PEC had a hydrodynamic diameter of 210–250 nm and a negative surface charge (ζ-potential = −19 mV); the encapsulation and loading efficiencies were 100 and 16.7%, respectively. The developed CT delivery systems were characterized by modified release (30–40% and 85–90% of CT released in 15 and 60 min, respectively) compared to pure CT (100% CT released in 15 min). In vitro experiments showed that the encapsulation of CT in polysaccharide carriers did not reduce its antimicrobial activity, as the minimum inhibitory concentrations against Pseudomonas aeruginosa of both encapsulated CT and pure CT were 1 μg/mL.

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Polypeptide Self-Assembled Nanoparticles as Delivery Systems for Polymyxins B and E

Cited by 26 publications

References 50 publications

Aminated Graphene-Graft-Oligo(Glutamic Acid) /Poly(ε-Caprolactone) Composites: Preparation, Characterization and Biological Evaluation

Aminated Graphene-Graft-Oligo(Glutamic Acid) /Poly(ε-Caprolactone) Composites: Preparation, Characterization and Biological Evaluation

Polymer Particles Bearing Recombinant LEL CD81 as Trapping Systems for Hepatitis C Virus

Hyaluronan/Diethylaminoethyl Chitosan Polyelectrolyte Complexes as Carriers for Improved Colistin Delivery

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