Polyphasic emended description of the filamentous prochlorophyte Prochlorothrix scandica Skulberg 2008

Величко, Н. В.; Timofeyeva, Anna S.; Gavrilova, O. V.; Averina, Svetlana; Ivanikova, Natalia V.; Pinevich, Alexander V.

doi:10.1127/1864-1318/2012/0044

Cited by 5 publications

(6 citation statements)

References 19 publications

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“…This is particularly true for cyanobacteria, a group that has undergone extensive taxonomic restructuring in recent years, with the integration of molecular, cytomorphological, and ecophysiological approaches (Komárek et al, 2014). The closest relationship of our taxon to the genus Prochlorothrix is interesting, since the two described species (P. hollandica and P. scandica) are clearly freshwater filamentous species that lack phycobiliproteins (Burger-Wiersma et al, 1989;Pinevich et al, 1999;Velichko et al, 2013). The ability to form filaments may vary even within a species so further study of ultrastructural features, such as the arrangement of thylakoids, are needed to shed light onto the true relationship among these taxa.…”

Section: Species-level Tools For Understanding Bloom Dynamicsmentioning

confidence: 90%

High-Resolution Spatiotemporal Dynamics of Harmful Algae in the Indian River Lagoon (Florida)—A Case Study of Aureoumbra lagunensis, Pyrodinium bahamense, and Pseudo-nitzschia

et al. 2021

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The Indian River Lagoon (IRL), located on the east coast of Florida, is a complex estuarine ecosystem that is negatively affected by recurring harmful algal blooms (HABs) from distinct taxonomic/functional groups. Enhanced monitoring was established to facilitate rapid quantification of three recurrent bloom taxa, Aureoumbra lagunensis, Pyrodinium bahamense, and Pseudo-nitzschia spp., and included corroborating techniques to improve the identification of small-celled nanoplankton (<10 μm in diameter). Identification and enumeration of these target taxa were conducted during 2015–2020 using a combination of light microscopy and species-specific approaches, specifically immunofluorescence flow cytometry as well as a newly developed qPCR assay for A. lagunensis presented here for the first time. An annual bloom index (ABI) was established for each taxon based on occurrence and abundance data. Blooms of A. lagunensis (>2 × 108 cells L–1) were observed in all 6 years sampled and across multiple seasons. In contrast, abundance of P. bahamense, largely driven by the annual temperature cycle that moderates life cycle transitions and growth, displayed a strong seasonal pattern with blooms (105–107 cells L–1) generally developing in early summer and subsiding in autumn. However, P. bahamense bloom development was delayed and abundance was significantly lower in years and locations with sustained A. lagunensis blooms. Pseudo-nitzschia spp. were broadly distributed with sporadic bloom concentrations (reaching 107 cells L–1), but with minimal concentrations of the toxin domoic acid detected (<0.02 μg L–1). In summer 2020, multiple monitoring tools characterized a novel nano-cyanobacterium bloom (reaching 109 cells L–1) that coincided with a decline in A. lagunensis and persisted into autumn. Statistical and time-series analyses of this spatiotemporally intensive dataset highlight prominent patterns in variability for some taxa, but also identify challenges of characterizing mechanisms underlying more episodic yet persistent events. Nevertheless, the intersect of temperature and salinity as environmental proxies proved to be informative in delineating niche partitioning, not only in the case of taxa with long-standing data sets but also for seemingly unprecedented blooms of novel nanoplanktonic taxa.

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Section: Species-level Tools For Understanding Bloom Dynamicsmentioning

confidence: 90%

High-Resolution Spatiotemporal Dynamics of Harmful Algae in the Indian River Lagoon (Florida)—A Case Study of Aureoumbra lagunensis, Pyrodinium bahamense, and Pseudo-nitzschia

et al. 2021

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“…Samples for TEM were prepared as in Velichko et al. (2012) and viewed at an accelerating voltage of 50–70 kV in a JEM‐1400 electron microscope (Jeol Ltd, Tokyo, Japan) equipped with an Olympus‐SIS Veleta camera. Samples for SEM were prepared by fixation in 4.0% glutaraldehyde in 0.1 M Na‐phosphate buffer (pH 7.0), placed onto 1.0 μm‐mesh Millipore membrane filters (Sigma, Burlington, MA, USA), post‐fixed with 1.0% osmium tetroxide in distilled water, dehydrated in graded ethanol and acetone series, critical‐point‐dried in a Leica EM CPD 300 apparatus, shadowed with ca.…”

Section: Methodsmentioning

confidence: 99%

“…For the Altericista strains, fatty acids were extracted, converted to methyl‐ethers (FAMEs), and analyzed as described in Velichko et al. (2012); margaric acid (Sigma) served as a control. Individual FAMEs were identified and quantified using the NIST and Wiley search libraries, and the ChemStation software (Agilent Technologies, Inc., Santa Clara, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

A new cyanobacterial genus Altericista and three species, A. lacusladogae sp. nov., A. violacea sp. nov., and A. variichlora sp. nov., described using a polyphasic approach

Averina

Polyakova

Senatskaya

et al. 2021

Journal of Phycology

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Several strains of unicellular cyanobacteria from the culture collection of St. Petersburg State University, St. Petersburg, Russia (CALU), which were preliminary identified as Synechocystis sp., are reclassified in the new genus Altericista. Three new species are proposed, A. lacusladogae, A. violacea, and A. variichlora. The last species produces accessory chlorophylls d and f in cultures illuminated by far‐red light, an attribute rarely observed in cyanobacteria, especially in unicellular strains. This genus is morphologically similar to Synechocysis having coccoid cells that divide in two successive planes at right angles, containing no sheath or capsule, and having the lamellar system represented by peripheral concentric thylakoids. Altericista shows ecological, biochemical, and physiological characters unlike those in Synechocystis and has the distinguishing phenotypic characters as follows: freshwater, non‐halotolerant ecotype; palmitate and α‐linoleate as major fatty acids; and the ability to photoacclimate, including several types of complementary chromatic adaptation. Genetic differences from Synechocystis sp. include 16S rRNA, rpoC1, and rbcL gene sequences, as well as sequence and folding of 16S‐23S ITS.

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“…Possession of accessory b-type Chls is the specific trait for a combined group of PBS-lacking cyanobacteria (Goericke and Repeta 1992, van der Staay et al 1998. These are represented by unicellular species Prochloron didemni (Lewin 1975) and Prochlorococcus marinus (Chisholm et al 1988), and filamentous species Prochlorothrix hollandica (Burger-Wiersma et al 1989) and P. scandica (Velichko et al 2012) not closely related to one another. The unicellular species Acaryochloris thomasi RCC1774 is similar to these cyanobacteria in that it contains Chl b (at the Chls a/b molecular ratio of 6.25), and differs from them in that it contains a PBS (see below).…”

Section: B-type Chlorophylls: Participation In Light-harvesting Antenmentioning

confidence: 99%

Non-a chlorophylls in cyanobacteria

Averina¹,

Величко²,

Пиневич³

et al. 2019

Photosynt.

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In cyanobacteria and chloroplasts, chlorophyll a (Chl a) is not always the single type of Chl used in oxygenic photosynthesis. Rather, there is a series of non-a Chls, namely, b-type Chls, c-type Chls, Chl d, and Chl f. Plenty of reviews published over the past decades commented on these Chls in chloroplasts while only few analogously dealt with cyanobacteria. The review article takes an effort to span the gap. Cyanobacterial b-type and c-type Chls are exclusively antenna pigments; they absorb near-red and blue light, respectively, and facilitate waste-less energy input to reaction centers. Chl d and possibly Chl f partake in both antennae and reaction centers; they empower constitutive usage of far-red light or participate in the adaptive mechanism of far-red light photoacclimation.

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Polyphasic emended description of the filamentous prochlorophyte Prochlorothrix scandica Skulberg 2008

Cited by 5 publications

References 19 publications

High-Resolution Spatiotemporal Dynamics of Harmful Algae in the Indian River Lagoon (Florida)—A Case Study of Aureoumbra lagunensis, Pyrodinium bahamense, and Pseudo-nitzschia

High-Resolution Spatiotemporal Dynamics of Harmful Algae in the Indian River Lagoon (Florida)—A Case Study of Aureoumbra lagunensis, Pyrodinium bahamense, and Pseudo-nitzschia

A new cyanobacterial genus Altericista and three species, A. lacusladogae sp. nov., A. violacea sp. nov., and A. variichlora sp. nov., described using a polyphasic approach

Non-a chlorophylls in cyanobacteria

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