Polypeptide sequence of the chlorophyll a/b/c-binding protein of the prasinophycean alga Mantoniella squamata

Schmitt, Anne-Marie; Frank, Gerhard; James, Peter; Staudenmann, Werner; Zuber, Herbert; Wilhelm, Christian

doi:10.1007/bf00034776

Cited by 20 publications

(8 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The alignment of amino acid sequences reveals a phylogenetic relationship of this apoprotein to Chl ah-binding polypeptides (29). One has to remember that in accordance with recent evidence, P. marinus and P. didemni contain a Chl c-like pigment together with (divinyl) Chls a and b (7,15).…”

supporting

confidence: 62%

Another View on the Role of Photosynthetic Pigments in Taxonomy of Oxygenic-Phototrophic Bacteria: Proposed Rejection of the Order Prochlorales Florenzano, Balloni, and Materassi 1986 (Emend. Burger-Wiersma, Stal, and Mur 1989), the Family Prochloraceae Florenzano, Balloni, and Materassi 1986, and the Family Prochlorotrichaceae Burger-Wiersma, Stal, and Mur 1989

Pinevich

Averina

Величко

1997

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

We propose that the order Prochlorales Florenzano, Balloni, and Materassi 1986 (emend. Burger-Wiersma, Stal, and Mur 1989), the family Prochloraceae Florenzano, Balloni, and Materassi 1986, and the family Prochlorotrichaceae Burger-Wiersma, Stal, and Mur 1989, validly published in the International Journal of Systematic Bacteriology under the rules of the Bacteriological Code, be rejected because of the imperfection of ordinal diagnosis. The oxygenic-phototrophic prokaryotes involved are proposed to be incorporated under their validly published names into the orders Chroococcales and Oscillatoriales of the "Cyanobacteria" group. Correspondingly, the latter is proposed to be upgraded to equal "Oxygenic photosynthetic bacteria" (Section 19 in Bergey's Manual).Oxygenic phototrophy is the generation of proton motive force due to light-driven transport of electrons which are extracted from water; a universal complement of the genomes in oxygenic phototrophs are the psa, psb, and wox gene assemblages (12). Oxygenic phototrophs are assigned to 1 of 11 major bacterial phyla, which has been convincingly demonstrated by the analysis of 16s rRNA sequences (22). The diversity of oxygenic phototrophs comprises, beside the members of Section 19, "Oxygenic photosynthetic bacteria" (6), bacterium-derived cellular entities: cyanelles and plastids. The former are obligate endocytobionts in the glaucophyceans, whereas the latter are prokaryote partners-with eukaryotes as second partner-in the photosynthetic chimerae, which belong to the kingdoms Plantae, Chromista, and Protozoa (8).Cyanobacteria are considered to be the principal group of oxygenic-phototrophic bacteria because of a broad spectrum of revealed objects and deep penetration into their structure and functions. A characteristic feature of cyanobacteria is the phycobilisome, the major light-harvesting complex (LHC) built of phycobiliprotein heterodimers which are interconnected with colorless polypeptide linkers. Phycobilisomes are anchored to the protoplasmic surface of the light energy-transducing membrane with a bilin chromophore-bearing polypeptide (1). Besides the cyanobacteria, oxygenic-phototrophic bacteria include a small assemblage of prochlorophytes which is hallmarked by a possession of the intramembranous, carotenoid chlorophyll (Chl) a/b-containing LHC instead of the "traditional" p hycobilisome.Oxygenic tributed to the present knowledge about oxygenic-phototrophic phylogeny that includes the "prochloralian" genera (33) show that 16s rRNA sequencing results do not support phenotype-based classification schemes. This conflict is not uncommon in prokaryotes (34), and since taxonomy operates with organisms rather than with genomes, the priority is conventionally given to essentials of the metabolism and/or cell architecture (21). Unfortunately, a classification based on physiological distinctions, which proves a success in major bacterial groupings, is unattainable in oxygenic-phototrophic bacteria because of a high level of uniformity of their metabolism. As a...

show abstract

supporting

confidence: 62%

Another View on the Role of Photosynthetic Pigments in Taxonomy of Oxygenic-Phototrophic Bacteria: Proposed Rejection of the Order Prochlorales Florenzano, Balloni, and Materassi 1986 (Emend. Burger-Wiersma, Stal, and Mur 1989), the Family Prochloraceae Florenzano, Balloni, and Materassi 1986, and the Family Prochlorotrichaceae Burger-Wiersma, Stal, and Mur 1989

Pinevich

Averina

Величко

1997

International Journal of Systematic Bacteriology

View full text Add to dashboard Cite

show abstract

“…The SCP MLHC and DM MLHC showed the typical LHC apoproteins of M. squamata with MWs of 23.5 and 25 kDa (Fig. 2D, Schmitt et al 1994). The SCP MLHC was purer and no additional polypeptide bands were found in the SDS-PAGE gels.…”

Section: Polypeptide Compositionmentioning

confidence: 96%

Influence of thylakoid membrane lipids on the structure of aggregated light‐harvesting complexes of the diatom Thalassiosira pseudonana and the green alga Mantoniella squamata

Schaller-Laudel

Latowski

Jemioła-Rzemińska

et al. 2017

Physiologia Plantarum

Self Cite

View full text Add to dashboard Cite

The study investigated the effect of the thylakoid membrane lipids monogalactosyldiacylglycerol (MGDG), digalactosyldiacylglycerol (DGDG), sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG) on the structure of two algal light-harvesting complexes (LHCs). In contrast to higher plants whose thylakoid membranes are characterized by an enrichment of the neutral galactolipids MGDG and DGDG, both the green alga Mantoniella squamata and the centric diatom Thalassiosira pseudonana contain membranes with a high content of the negatively charged lipids SQDG and PG. The algal thylakoids do not show the typical grana-stroma differentiation of higher plants but a regular arrangement. To analyze the effect of the membrane lipids, the fucoxanthin chlorophyll protein (FCP) complex of T. pseudonana and the LHC of M. squamata (MLHC) were prepared by successive cation precipitation using Triton X-100 as detergent. With this method, it is possible to isolate LHCs with a reduced amount of associated lipids in an aggregated state. The results from 77 K fluorescence and photon correlation spectroscopy show that neither the neutral galactolipids nor the negatively charged lipids are able to significantly alter the aggregation state of the FCP or the MLHC. This is in contrast to higher plants where SQDG and PG lead to a strong disaggregation of the LHCII whereas MGDG and DGDG induce the formation of large macroaggregates. The results indicate that LHCs which are integrated into thylakoid membranes with a high amount of negatively charged lipids and a regular arrangement are less sensitive to lipid-induced structural alterations than their counterparts in membranes enriched in neutral lipids with a grana-stroma differentiation.

show abstract

“…Whereas most of these algae do not contain Chl b , some Prasinophycean algae, such as Montoniella squamata , accumulate a Chl c , 3,8-divinyl-Pchlide, along with Chls a and b (Schmitt et al. 1994 ; Green and Durnford 1996 ). The red alga that contributed its plastid in the secondary endosymbiotic event apparently lacked Chl c .…”

Section: Summary Of Differences In Chlorophyllsmentioning

confidence: 99%

“…The Chl-binding proteins in these organisms are evolutionarily related to those in green algae and plants (Schmitt et al. 1994 ; Green and Durnford 1996 ). The relatively simple LHCs are well-defined structures and consequently are excellent systems to search for basic mechanisms.…”

Section: Introductionmentioning

confidence: 99%

Chlorophylls, ligands and assembly of light-harvesting complexes in chloroplasts

2007

View full text Add to dashboard Cite

Chlorophyll (Chl) b serves an essential function in accumulation of light-harvesting complexes (LHCs) in plants. In this article, this role of Chl b is explored by considering the properties of Chls and the ligands with which they interact in the complexes. The overall properties of the Chls, not only their spectral features, are altered as consequences of chemical modifications on the periphery of the molecules. Important modifications are introduction of oxygen atoms at specific locations and reduction or desaturation of sidechains. These modifications influence formation of coordination bonds by which the central Mg atom, the Lewis acid, of Chl molecules interacts with amino acid sidechains, as the Lewis base, in proteins. Chl a is a versatile Lewis acid and interacts principally with imidazole groups but also with sidechain amides and water. The 7-formyl group on Chl b withdraws electron density toward the periphery of the molecule and consequently the positive Mg is less shielded by the molecular electron cloud than in Chl a. Chl b thus tends to form electrostatic bonds with Lewis bases with a fixed dipole, such as water and, in particular, peptide backbone carbonyl groups. The coordination bonds are enhanced by H-bonds between the protein and the 7-formyl group. These additional strong interactions with Chl b are necessary to achieve assembly of stable LHCs.

show abstract

Polypeptide sequence of the chlorophyll a/b/c-binding protein of the prasinophycean alga Mantoniella squamata

Cited by 20 publications

References 35 publications

Influence of thylakoid membrane lipids on the structure of aggregated light‐harvesting complexes of the diatom Thalassiosira pseudonana and the green alga Mantoniella squamata

Chlorophylls, ligands and assembly of light-harvesting complexes in chloroplasts

Contact Info

Product

Resources

About