Polyphenol content and in vitro evaluation of antioxidant, antimicrobial and prebiotic properties of red fruit extracts

Coman, Maria Magdalena; Oancea, Ana Maria S.; Verdenelli, Maria Cristina; Cecchini, Cinzia; Bahrim, Gabriela; Orpianesi, Carla; Cresci, Alberto; Silvi, Stefania

doi:10.1007/s00217-017-2997-9

Cited by 65 publications

(37 citation statements)

References 29 publications

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“…An acidic environment (pH 4.5–6.5) is beneficial to the growth of Bifidobacteria and Lactobacillus [ 34 ]. Cy3glc and Cy3glc-C12 metabolites not only provided appropriate pH for the growth of intestinal probiotics, but also inhibited the growth of spoilage organisms [ 5 ].…”

Section: Resultsmentioning

confidence: 99%

“…After incubation for 24 h, essential nutrients were deficient in the medium, which might be responsible for their faster decay. Secondly, phenolic hydroxyl groups of Cy3glc and Cy3glc-C12 could react with mycoproteins or enzymes through hydrogen bonding, resulting in cytoplasm pyknosis and disintegration [5]. However, no significant difference of relative bacteria count was observed in Cy3glc and Cy3glc-C12 (p > 0.05) after 36 h of incubation.…”

mentioning

confidence: 94%

“…Up to now, there are more than 500 kinds of anthocyanins and 23 aglycons [2]. During the past decades, in vitro and in vivo studies have revealed that anthocyanins have health-promoting effects, including antioxidant, antineoplastic, anticarcinogenic, anti-inflammatory, antibacterial, and hepato-protective activities [3][4][5][6]. In our previous study, anthocyanins from black rice (recovery: 0.86%, purity: 95.93%) were extracted by buffer solutions, and were purified using membrane separation and resin adsorption [7].…”

Section: Introductionmentioning

confidence: 99%

“…An acidic environment (pH 4.5-6.5) is beneficial to the growth of Bifidobacteria and Lactobacillus [34]. Cy3glc and Cy3glc-C12 metabolites not only provided appropriate pH for the growth of intestinal probiotics, but also inhibited the growth of spoilage organisms [5]. Bifidobacteria decreased faster at their decline phase in Cy3glc and Cy3glc-C12 media, which could be attributed to two aspects: Firstly, the nutrients were quickly used up by Bifidobacteria due to the bioactivity of Cy3glc and Cy3glc-C12.…”

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confidence: 99%

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Kinetics of Enzymatic Synthesis of Cyanidin-3-Glucoside Lauryl Ester and Its Physicochemical Property and Proliferative Effect on Intestinal Probiotics

Yang

Sun

et al. 2020

Biology

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The interest in anthocyanins used in food, cosmetic, and pharmaceutical industries has increased the research in order to improve their stability while maintaining bioactivity. In this work, cyanidin-3-glucoside lauryl ester (Cy3glc-C12) was enzymatically synthesized, using Novozym 435 as a catalyst, as well as to obtain a kinetic model for the bioprocess. Its liposolubility, UV–VIS absorbance property, thermostability, and potential proliferative effect on intestinal probiotics were also studied. The maximum conversion yield (68.7 ± 2.1%) was obtained with a molar ratio (substrate:donor) of 1:56, 435 16.5 g/L Novozym, temperature of 56 °C, and a time of 28 h via the acylation occurred at 6²-OH position of the glucoside. The kinetics of the reaction is consistent with a ping-pong bi-bi mechanism and the parameters of the respective kinetic equations are reported. Compared with native Cy3glc, the liposolubility, pH resistivity and thermostability of Cy3glc-C12 were significantly improved. The growth kinetics of Bifidobacteria and Lactobacillus was established based on the Logistic equation, and Cy3glc-C12 could promote their proliferation especially during the logarithmic growth, in which lower pH and more bacteria population were found compared with those of media without anthocyanins. This research provided a reference for the industrial production of Cy3glc-C12 and extended its application to natural products in lipophilic systems.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Kinetics of Enzymatic Synthesis of Cyanidin-3-Glucoside Lauryl Ester and Its Physicochemical Property and Proliferative Effect on Intestinal Probiotics

Yang

Sun

et al. 2020

Biology

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“…2 Particularly, fruits and seeds have been studied as a good source of bio-active phytochemicals, especially for their antioxidant, anti-inflammatory, antimicrobial, and antitumor potentials. 3 , 4 Sour-cherry or Prunus cerasus ( P. cerasus ), from the family Rosaceae, is among the edible medicinal-plants which is used as a prophylactic agent against cardiovascular damage, Alzheimer's disorder, inflammatory diseases, and ameliorating a number of chronic ailments were marked by elevation in oxidative-stress like cancer, diabetes and neuropathy. 5 The fruit of P. cerasus is characterized by its content of polyphenols (like, anthocyanins and isoflavonoids), while its seed marked by its high content of fatty-acids (like, poly-saturated and poly-unsaturated fatty acids).…”

Section: Introductionmentioning

confidence: 99%

Gastroprotective and anti-inflammatory effects of Prunus cerasus phytochemicals and their possible mechanisms of action

Raafat

Darra

Saleh

2020

Journal of Traditional and Complementary Medicine

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Prunus cerasus ( P. cerasus ) is an alternative-medicine used traditionally for amelioration of chronic-ailments marked by elevation in oxidative-stress like neuropathy. The oxidative-stress control was reported to ameliorate the inflammatory-process. This study aimed to phytochemically-investigate P. cerasus most-active phytochemicals utilizing in-vivo biological models to explore their gastroprotective, anti-inflammatory, and antinociceptive potentials and their possible mechanisms of action. Sonication with EtAc was used to extract P. cerasus fruit (Scf), and seed (Scs). The phytochemical-investigation of Scf was performed by RP-HPLC, while that of Scs was explored utilizing GC-FID. A bio-guided-fraction and isolation method was done utilizing column-chromatography, and have shown that cyanidin-3-glucoside (Cy3G) was the most-active constituent in Scf, while linoleic-acid (LA) was the most-active constituent in Scs. Scf, Scs, Cy3G, and LA significantly (p ˂ 0.05) protected the gastric-mucosa against HCl/EtOH-induced gastric-lesions. Scs (200 mg/kg) has shown the most gastroprotective-potentials, and had comparable-results to ranitidine (50 mg/kg). Scf, Scs, Cy3G, and LA have shown significant anti-inflammatory and antinociceptive potentials against carrageenan induced-edema and nociceptive-pain, respectively, where Scs (200 mg/kg) has shown the most anti-inflammatory and antinociceptive potentials, and had comparable results to ibuprofen (100 mg/kg). Scf, Scs, Cy3G, and LA have counter-acted carrageenan-induced oxidative-stress markers, with increased serum-catalase and reduced-glutathione levels, and decreased lipid-peroxidation. Histopathological-studies demonstrated gastroprotective potentials, regeneration and improvement of the spleen-structural architecture when treated with highest doses of Scs and Scf. The reduction of the pro-inflammatory TNF-alpha and IL-6, and elevation the anti-inflammatory factor IL-10 levels, spleen regenerative-capacity and oxidative-stress amelioration might be the main-mechanism responsible for P. cerasus anti-inflammatory potentials. P. cerasus appears to aid in ameliorating the inflammatory process, and reducing pain-thresholds while preserving the stomach.

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Inactivation of Listeria monocytogenes and Salmonella Typhimurium in strawberry juice enriched with strawberry polyphenols

et al. 2020

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BACKGROUND: Low molecular-weight phenolic fractions (LMPFs) were extracted from Albion (LMPF-A) and Camarosa (LMPF-C) strawberry cultivars. Their antibacterial activity against Listeria monocytogenes and Salmonella Typhimurium cocktails in vitro and in vivo was investigated using strawberry juice as a food model. This study also sought to determine their antibacterial mechanism. RESULTS: Quercetin was identified as a principal compound in both phenolic fractions. The minimum bactericide concentration (MBC) values were 750 and 850 ∼g mL −1 (LMPF-C) and 800 and 950 ∼g mL −1 (LMPF-A) against S.Typhimurium and L. monocytogenes, respectively. The possible antibacterial activity of the phenolic extracts could be related to the release of phosphate and potassium ions, the effect of the disruption of membrane integrity on L. monocytogenes, and the effect of the inhibition of dihydronicotinamide adenine dinucleotide (NADH) oxidase activity on S. Typhimurium. Quercetin and kaempferol were the most active compounds in producing bacterial damage. Strawberry juice supplemented with the phenolic fractions and incubated at 37, 20, and 4°C reduced bacterial viability; moreover, after treatment with the phenolic fraction at the lowest temperature, no viable cells were detected after 7 days' incubation. Salmonella was more sensitive to the supplements than Listeria in strawberry juice. CONCLUSIONS: This study could form the basis for the development of natural antibacterial agents that could be included in natural juice or used by the pharmaceutical industry.

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Polyphenol content and in vitro evaluation of antioxidant, antimicrobial and prebiotic properties of red fruit extracts

Cited by 65 publications

References 29 publications

Kinetics of Enzymatic Synthesis of Cyanidin-3-Glucoside Lauryl Ester and Its Physicochemical Property and Proliferative Effect on Intestinal Probiotics

Kinetics of Enzymatic Synthesis of Cyanidin-3-Glucoside Lauryl Ester and Its Physicochemical Property and Proliferative Effect on Intestinal Probiotics

Gastroprotective and anti-inflammatory effects of Prunus cerasus phytochemicals and their possible mechanisms of action

Inactivation of Listeria monocytogenes and Salmonella Typhimurium in strawberry juice enriched with strawberry polyphenols

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