Polypeptides IGF-1C and P24 synergistically promote osteogenic differentiation of bone marrow mesenchymal stem cells in vitro through the p38 and JNK signaling pathways

Ran, Gaoying; Fang, Wei; Zhang, Lifang; Peng, Yung-Hsing; Wu, Anbiao; Li, Jiatong; Ding, Xiaoxia; Zeng, Su; He, Yan

doi:10.1016/j.biocel.2021.106091

Cited by 6 publications

(4 citation statements)

References 38 publications

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“…Previous studies suggested that the mitogen‐activated protein kinase (MAPK)‐dependent activation plays a role in the osteogenesis of MSCs (Ran et al, 2021; Zhu et al, 2018) and is involved in various biological effects of HG environment on cells in vitro (Pan et al, 2022; Shan et al, 2019). Therefore, we examined whether the MAPK pathway mediates the Ca‐phytate effect of HG‐treated hBMSCs by facilitating osteogenic differentiation.…”

Section: Resultsmentioning

confidence: 99%

“…JNK, ERK and p38 are the three MAPK pathways that have been identified. To date, there have been several reports on the involvement of the MAPK signalling pathway in the osteogenic differentiation of MSCs (Ran et al, 2021; Zhu et al, 2018). Therefore, we speculated whether the MAPK signalling pathway is involved in Ca‐phytate‐enhanced osteogenic differentiation of hBMSCs exposed to HG.…”

Section: Discussionmentioning

confidence: 99%

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Calcium phytate reverses high glucose‐inhibited osteogenesis of BMSCs via the MAPK/JNK pathway

Wang

Liu

et al. 2023

Oral Diseases

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ObjectivesDiabetes mellitus (DM) induces oxidative tissue impairment and suppresses bone formation. Some studies have shown that phytic acid has antioxidant and anti‐diabetic properties. This study aimed to investigate the potential of calcium phytate (Ca‐phytate) to reverse inhibited osteogenesis of human bone marrow mesenchymal stem cells (hBMSCs) in a high glucose (HG) environment and to determine the underlying mechanism.Materials and MethodshBMSCs were exposed to HG and palmitic acid to simulate DM in vitro. Osteogenic differentiation was measured using alkaline phosphatase staining and activity assay, alizarin red S staining, qRT‐PCR, Western blot and immunofluorescence staining. A critical‐size cranial defect model of type 2 diabetes mellitus (T2DM) rats was established to evaluate bone regeneration. A specific pathway inhibitor was used to explore whether the MAPK/JNK pathway was involved.ResultsTreatment with 34 μM Ca‐phytate had the highest effect on osteogenic differentiation in HG. Ca‐phytate improved cranial bone defect healing in T2DM rats. The long‐term HG environment inhibited the activation of the MAPK/JNK signalling pathway, which was restored by Ca‐phytate. Blocking the JNK pathway reduced the Ca‐phytate‐mediated osteogenic differentiation of hBMSCs.ConclusionCa‐phytate induced bone regeneration in vivo and reversed HG‐inhibited osteogenesis of hBMSCs in vitro via the MAPK/JNK signalling pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Calcium phytate reverses high glucose‐inhibited osteogenesis of BMSCs via the MAPK/JNK pathway

Wang

Liu

et al. 2023

Oral Diseases

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“…During the development from predentin to secondary dentin, regulation of the p38 MAPK pathway is essential in the secretion activity of odontoblasts [46,47]. It was also reported that the expressions of ALP and OCN were significantly reduced after inhibiting the p38 MAPK signaling pathway [48].…”

Section: Discussionmentioning

confidence: 98%

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling

Mu¹,

Chen²,

Bi³

et al. 2022

Int. J. Med. Sci.

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Tissue regeneration is the preferred treatment for dentin and bone tissue defects. Dental pulp stem cells (DPSCs) have been extensively studied for their use in tissue regeneration, including the regeneration of dentin and bone tissue. LIM mineralization protein-1 (LMP-1) is an intracellular non-secretory protein that plays a positive regulatory role in the mineralization process. In this study, an LMP-1-induced DPSCs model was used to explore the effect of LMP-1 on the proliferation and odonto/osteogenic differentiation of DPSCs, as well as the underlying mechanisms. As indicated by the cell counting kit-8 assay, the results showed that LMP-1 did not affect the proliferation of DPSCs. Overexpression of LMP-1 significantly promoted the committed differentiation of DPSCs and vice versa, as shown by alkaline phosphatase activity assay, alizarin red staining, western blot assay, quantitative real-time polymerase chain reaction assay, and in vivo mineralized tissue formation assay. Furthermore, inhibiting the activation of the extracellular signal-regulated kinase 1/2 (ERK1/2), p38 mitogen-activated protein kinase (MAPK), and c-Jun N-terminal kinase (JNK) pathways using specific pathway inhibitors showed that the ERK1/2 and p38 MAPK pathways attenuated the differentiation of DPSCs. Besides, the expression of BMP signaling pathway components were also determined, which suggested that LMP-1 could activate BMP-2/Smad1/5 signaling pathway. Our results not only indicated the underlying mechanism of LMP-1 treated DPSCs but also provided valuable insight into therapeutic strategies in regenerative medicine.

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“…Western blotting assays were performed as previously described. 73 The antibodies used are listed in key resources table . Nuclear protein samples were separated from whole cell lysates using the NE-PER kit ( Figure S20 ).…”

Section: Methodsmentioning

confidence: 99%

The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis

Wu,

Zhong,

Song

et al. 2024

iScience

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Polypeptides IGF-1C and P24 synergistically promote osteogenic differentiation of bone marrow mesenchymal stem cells in vitro through the p38 and JNK signaling pathways

Cited by 6 publications

References 38 publications

Calcium phytate reverses high glucose‐inhibited osteogenesis of BMSCs via the MAPK/JNK pathway

Calcium phytate reverses high glucose‐inhibited osteogenesis of BMSCs via the MAPK/JNK pathway

LIM Mineralization Protein-1 Enhances the Committed Differentiation of Dental Pulp Stem Cells through the ERK1/2 and p38 MAPK Pathways and BMP Signaling

The activation of LBH-CRYAB signaling promotes cardiac protection against I/R injury by inhibiting apoptosis and ferroptosis

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