Polyphasic characterization of xanthomonads pathogenic to members of the Anacardiaceae and their relatedness to species of Xanthomonas

Ah-You, Nathalie; Gagnevin, Lionel; Grimont, Patrick A. D.; Brisse, Sylvain; Nesme, Xavier; Chiroleu, Frédéric; Ngoc, Lan Bui Thi; Jouen, Emmanuel; Lefeuvre, Pierre; Vernière, Christian; Pruvost, Olivier

doi:10.1099/ijs.0.65453-0

Cited by 83 publications

(83 citation statements)

References 58 publications

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“…Isolates of X. euvesicatoria from Australian capsicum and chilli crops were genetically similar to isolates from overseas based on phylogenetic analysis of the atpD, efp, dnaK, and gyrB genes (Ah-You et al 2009;Hamza et al 2010Hamza et al , 2012. Australian isolates of X. euvesicatoria were positive in the X. euvesicatoria species-specific PCR, supporting the grouping of these isolates in the MLSA phylogeny.…”

Section: Discussionmentioning

confidence: 86%

“…PCRs were performed as described in Koenraadt et al (2009) using 2.5 U of MangoTaq™ (Bioline) and 1 μl of template. Primers and their annealing temperatures are listed in Table 2. MLSA-PCR primers targeting the chaperone protein dnaK (dnaK), elongation factor P (efp), ATP synthase subunit beta (atpD) and DNA gyrase subunit B (gyrB) genes (Table 2) were used as described (Boudon et al 2005;Ah-You et al 2009;Ngoc et al 2010;Hamza et al 2012). Annealing temperatures were optimised for the MLSA primers targeting all four genes.…”

Section: Biochemical Analysis and Pathogenicitymentioning

confidence: 99%

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Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia

Roach

Mann

Gambley³

et al. 2017

Eur J Plant Pathol

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Several species of Xanthomonas cause bacterial leaf spot, a disease that affects solanaceous crops worldwide. The diversity of 64 Australian isolates of Xanthomonas spp. associated with bacterial leaf spot in tomato, capsicum and chilli crops in eastern Australia was determined using multi-locus sequence analysis of atpD, dnaK, efp and gyrB genes, species-specific PCR assays and biochemical analyses. At least five species of Xanthomonas associated with bacterial leaf spot were identified in Australian tomato, capsicum and chilli crops and their pathogenicity assessed. Phylogenetic and biochemical analyses identified X. euvesicatoria, X. perforans and X. vesicatoria as the most frequently recovered pathogenic species. Non-pathogenic and weakly pathogenic species were also identified. The suitability of the identification methods used and the implications of the detection of these species will be discussed.Keywords amylolytic . pectolytic . Solanaceae . disease management IntroductionBacterial leaf spot (BLS) is a disease of solanaceous crops that occurs worldwide, especially in warm and humid climates (Jones et al. 2014). Several species of Xanthomonas are reported to cause BLS of tomato (Solanum lycopersicum), capsicum and chilli (Capsicum annuum) . The symptoms of BLS are small, brown, angular, water-soaked lesions on leaves, stems and fruit, and result in defoliation and direct fruit damage. Severe infection may result in extensive damage to crops with significant yield losses (Pernezny et al. 2003). Species reported to cause BLS all produce similar symptoms on their hosts, making precise diagnosis difficult from visual symptoms alone. The impact of pathological convergence and importance of phylogenetic testing in the case of BLS are further highlighted by Hajri et al. (2009). The causal bacteria are spread by wind and water, and may survive in crop residues, weeds and volunteer plants (Jones et al. 1986). A link between field disease and seed contamination has Eur J Plant Pathol (2018) 150:595-608

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Section: Discussionmentioning

confidence: 86%

Section: Biochemical Analysis and Pathogenicitymentioning

confidence: 99%

Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia

Roach

Mann

Gambley³

et al. 2017

Eur J Plant Pathol

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“…citri (15), and back again to X. citri subsp. citri (16)(17)(18). This highlights the great disconnect between taxonomy, pathology, ecology, and phylogeny of a complex group of bacteria like Xanthomonas.…”

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Genomic Insights into the Evolutionary Origin of Xanthomonas axonopodis pv. citri and Its Ecological Relatives

Midha

Patil

2014

Appl Environ Microbiol

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Xanthomonas axonopodis pv. citri (Xac) is the causal agent of citrus bacterial canker (CBC) and is a serious problem worldwide. Like CBC, several important diseases in other fruits, such as mango, pomegranate, and grape, are also caused by Xanthomonas pathovars that display remarkable specificity toward their hosts. While citrus and mango diseases were documented more than 100 years ago, the pomegranate and grape diseases have been known only since the 1950s and 1970s, respectively. Interestingly, diseases caused by all these pathovars were noted first in India. Our genome-based phylogenetic studies suggest that these diverse pathogens belong to a single species and these pathovars may be just a group of rapidly evolving strains. Furthermore, the recently reported pathovars, such as those infecting grape and pomegranate, form independent clonal lineages, while the citrus and mango pathovars that have been known for a long time form one clonal lineage. Such an understanding of their phylogenomic relationship has further allowed us to understand major and unique variations in the lineages that give rise to these pathovars. Whole-genome sequencing studies including ecological relatives from their putative country of origin has allowed us to understand the evolutionary history of Xac and other pathovars that infect fruits.

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“…Pathovars were defined as groups of strains sharing several pathological characteristics, such as their host range and the disease facies they cause (18). Based on molecular data, strains classified as a single pathovar usually form a discrete monomorphic or weakly polymorphic cluster, suggesting that strains of a pathovar have a common ancestral origin (3,56). Xanthomonas citri pv.…”

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From Local Surveys to Global Surveillance: Three High-Throughput Genotyping Methods for Epidemiological Monitoring of Xanthomonas citri pv. citri Pathotypes

Ngoc

Vernière

Jarne

et al. 2009

Appl Environ Microbiol

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Asiatic citrus canker is a major disease worldwide, and its causal agent, Xanthomonas citri pv. citri, is listed as a quarantine organism in many countries. Analysis of the molecular epidemiology of this bacterium is hindered by a lack of molecular typing techniques suitable for surveillance and outbreak investigation. We report a comparative evaluation of three typing techniques, amplified fragment length polymorphism (AFLP) analysis, insertion sequence ligation-mediated PCR (IS-LM-PCR) typing, and multilocus variable-number tandem-repeat analysis (MLVA), with 234 strains originating from Asia, the likely center of origin of the pathogen, and reference strains of pathotypes A, A*, and A w , which differ in host range. The typing techniques were congruent in describing the diversity of this strain collection, suggesting that the evolution pattern of the bacterium may be clonal. Based on a hierarchical analysis of molecular variance, the AFLP method best described the genetic variation found among pathotypes whereas MLVA best described the variation found among individual strains from the same countries or groups of neighboring countries. IS-LM-PCR data suggested that the transposition of insertion sequences in the genome of X. citri pv. citri occurs rarely enough not to disturb the phylogenetic signal. This technique may be useful for the global surveillance of nonepidemiologically related strains. Although pathological characteristics of strains could be most often predicted from genotyping data, we report the occurrence in the Indian peninsula of strains genetically related to pathotype A* strains but with a host range similar to that of pathotype A, which makes the classification of this bacterium even more complicated.

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Polyphasic characterization of xanthomonads pathogenic to members of the Anacardiaceae and their relatedness to species of Xanthomonas

Cited by 83 publications

References 58 publications

Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia

Identification of Xanthomonas species associated with bacterial leaf spot of tomato, capsicum and chilli crops in eastern Australia

Genomic Insights into the Evolutionary Origin of Xanthomonas axonopodis pv. citri and Its Ecological Relatives

From Local Surveys to Global Surveillance: Three High-Throughput Genotyping Methods for Epidemiological Monitoring of Xanthomonas citri pv. citri Pathotypes

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