Polyphenol production in hairy root cultures of Fragaria x ananassa

Motomori, Yoshinao; Shimomura, Koichiro; Mori, Kinya; Kunitake, Hisato; Nakashima, Toshiki; Tanaka, Masanobu; Miyazaki, Sadami; Ishimaru, Kanji

doi:10.1016/0031-9422(95)00497-u

Cited by 13 publications

(4 citation statements)

References 10 publications

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“…In contrast, in roots, catechins and proanthocyanidins have only been studied in a few Rosaceae genera, mainly with respect to their pharmaceutical relevance: Fragaria vesca (Vennat et al. 1987) and hairy root cultures of Fragaria × ananassa (Motomori et al. 1995); rhizomes of Fragaria chiloensis (Simirgiotis & Schmeda‐Hirschmann 2010); Prunus armeniaca roots (Prasad 2000); and Aruncus sylvester, Potentilla alba, Geum rivale and Waldsteinia geoides (Oszmianski et al.…”

Section: Introductionmentioning

confidence: 99%

Histochemical screening, metabolite profiling and expression analysis reveal Rosaceae roots as the site of flavan‐3‐ol biosynthesis

Hoffmann¹,

Friedlhuber

Steinhauser

et al. 2011

Plant Biology

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Histochemical screening of 30 Rosaceae genera representing all classic subfamilies demonstrated flavan-3-ols (catechins) as general secondary metabolites in roots of Rosaceae. Semi-quantitative LC-MS analyses confirmed the presence of catechin, epicatechin and various dimeric flavan-3-ols (also representing higher polymeric proanthocyanidins) as prominent polyphenols in root tips of Fragaria (strawberry), Malus (apple), Rosa (rose), Pyrus (pear) and Prunus (plum). Distinct patterns of flavan-3-ol distribution at the cellular level were found in strawberry (Fragaria × ananassa) and apple (Malus × domestica) root tips. The calyptras (root caps) showed the most prominent flavan-3-ol staining for these two genera. Border cells of Fragaria and Malus, as first demonstrated here for Rosaceae, were also found to contain flavan-3-ols. Transcript analyses with cDNA demonstrated root expression of known flavonoid genes expressed in the respective fruits and leaves. Primarily, this proves in situ biosynthesis of flavan-3-ols in these roots. Knowledge of the distinct cellular distribution patterns and their in situ biosynthesis in roots provides a basis for analysis of the functional roles of Rosaceae root flavan-3-ols.

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Section: Introductionmentioning

confidence: 99%

Histochemical screening, metabolite profiling and expression analysis reveal Rosaceae roots as the site of flavan‐3‐ol biosynthesis

Hoffmann¹,

Friedlhuber

Steinhauser

et al. 2011

Plant Biology

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“…The beneficial effect of light on cyanidin 3- O -rutinoside accumulation was also observed in Tartary buckwheat HRs [ 34 ]. Motomori et al [ 35 ] studied polyphenolics accumulation in the HRs of Fragaria x ananassa Duch. cv.…”

Section: Polyphenolic Antioxidants In Hrsmentioning

confidence: 99%

“…The roots contained higher amounts of flavonols when exposed to light. Quercetin content reached its maximum (nearly 1 mg/g DW) 5–10 days after the transfer of roots to the fresh medium and then dropped, whereas the highest content of rutin was found after 15 days of culture (59 mg/g DW) [ 35 ]. Catechin and rutin contents in the HRs of Tartary buckwheat differed depending on the R. rhizogenes strain that was used to obtain the culture.…”

Section: Polyphenolic Antioxidants In Hrsmentioning

confidence: 99%

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Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

Malarz

Michalska

Yudina

et al. 2022

Plants

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Due to their chemical properties and biological activity, antioxidants of plant origin have gained interest as valuable components of the human diet, potential food preservatives and additives, ingredients of cosmetics and factors implicated in tolerance mechanisms against environmental stress. Plant polyphenols are the most prominent and extensively studied, albeit not only group of, secondary plant (specialized) metabolites manifesting antioxidative activity. Because of their potential economic importance, the productive and renewable sources of the compounds are desirable. Over thirty years of research on hairy root cultures, as both producers of secondary plant metabolites and experimental systems to investigate plant biosynthetic pathways, brought about several spectacular achievements. The present review focuses on the Rhizobium rhizogenes-transformed roots that either may be efficient sources of plant-derived antioxidants or were used to elucidate some regulatory mechanisms responsible for the enhanced accumulation of antioxidants in plant tissues.

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Using Hairy Roots for Production of Valuable Plant Secondary Metabolites

Tian

2015

Advances in Biochemical Engineering/Biotechnology

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Plants synthesize a wide variety of natural products, which are traditionally termed secondary metabolites and, more recently, coined specialized metabolites. While these chemical compounds are employed by plants for interactions with their environment, humans have long since explored and exploited plant secondary metabolites for medicinal and practical uses. Due to the tissue-specific and low-abundance accumulation of these metabolites, alternative means of production in systems other than intact plants are sought after. To this end, hairy root culture presents an excellent platform for producing valuable secondary metabolites. This chapter will focus on several major groups of secondary metabolites that are manufactured by hairy roots established from different plant species. Additionally, the methods for preservations of hairy roots will also be reviewed.

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Polyphenol production in hairy root cultures of Fragaria x ananassa

Cited by 13 publications

References 10 publications

Histochemical screening, metabolite profiling and expression analysis reveal Rosaceae roots as the site of flavan‐3‐ol biosynthesis

Histochemical screening, metabolite profiling and expression analysis reveal Rosaceae roots as the site of flavan‐3‐ol biosynthesis

Hairy Root Cultures as a Source of Polyphenolic Antioxidants: Flavonoids, Stilbenoids and Hydrolyzable Tannins

Using Hairy Roots for Production of Valuable Plant Secondary Metabolites

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