Polyphasic studies of new species of Diaporthe from native forest in Chile, with descriptions of Diaporthe araucanorum sp. nov., Diaporthe foikelawen sp. nov. and Diaporthe patagonica sp. nov.

Abstract

<jats:p>During a survey of fungi in native forests in Chile, several unidentified isolates of <jats:italic>Diaporthe</jats:italic> were collected from different hosts. The isolates were characterized based on DNA comparisons, morphology, culture characteristics and host affiliation, in accordance with previous descriptions. Phylogenetic analysis of the ITS region, combined with partial <jats:italic>tub2</jats:italic> and <jats:italic>tef1</jats:italic> genes, showed that the isolates formed three distinct groups representing three new taxa. The three new species of <jats:italic>Diaporthe</jats:italic>, <jats:italic>Diaporthe araucanorum</jats:italic> on <jats:italic>Araucaria araucana</jats:italic>, <jats:italic>Diaporthe foikelawen</jats:italic> on <jats:italic>Drimys winteri</jats:italic> and <jats:italic>Diaporthe patagonica</jats:italic> on <jats:italic>Aristotelia chilensis</jats:italic> are described and illustrated in the present study.</jats:p>

Biocontrol Potential and Catabolic Profile of Endophytic Diaporthe eres Strain 1420S from Prunus domestica L. in Poland—A Preliminary Study

“…Our research describes for the first time the endophytic D. eres, whose occurrence on P. domestica in Poland has been previously reported [14] but its characterization has not been performed before. The identification of the strain was confirmed based on morphological features and sequence data analysis of ITS, TEF, TUB, and CAL genes in accordance with other scientists' research where the delimitation of species within the genus Diaporthe only proved satisfactory once morphology combined with multi-gene DNA sequence data were analyzed [13,15,[17][18][19][20][21][22][23][24][25][26][27][28][29].…”

“…For many years, their identification has been based on morphological features and host-plant associations [12]. Recently, multi-locus DNA data analysis combined with morphological characterization and host associations has been employed for species delimitation in the genus [13,15,[17][18][19][20][21][22][23][24][25][26][27][28][29]. Although several endophytic Diaporthe species have been described as endophytes on woody plants worldwide [3,20,[30][31][32][33][34][35], there is no available information concerning endophytic Diaporthe on Prunus domestica in Poland or worldwide.…”

Molecular Characterization of Diaporthe Species Associated With Hazelnut Defects

“…Therefore, species and species complex limits need to be carefully defined, and for that reason, no species-level classifications were performed at this point. Recent studies have successfully identified new species based on the same three loci used here (ITS, EF1-α, and tub) (Lesuthu et al, 2019;Zapata et al, 2020). Nevertheless, some studies used four or more loci (Udayanga et al, 2014a(Udayanga et al, ,b, 2015Zhou and Hou, 2019), and a study from Santos et al (2017a) showed that the most optimal Diaporthe species separation occurred when five loci were simultaneously used.…”

Morphological and phylogenetic analyses reveal three new species of Diaporthe from Yunnan, China

“…Currently, more than 1100 epithets of Diaporthe are listed in Index Fungorum (http://www.indexfungorum.org/; accessed 1 Nov. 2020), but only one-fifth of these taxa have been well-studied with ex-type cultures and supplementary DNA barcodes (Guo et al 2020;Yang et al 2020;Zapata et al 2020). Species of Diaporthe are widely distributed and have a broad range of hosts including economically significant agricultural crops and ornamental plants such as species of Camellia, Castanea, Citrus, Glycine, Helianthus, Juglans, Persea, Pyrus, Vaccinium, Vitis and many more (van Rensburg et al 2006;Santos and Phillips 2009;Crous et al 2011aCrous et al , b, 2016Santos et al 2011;Thompson et al 2011;Grasso et al 2012;Huang et al 2013;Lombard et al 2014;Gao et al 2015Gao et al , 2016Gao et al , 2017Udayanga et al 2012Udayanga et al , 2015Guarnaccia et al 2016;Dissanayake et al 2017;Guarnaccia and Crous 2017;Fan et al 2018;Senanayake et al 2018;Guo et al 2020).…”

“…Phylogenetic analysis using a five-locus dataset (ITS-TUB-TEF-CAL-HIS) has been determined to be the optimal combination to identify species of Diaporthe species, as revealed by Santos et al (2017). Many Diaporthe species are described based on a polyphasic approach together with morphological characterisation (Rehner and Uecker 1994; Udayanga et al 2011;Gao et al 2017;Guarnaccia and Crous 2017;Yang et al 2018aYang et al , 2020Crous et al 2020;Dayarathne et al 2020;Guo et al 2020;Hyde et al 2020;Li et al 2020;Zapata et al 2020).…”