Polyphosphate Granules and Acidocalcisomes

Jendrossek, Dieter

doi:10.1007/978-3-030-60173-7_1

Cited by 3 publications

(2 citation statements)

References 104 publications

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“…In the former, carbon is stored in the form of LDs, namely spherical organelles that are phase‐separated from the cytosol and densely packed with triacylglycerides and steryl esters [35, 36]. In the latter, carbon is stored in (prokaryotic) carbonosomes, polyhydroxyalkanoate granules such as poly(3‐hydroxybutyrate) (PHB) [37, 38].…”

Section: Resultsmentioning

confidence: 99%

“…In the former, carbon is stored in the form of LDs, namely spherical organelles that are phase-separated from the cytosol and densely packed with triacylglycerides and steryl esters [35,36]. In the latter, carbon is stored in (prokaryotic) carbonosomes, polyhydroxyalkanoate granules such as poly(3-hydroxybutyrate) (PHB) [37,38]. We found that scattering-based LSI can resolve both LDs in Y. lipolytica (Figure 5) and PHB granules in M. extorquens (Figure 6), matching the ROIs of these organelles as independently identified by fluorescence microscopy.…”

Section: Bioimagingmentioning

confidence: 99%

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Scattered‐light‐sheet microscopy with sub‐cellular resolving power

Subedi,

Stolyar,

Tuson

et al. 2023

Journal of Biophotonics

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Since its first demonstration over 100 years ago, scattering‐based light‐sheet microscopy has recently re‐emerged as a key modality in label‐free tissue imaging and cellular morphometry; however, scattering‐based light‐sheet imaging with subcellular resolution remains an unmet target. This is because related approaches inevitably superimpose speckle or granular intensity modulation on to the native subcellular features. Here, we addressed this challenge by deploying a time‐averaged pseudo‐thermalized light‐sheet illumination. While this approach increased the lateral dimensions of the illumination sheet, we achieved subcellular resolving power after image deconvolution. We validated this approach by imaging cytosolic carbon depots in yeast and bacteria with increased specificity, no staining, and ultralow irradiance levels. Overall, we expect this scattering‐based light‐sheet microscopy approach will advance single, live cell imaging by conferring low‐irradiance and label‐free operation towards eradicating phototoxicity.

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Section: Resultsmentioning

confidence: 99%

Section: Bioimagingmentioning

confidence: 99%

Scattered‐light‐sheet microscopy with sub‐cellular resolving power

Subedi,

Stolyar,

Tuson

et al. 2023

Journal of Biophotonics

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The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa

Chawla

Klupt

Patsalo

et al. 2022

mBio

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Migration of Polyphosphate Granules in Agrobacterium tumefaciens

et al. 2022

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Agrobacterium tumefaciens has two polyphosphate (polyP) kinases, one of which (PPK1AT) is responsible for the formation of polyP granules, while the other (PPK2AT) is used for replenishing the NTP pools by using polyP as a phosphate donor to phosphorylate nucleoside diphosphates. Fusions of eYFP with PPK2AT or of the polyP granule-associated phosin PptA from Ralstonia eutropha always co-localized with polyP granules in A. tumefaciens and allowed the tracking of polyP granules in time-lapse microscopy experiments without the necessity to label the cells with the toxic dye DAPI. Fusions of PPK1AT with mCherry formed fluorescent signals often attached to, but not completely co-localizing with, polyP granules in wild-type cells. Time-lapse microscopy revealed that polyP granules in about one-third of a cell population migrated from the old pole to the new cell pole shortly before or during cell division. Many cells de novo formed a second (nonmigrating) polyP granule at the opposite cell pole before cell division was completed, resulting in two daughter cells each having a polyP granule at the old pole after septum formation. Migration of polyP granules was disordered in mitomycin C-treated or in PopZ-depleted cells, suggesting that polyP granules can associate with DNA or with other molecules that are segregated during the cell cycle.

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Polyphosphate Granules and Acidocalcisomes

Cited by 3 publications

References 104 publications

Scattered‐light‐sheet microscopy with sub‐cellular resolving power

Scattered‐light‐sheet microscopy with sub‐cellular resolving power

The Histone H1-Like Protein AlgP Facilitates Even Spacing of Polyphosphate Granules in Pseudomonas aeruginosa

Migration of Polyphosphate Granules in <b><i>Agrobacterium tumefaciens</i></b>

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