2019
DOI: 10.1002/cbdv.201900314
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Polyphenol Characterization, Antioxidant and Skin Whitening Properties of Alnus cordata Stem Bark

Abstract: In this study, we investigated the phenolic composition of the crude extract (MeOH 80 %) of Alnus cordata (Loisel.) Duby stem bark (ACE) and its antioxidant and skin whitening properties. RP‐LC‐DAD analysis showed a high content of hydroxycinnamic acids (47.64 %), flavanones (26.74 %) and diarylheptanoids (17.69 %). Furthermore, ACE exhibited a dose‐dependent antioxidant and free‐radical scavenging activity, expressed as half‐maximal inhibitory concentration (IC50): Oxygen radical absorbance capacity (ORAC, IC… Show more

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Cited by 19 publications
(18 citation statements)
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“…Antioxidant and skin whitening properties were demonstrated in Alnus cordata by Smeriglio et al, in 2019 [ 97 ]. Analyzing the phenolic composition, a significant presence of flavanones (26.74%), such as epicatechin, orientin, isovitexin, naringenin and eriodictyol was shown, therefore confirming that zebrafish is a useful model for the in vivo analysis of anti-melanogenesis agents.…”
Section: Effects Of Flavonoids Employing Zebrafish As Experimental Modelmentioning
confidence: 99%
“…Antioxidant and skin whitening properties were demonstrated in Alnus cordata by Smeriglio et al, in 2019 [ 97 ]. Analyzing the phenolic composition, a significant presence of flavanones (26.74%), such as epicatechin, orientin, isovitexin, naringenin and eriodictyol was shown, therefore confirming that zebrafish is a useful model for the in vivo analysis of anti-melanogenesis agents.…”
Section: Effects Of Flavonoids Employing Zebrafish As Experimental Modelmentioning
confidence: 99%
“…[16] with minor modifications. [24] Briefly, aliquots (0.05 mL) of sample at various concentrations (2-80 μM) were mixed with 0.5 mL of l-tyrosine or l-DOPA solution (1.25 mM), 0.9 mL of sodium acetate buffer solution (0.05 M, pH 6.8) and preincubated at 25°C for 10 min. Then 0.05 mL of an aqueous solution of mushroom tyrosinase (333 U/mL) was added last to the mixture.…”
Section: Mushroom Tyrosinase Inhibition Assaymentioning
confidence: 99%
“…The anti-tryptic activity was evaluated according to Smeriglio et al (2020). In brief, 200 μl of the test sample (7.81-62.50 μg/ml and 15.625-125 μg/ml for PEs and FEs, respectively; 31.25-250 μg/ml and 125-1,000 μg/ml for betanin and indicaxanthin, respectively) was added to the reaction mixture consisting of 12 μl trypsin (10 μg/ ml) and 188 μl Tris-HCl buffer (25 mM, pH 7.5).…”
Section: Protease Inhibition Assaymentioning
confidence: 99%