Polyphosphate Accumulation among Denitrifying Bacteria in Activated Sludge

Jørgensen, Kirsten S.; Pauli, Anneli

doi:10.1006/anae.1995.1014

Cited by 51 publications

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“…Hascoet and Florentz (10), however, reported on a simultaneous uptake and release of phosphorus under anoxic conditions in the presence of an external carbon source, while van Niel et al (27) showed that, in the presence of nitrate, phosphorus release and acetate uptake by activated sludge organisms were severely inhibited. The studies described above, as well as most other studies on denitrification and phosphate removal in wastewater treatment plants (12,21), are based on the assumption that denitrifying PAOs with the physiological characteristics summarized in Table 1 are the only PAOs present in these environments. The present study demonstrates also that heterotrophic denitrifiers like P. denitrificans exhibit the ability to synthesize polyphosphate.…”

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“…Under these conditions, the PAOs release phosphorus in the anaerobic zone and store phosphorus as polyphosphate in the aerobic zone. Phosphorus is subsequently removed from the process stream by harvesting a fraction of the phosphorus-rich bacterial biomass (26).Recently, it was demonstrated that, not only under aerobic conditions but also under anoxic conditions, i.e., with nitrate as the electron acceptor, some PAOs are capable of polyphosphate accumulation (3,6,12,15). Since attempts to isolate bacteria with all characteristics attributed to PAOs have failed so far, information on these organisms is based on studies with crude sludge samples and enrichment cultures obtained from EBPR plants.…”

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Atypical Polyphosphate Accumulation by the Denitrifying Bacterium Paracoccus denitrificans

Barak

Rijn

2000

Appl Environ Microbiol

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Polyphosphate accumulation by Paracoccus denitrificans was examined under aerobic, anoxic, and anaerobic conditions. Polyphosphate synthesis by this denitrifier took place with either oxygen or nitrate as the electron acceptor and in the presence of an external carbon source. Cells were capable of poly-␤-hydroxybutyrate (PHB) synthesis, but no polyphosphate was produced when PHB-rich cells were incubated under anoxic conditions in the absence of an external carbon source. By comparison of these findings to those with polyphosphateaccumulating organisms thought to be responsible for phosphate removal in activated sludge systems, it is concluded that P. denitrificans is capable of combined phosphate and nitrate removal without the need for alternating anaerobic/aerobic or anaerobic/anoxic switches. Studies on additional denitrifying isolates from a denitrifying fluidized bed reactor suggested that polyphosphate accumulation is widespread among denitrifiers.Due to environmental problems associated with phosphorus discharge, phosphorus removal has become a standard treatment practice in wastewater purification plants. Enhanced biological phosphorus removal (EBPR) is the most common biological phosphorus removal method. The method is based on enrichment of so-called polyphosphate-accumulating organisms (PAOs) through recycling of the sludge between anaerobic and aerobic zones. Under these conditions, the PAOs release phosphorus in the anaerobic zone and store phosphorus as polyphosphate in the aerobic zone. Phosphorus is subsequently removed from the process stream by harvesting a fraction of the phosphorus-rich bacterial biomass (26).Recently, it was demonstrated that, not only under aerobic conditions but also under anoxic conditions, i.e., with nitrate as the electron acceptor, some PAOs are capable of polyphosphate accumulation (3,6,12,15). Since attempts to isolate bacteria with all characteristics attributed to PAOs have failed so far, information on these organisms is based on studies with crude sludge samples and enrichment cultures obtained from EBPR plants. On the basis of these studies, the following set of metabolic properties has been attributed to PAOs (22).Under anaerobic conditions, acetate or other low-molecularweight organic compounds are converted to polyhydroxyalkanoates (PHA), polyphosphate and glycogen are degraded, and phosphate is released. Under aerobic or anoxic conditions, PHA is converted to glycogen, phosphate is assimilated, and polyphosphate is intracellularly produced. Under the latter conditions, bacterial growth and phosphate uptake are regulated by the energy released from the breakdown of PHA.We studied the phosphorus dynamics in a prototype treatment system used for removal of organic matter and inorganic nitrogen from recirculating fish culture systems. The system comprised a nitrifying filter for oxidation of ammonia to nitrate, a digestion basin for anaerobic digestion of organic matter, and a denitrifying filter for reduction of nitrate to nitrogen gas (1). It was found that m...

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Atypical Polyphosphate Accumulation by the Denitrifying Bacterium Paracoccus denitrificans

Barak

Rijn

2000

Appl Environ Microbiol

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“…Seven groups of 26 morphological types of filamentous bacteria were isolated, cultivated and identified from activated sludge to achieve a better understanding of the complex phenomena of sludge bulking and foaming that influences the settleability and dewaterability of sludge [10] . A total of 165 denitrifying bacteria were isolated from activated sludge for polyphosphate accumulation and denitrification in biological removal of nutrient [11] . Several authors have been studied on biodiversity of bacterial community isolated from activated sludge [12][13][14] .…”

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confidence: 99%

Isolation and Purification of Bacterial Strains from Treatment Plants for Effective and Efficient Bioconversion of Domestic Wastewater Sludge

Jalal¹,

Alam²,

Muyibi³

et al. 2006

American J. of Environmental Sciences

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Forty six bacterial strains were isolated from nine different sources in four treatment plants namely Indah Water Konsortium (IWK) sewage treatment plant, International Islamic University Malaysia (IIUM) treatment plant-1,-2 and -3 to evaluate the bioconversion process in terms of efficient biodegradation and bioseparation. The bacterial strains isolated were found to be 52.2% (24 isolates) and 47.8% (22 isolates) in the IWK and IIUM treatment plants respectively. The results showed that the higher microbial population (9-10x10 4 cfu mL 1 ) was observed in the secondary clarifier of IWK treatment plant. Only the gram-staining identification was done in the strains isolated from IWK treatment plant not to be determined from IIUM. Among the isolates from IWK, 10 isolates of gram-positive bacillus (GPB) and gram-positive cocci (GPC), 10 isolates of gram-negative bacillus (GNB) and rest were both or undetermined. Gram-negative cocci (GNC) were not found in the isolates from IWK.

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“…Single carbon source Inoculating strain PK1 in the phosphorus uptake medium [6,7] and then cultivated in an OCXO (30°C, 120rpm/min). Changing the carbon source of phosphorus uptake medium and controlling the molar volume of carbon at the same level when different single carbon source (sodium acetate, sodium citrate and glucose) was used.…”

Section: Carbon Source Testsmentioning

confidence: 99%

Influence of nutrient factors on phosphorus accumulating organisms (PAOs) Bacillus sp. PK1

Zhang¹,

Yao²,

Li³

et al. 2018

Proceedings of the 2017 3rd International Forum on Energy, Environment Science and Materials (IFEESM 2017)

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A kind of phosphorus accumulating organism (PAO), Bacillus sp. PK1, which showed high phosphorus removal efficiencies, was isolated from special medium in the SBR reactor. To further explore the effect of nutrient factors on its potential phosphorus removal efficiency, initial phosphorus content and various carbon sources were selected in this research. Results of a series tests showed that the initial phosphorus removal efficiency declined constantly to below 60% with the initial phosphorus content increased. As for the effect of carbon sources, the phosphorus removal efficiencies were respectively as high as 98.58%, 95.14% and 98.45% with sodium acetate, sodium citrate and mixed carbon source. More importantly, research also showed an infrequent phenomenon that the PK1 gave priority to utilize sodium citrate rather than short chain fatty acid sodium acetate, and hardly to use glucose.

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Polyphosphate Accumulation among Denitrifying Bacteria in Activated Sludge

Cited by 51 publications

References 0 publications

Atypical Polyphosphate Accumulation by the Denitrifying Bacterium Paracoccus denitrificans

Atypical Polyphosphate Accumulation by the Denitrifying Bacterium Paracoccus denitrificans

Isolation and Purification of Bacterial Strains from Treatment Plants for Effective and Efficient Bioconversion of Domestic Wastewater Sludge

Influence of nutrient factors on phosphorus accumulating organisms (PAOs) Bacillus sp. PK1

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