Polyphosphate synthesis is an evolutionarily ancient phosphorus storage strategy in microalgae

Cliff, A; Guieysse, Benoı̂t; Brown, Nicola; Lockhart, Peter J.; Dubreucq, Éric; Plouviez, Maxence

doi:10.1016/j.algal.2023.103161

Cited by 13 publications

(24 citation statements)

References 57 publications

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“…Conversely, Plouviez et al [ 40 ] recently showed, by temporal analysis of both datasets, that the transcriptome and the proteome related to phosphorus metabolism are synchronized in Chlamydomonas . This provided insight into the turnover and regulation of Vacuolar Transport Chaperone proteins, key proteins involved in polyphosphate synthesis in microalgae [ 40 , 41 , 42 ].…”

Section: Proteomicsmentioning

confidence: 99%

“…In silico studies are also critical for fundamental research. In the case of microalgae, Cliff et al [ 41 ] used Alphafold 2 and molecular dynamics to generate the first models of VTC4 protein in C. reinhardtii , C. vulgaris , Scenedesmus sp. and Gonium pectorale , based on sequences from databases and their own sequencing data (example in Figure 3 ).…”

Section: Proteomics In the Post-omics Eramentioning

confidence: 99%

“…Prior to this study, the function of VTC4 as a polyP polymerase was only confirmed in C. reinhardtii , without information on its structure. However, as the models showed conservation of the VTC catalytic core and the SPX regulatory domains among the microalgae species and yeast, the study of Cliff et al [ 41 ] confirmed the probable function of VTC4 as a polyP polymerase in the other microalgae. In addition, by showing, through molecular docking calculations, the affinity between inositol phosphates and the SPX domain, this study also suggested a regulation of VTC4 proteins in microalgae that may be similar to that in yeast.…”

Section: Proteomics In the Post-omics Eramentioning

confidence: 99%

“…In addition, by showing, through molecular docking calculations, the affinity between inositol phosphates and the SPX domain, this study also suggested a regulation of VTC4 proteins in microalgae that may be similar to that in yeast. These findings have significant implications in microalgal biology and biotechnology, as polyPs have important metabolic functions in microalgae and significant research is focusing on the ability of microalgae to accumulate polyPs for P recovery from waste [ 4 , 40 , 41 , 42 , 139 ].…”

Section: Proteomics In the Post-omics Eramentioning

confidence: 99%

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Key Proteomics Tools for Fundamental and Applied Microalgal Research

Plouviez,

Dubreucq

2024

Proteomes

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Microscopic, photosynthetic prokaryotes and eukaryotes, collectively referred to as microalgae, are widely studied to improve our understanding of key metabolic pathways (e.g., photosynthesis) and for the development of biotechnological applications. Omics technologies, which are now common tools in biological research, have been shown to be critical in microalgal research. In the past decade, significant technological advancements have allowed omics technologies to become more affordable and efficient, with huge datasets being generated. In particular, where studies focused on a single or few proteins decades ago, it is now possible to study the whole proteome of a microalgae. The development of mass spectrometry-based methods has provided this leap forward with the high-throughput identification and quantification of proteins. This review specifically provides an overview of the use of proteomics in fundamental (e.g., photosynthesis) and applied (e.g., lipid production for biofuel) microalgal research, and presents future research directions in this field.

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Section: Proteomicsmentioning

confidence: 99%

Section: Proteomics In the Post-omics Eramentioning

confidence: 99%

Section: Proteomics In the Post-omics Eramentioning

confidence: 99%

Section: Proteomics In the Post-omics Eramentioning

confidence: 99%

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Key Proteomics Tools for Fundamental and Applied Microalgal Research

Plouviez,

Dubreucq

2024

Proteomes

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“…PolyP is an orthophosphate polymer widespread in nature (8)(9)(10)(11). The synthesis of polyP by C. reinhardtii is conjectured to be like Saccharomyces cerevsiae and occurs through the VTC complex in the vacuolar membrane (12,13). In S.cerevisiae, phosphate transported into the cell drives ATP synthesis, which in response triggers production of inositol phosphates (IPs).…”

Section: Introductionmentioning

confidence: 99%

Phosphate overplus response inChlamydomonas reinhardtii: polyphosphate dynamics to monitor phosphate uptake and turnover

Zúñiga-Burgos,

Saiardi,

Camargo-Valero

et al. 2024

Preprint

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Many micro-organisms store inorganic phosphate (Pi) in the form of polyphosphate (polyP) and exhibit in-cell polyP accumulation, a phenomenon known as ‘phosphate overplus response’, when resupplied with Pi after a period of deprivation. Quantitative and qualitative methods were used to follow the dynamics of polyP synthesis and turnover in four strains of Chlamydomonas reinhardtii during Pi deprivation followed by nutrient resupply. The lowest level of in-cell polyP during Pi deprivation, which also correlates with the cessation of growth, is the key parameter for the timing of Pi resupply to maximise the Pi overplus response Additional nutrients do not affect the size of the overplus response, but they are important for continued growth and maximal Pi removal from the media. Tracking polyP allows the correct time for nutrient resupply to be determined and therefore a reproducible Pi overplus response to be achieved. Depending on whether maximum cellular phosphorus (P) content or maximum Pi removal is desired different strategies may be required – e,g., Pi deprivation until growth cessation then resupplying complete nutrients gives the best trade-off between high in-cell P accumulation, high Pi uptake and algal biomass growth. Although polyP levels are maintained after Pi resupply, the polymer is dynamically remodelled. IP6increases during this time. This increase does not precede polyP synthesis as predicted by a model where inositol phosphates switch on polyP synthesis. One strain tested, CC-5325, shows enhanced Pi uptake and levels of polyP and total in-cell P, suggesting that strain selection is also important.

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