Polyphenols Content and Evaluation of Antioxidant Activity of Anacycluspyrethrum(l.) Lag. From Timahdite a Moroccan Middle Atlas Region.

Abstract

As part of valorisation of medicinal and aromatic plants from Timahdite, a region located in Moroccan Middle Atlas, we focused our study on an endemic species which belongstoAsteraceae family: Anacyclus pyrethrum L. This species, widely used by local people in traditional pharmacopeia, was chosen to conduct phytochemical studies and to assess the antioxidant activity of its roots, leaves and flowers extracts. Thus, the plant was harvested in May 2014. The selected organs (roots, leaves and flowers) of Anacyclus pyrethrum were sorted, cleaned and dried separately. Then, they were reduced into powder and stored away from light in a cool and dry place until use. Each organ was subjected to a phytochemical screening in order to qualitatively highlight its secondary metabolites. This qualitative analysis shows that roots are rich in alkaloids while the aerial parts (leaves and flowers) are rich in tannins and flavonoids. Extraction of total polyphenols was performed by maceration using methanol / water (70/30) as solvent. Yields were 2.30% for roots, 3.3% for leaves and 3.8% for flowers. Then, fractionation of hydro-methanol crude extract was carried out using successively three organic solvents of increasing polarity: chloroform, ethyl acetate and butanol. Quantitative analysis of total polyphenols conducted with Folin-Ciocalteu reagent showed that hydro-methanol crude extracts are richer in phenolic compounds than other fractions. Antioxidant activity of crude extracts determined through DPPH test was spectrophotometrically quantified and values of the half-maximal inhibitory concentrations (IC50) were graphically determined. These values are 77.62μggallic acid equivalent (GAE) / ml for flowers, 94.18μg / ml for leaves and 145.81μg / ml for roots, while 46.79μg / ml was found for ascorbic acid which was used as standard. Finally, a correlation was observed between total polyphenols content and antioxidant capacity determined by DPPH method: Aerial parts

Anacyclus pyrethrum var. pyrethrum (L.) and Anacyclus pyrethrum var. depressus (Ball) Maire: Correlation between Total Phenolic and Flavonoid Contents with Antioxidant and Antimicrobial Activities of Chemically Characterized Extracts

“…depressus (Ball) Maire. These findings were consistent with the results reported by Cherrat et al [ 49 ], which showed that extracts from genera Anacyclus contained polyphenol and flavonoid contents. The results of polyphenol and flavonoid contents found in the present study were lower than those reported by Sujith et al and Daoudi et al [ 15 , 50 ].…”

“…The Rm sample had the highest total antioxidant activity with a content of 708.74 ± 11.63 mg AAE/g, while the Cf sample had the lowest activity value (160.33 ± 10.23 AAE/g). These findings were in accordance with those reported in a work by Cherrat et al investigating chemical content in the genera Anacyclus [49].…”

“…Polyphenols have a very important role in determining the biological activities of natural products. Their contents are largely impacted by environmental conditions like harvest period, extraction solvent, and storage conditions [ 49 ]. Our findings showed that the hydro-alcoholic extracts of the two varieties (Fm and Ff) were higher in total phenolic and flavonoid compounds ( Table 4 ).…”

Phytochemistry, Biological and Pharmacological Activities of the Anacyclus pyrethrum (L.) Lag: A Systematic Review

“…The phytochemical examination of the leaves, flowers, roots and flower heads revealed the presence of alkaloids, reducing the compounds and catechins tannins. In addition, this species contains other chemicals such as gallic tannins, sterols, triterpenes, mucilage, coumarins, geese and holocides [ 27 , 32 ] as well as trace minerals such as Fe, Zn, Cr, Cu, Cd, Pb, and Ni [ 33 ]. The highest content of flavonoids and polyphenols is observed in the flowers compared to the leaves and roots.…”

Antiseizure effects of Anacyclus pyrethrum in socially isolated rats with and without a positive handling strategy

“…[14][15] Social isolation leads to a reduced seizure threshold in control animals [16][17] and produces a very severe phenotype in rodents with chronic epilepsy. 18 Because stressful situations-which may explain a more severe phenotype [19][20] -are associated consistently with oxidative stress [21][22] and inflammation, [23][24] and because extracts of A. pyrethrum have established antiinflammatory 25 and antioxidant properties, [25][26] we also tested whether the efficacy of the plant extracts was similar in experimental epilepsy in socially isolated rats with or without positive handling strategy. Since it is not practical to perform video-electroencephalography (EEG) recordings of individual rodents living in a colony, we used positive handling as an approximation of social contact.…”