Polyphenol content and antioxidant activities of Prunus padus L. and Prunus serotina L. leaves: Electrochemical and spectrophotometric approach and their antimicrobial properties

Abstract

The aim of the study was to compare the content of selected phytochemicals as well as the antioxidant and antimicrobial potential of the leaves of Prunus padus L. and Prunus serotina L., as there is very little research on this subject in the literature. Therefore, it is used to deepen knowledge on this subject. In addition, an electrochemical test was also carried out, which was not yet available for the above plants. Antibacterial studies have also been deepened to include the analysis of new strains of bacteria and fungi, which has not been studied earlier. The water extracts of P. padus using the utra-performance liquid chromatography (UPLC) system showed a higher content of both phenolic acids and flavonols (651.77b ± 18.12 mg/100 g dw for acids and 3.85b ± 0.08 mg/100 g dw for flavonols, respectively). Ferulic and p-coumaric acids were the dominant polyphenols in leaves. Extracts from P. padus showed higher activity against DPPH radical, which was 6.62b ± 0.06 mg TE/1 g dw, as well as higher antioxidant capacity, measured using 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) cation radical (37.39b ± 3.81 mg TE/g dw). The higher antioxidant potential of P. padus was confirmed based on the oxidizing potentials of electroactive compounds present in them. Stronger inhibition against Enterococcus faecium and Klebsiella pneumoniae was found for P. padus, whereas P. serotina extract was more potent against Enterococcus faecium bacterium. It has been shown that P. padus can be an attractive raw material with antioxidant and antimicrobial properties that can be used on a much wider scale in food technology than its current application.

Phenolic composition of leaf and flower extracts of black cherry (Prunus serotina Ehrh.)

“…There is still a lack of information on the influence of process methods on phenolics extraction from different plant parts of P. serotina. In previous studies (Olszewska 2005a(Olszewska , b, 2007Techlichowska et al 2020), phenolics compounds in Prunus serotina have also been reported, but, in our study, we performed a detailed analysis and accurate identification of a broad spectrum of phenolics in three different extracts.…”

“…The main sources of polyphenolics in the European diet are beverages: tea and coffee (Clifford 1999;Scalbert and Williamson 2000;Zamora-Ros et al 2015). The leaves, flowers and bark of black cherry contain bioactive substances (Olszewska 2005a(Olszewska , b, 2007Techlichowska et al 2020). Extracts of these plant parts are used in traditional Mexican medicine.…”

“…Furthermore, differences in chemical composition between black cherry and other forest understory species may suggest P. serotina as a species of first choice in term of phenolic extractives. There have been only a few studies on phenolics from European populations of black cherry (Olszewska 2005a(Olszewska , b, 2007Techlichowska et al 2020). It follows from interviews with foresters that, for now, black cherry utilization is limited to firewood or for preparing infusions or liqueurs.…”

Exploring antimicrobial and antioxidant properties of phytocomponents from different anatomical parts of Prunus padus L

“…The research material consisted of leaves, bark, and fruits of P. padus that had been selected in previous studies as a raw material with a higher antioxidant potential [6] of leaves comparing with P. serrotina. [6] All anatomical parts of the P. padus were in the orchard farm in Ozierany Małe in Podlasie, Poland (53º 13ʹ 14.865" N 23º 51ʹ 9.327" E). The leaves, bark, and fruits were lyophilized (CHRIST 1-4 LSC freeze dryer -Martin Christ Gefriertrocknungsanlagen GmbH, Osterode am Harz, Germany).…”

“…We can also find organic acids such as citric acid, shikimic acid, malic acid, or quinic acid in its composition. Data on antioxidant properties of individual plant parts are available in the literature, [3][4][5][6] but they are not complete.…”