Polyphenol components of the knotwood extracts of Salix capreal.

Tsvetkov, D. E.; Dmitrenok, A. S.; Tsvetkov, Yury E.; Chizhov, Alexander O.; Nifantiev, Nikolay E.

doi:10.1007/s11172-020-3050-x

Cited by 6 publications

(2 citation statements)

References 25 publications

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“…ESI-MS detected twelve ion peaks from 20-HM. Three of the peaks were assigned as background or contaminant peaks [ 27 ], and the remaining peaks (nine peaks) were proposed as a main fragmentation of 20-HM as presented in Figure 1 ; Figure 2 by combining a search through literature and chemical databases and a prediction from CFM-ID version 3.0 software [ 17 , 28 , 29 ]. Most of the proposed fragments were in a range of ±5 mDa from a theoretical mass calculated by ACD/Chemsketch (free version, 2015).…”

Section: Resultsmentioning

confidence: 99%

In Vitro Production and Exudation of 20-Hydroxymaytenin from Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. Cell Culture

Pitakbut

Spiteller

Kayser

2021

Plants

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The metabolite 20-Hydroxymaytenin (20-HM) is a member of the quinone-methide pentacyclic triterpenoids (QMTs) group. This metabolite group is present only in Celastraceae plants, and it has shown various biological activities from antioxidant to anticancer properties. However, most QMTs metabolites including 20-HM cannot be synthesized in a laboratory. Therefore, we optimized a plant tissue culture protocol and examined the potential of Gymnosporia heterophylla (synonym. Maytenus heterophylla) to produce 20-HM in an in vitro experiment. For the first time, we reported the optimum callus induction medium with a high percentage success rate of 82% from the combination of 1 mg/L indole-3-butyric acid and 5 mg/L naphthalene acetic acid. Later, our cell suspension culture cultivated in the optimum medium provided approximately 0.35 mg/g fresh weight of 20-HM. This concentration is roughly 87.5 times higher than a concentration of 20-HM presenting in Elaeodendron croceum (Celastraceae) leaves. In addition, we also found that 20-HM presented in a cultivation medium, suggesting that G. heterophylla cells secreted 20-HM as an exudate in our experiment. Noticeably, 20-HM was missing when Penicillium cf. olsonii occurred in the medium. These findings hint at an antifungal property of 20-HM.

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Section: Resultsmentioning

confidence: 99%

In Vitro Production and Exudation of 20-Hydroxymaytenin from Gymnosporia heterophylla (Eckl. and Zeyh.) Loes. Cell Culture

Pitakbut

Spiteller

Kayser

2021

Plants

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“…3 was purified by reversed-phase HPLC (Cosmosil 5C 18 -MS-Ⅱ, 20 φ × 250 mm) with 50% MeOH–1% acetic acid to obtain astragalin ( 9 , 31.0 mg, 0.0247%), rutin ( 10 , 10.0 mg, 0.0080%), and quercetin 3- O -β-cellobioside ( 11 , 14.6 mg, 0.0112%). The chemical structures of the isolated compounds were identified by a comparison of the 1 H- and 13 C- NMR spectra with References [ 2 , 19 , 20 , 54 , 55 , 56 , 57 , 58 , 59 , 60 ]. The chemical structures of the isolated compounds ( 1 – 11 ) are shown in Figure 1 .…”

Section: Methodsmentioning

confidence: 99%

Lycoperoside H, a Tomato Seed Saponin, Improves Epidermal Dehydration by Increasing Ceramide in the Stratum Corneum and Steroidal Anti-Inflammatory Effect

et al. 2021

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Tomatoes are widely consumed, however, studies on tomato seeds are limited. In this study, we isolated 11 compounds including saponins and flavonol glycosides from tomato seeds and evaluated their effects on epidermal hydration. Among the isolated compounds, tomato seed saponins (10 µM) significantly increased the mRNA expression of proteins related to epidermal hydration, including filaggrin, involucrin, and enzymes for ceramide synthesis, by 1.32- to 1.91-fold compared with the control in HaCaT cells. Tomato seed saponins (10 µM) also decreased transepidermal water loss by 7 to 13 g/m2·h in the reconstructed human epidermal keratinization (RHEK) models. Quantitative analysis of the ceramide content in the stratum corneum (SC) revealed that lycoperoside H (1–10 µM) is a promising candidate to stimulate ceramide synthesis via the upregulation of ceramide synthase-3, glucosylceramide synthase, and β-glucocerebrosidase, which led to an increase in the total SC ceramides (approximately 1.5-fold) in concert with ceramide (NP) (approximately 2-fold) in the RHEK models. Evaluation of the anti-inflammatory and anti-allergic effects of lycoperoside H demonstrated that lycoperoside H is suggested to act as a partial agonist of the glucocorticoid receptor and exhibits anti-inflammatory effects (10 mg/kg in animal test). These findings indicate that lycoperoside H can improve epidermal dehydration and suppress inflammation by increasing SC ceramide and steroidal anti-inflammatory activity.

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