Polyphenols from Blumea laciniata Extended the Lifespan and Enhanced Resistance to Stress in Caenorhabditis elegans via the Insulin Signaling Pathway

Abstract

Blumea laciniata is widely used as a folk medicine in Asia, but relevant literature on it is rarely reported. We confirmed that polyphenol extract (containing chlorogenic acid, rutin, and luteolin-4-O-glucoside) from B. laciniata (EBL) showed strong antioxidant ability in vitro. Hence, in this work, we applied Caenorhabditis elegans to further investigate the antioxidant and anti-ageing abilities of EBL in vivo. The results showed that EBL enhanced the survival of C. elegans under thermal stress by 12.62% and sharply reduced the reactive oxygen species level as well as the content of malonaldehyde. Moreover, EBL increased the activities of antioxidant enzymes such as catalase and superoxide dismutase. Additionally, EBL promoted DAF-16, a transcription factor, into the nucleus. Besides, EBL extended the lifespan of C. elegans by 17.39%, showing an anti-ageing effect. Different mutants indicated that the insulin/IGF-1 signaling pathway participated in the antioxidant and anti-ageing effect of EBL on C. elegans.

Polysaccharides from Brasenia schreberi with Great Antioxidant Ability and the Potential Application in Yogurt

“…Among these, the effect of 250 µg/mL was the best, and the surviv rate was increased to 82.3%, which was almost double than that in the control group, wh when the concentration of polysaccharides was over 500 µg/mL, especially 1000 µg/m the survival of worms was not significantly changed. A high concentration of polysacch rides was also shown to have some toxic effects on HUVEC cells, hence a higher conce The C. elegans is a good research model to evaluate the pharmacological activity of natural products like polysaccharides, polyphenols, flavone, and so on [30][31][32]. We found polysaccharides showed excellent protection of cells against H 2 O 2 -induced oxidative stress.…”

“…The C. elegans is a good research model to evaluate the pharmacological activity of natural products like polysaccharides, polyphenols, flavone, and so on [ 30 , 31 , 32 ]. We found polysaccharides showed excellent protection of cells against H 2 O 2 -induced oxidative stress.…”

“…Hence, we drew one conclusion that polysaccharides increased the cell viability against oxidative stress through reducing the ROS level and cell apoptosis, showing a good antioxidant ability in vitro. The C. elegans is a good research model to evaluate the pharmacological activity natural products like polysaccharides, polyphenols, flavone, and so on [30][31][32]. We fou polysaccharides showed excellent protection of cells against H2O2-induced oxidati stress.…”

Effect of Sodium Hyaluronate on Antioxidant and Anti-Ageing Activities in Caenorhabditis elegans

“…Based on a nematode model, purple pitanga fruit enhances resistance to H 2 O 2 oxidative stress and inhibits ROS [ 13 ]. Blumen laciniata polyphenols [ 14 ] and orange extracts [ 15 ] promote an anti-stress response and prolong lifespan. In addition, C. elegans have been entirely sequenced, and 60–80% of genes are high-homologous to humans.…”