Polysaccharide isolated from the liquid culture broth of Inonotus obliquus suppresses invasion of B16-F10 melanoma cells via AKT/NF-κB signaling pathway

Abstract

A number of polysaccharides exhibit pharmacological activities. Polysaccharides derived from Inonotus obliquus (PLIO) appear to have various potential pharmacological properties, including anti‑tumor activity. However, the molecular mechanisms underlying these properties remain to be elucidated. The present study investigated the anti‑metastatic potential of PLIO and the underlying signaling pathways in B16‑F10 murine melanoma cells using the MTT colorimetric assay, in vitro migration and invasion assays, and flow cytometric and western blot analyses. PLIO inhibited the invasion of B16‑F10 cells and suppressed the expression of matrix metalloproteinases. PLIO treatment inhibited nuclear factor‑κB (NF‑κB) nuclear translocation in B16‑F10 cells. In addition, PLIO treatment inhibited the phosphorylation of c-Jun N‑terminal kinases and AKT. These results suggest that PLIO may suppress the invasion of highly metastatic melanoma cells via inhibition of the AKT/NF-κB signaling pathways.

Recent Developments in Inonotus obliquus (Chaga mushroom) Polysaccharides: Isolation, Structural Characteristics, Biological Activities and Application

“…Lee found that IOPS can effectively inhibit the migration and invasion of human non-small cell lung cancer A549 cells. Its possible mechanism might be that IOPS inhibits NF-κB nuclear translocation and JNK/AKT phosphorylation in A549 cells, and inhibits the AKT/NF-κB signaling pathway that reduces the expression level of matrix metalloproteinases (MMP); therefore, the invasion of human non-small cell lung cancer A549 cells was inhibited [ 96 , 97 ].…”

Bioactive compounds and bioactive properties of chaga ( Inonotus obliquus) mushroom: a review

“…The same authors proposed a similar mechanism (MAPKS, COX-2 and NF-κB pathway) on direct in vitro anti-migration, anti-invasion, and anti-proliferation activity on B16-F10 mouse melanoma cells (Lee et al, 2014b). However, the same authors later refuted their own proposed antimigration ability (Lee et al, 2016). While in vitro trial of LLC1 cell, the purified chaga polysaccharide showed direct cytotoxicity for activating AMPK via phosphorylation of threonine 172 by LKB1, downregulating Bcl-2 and upregulating Bax, as well as enhancing cleavage of Caspase-3 and PARP, leading to the opening of mitochondrial permeability transition pore, and reducing MMP, eventually resulting in an inhibition of ATP production and cellular proliferation (Jiang et al, 2019).…”

Inonotus obliquus – from folk medicine to clinical use

“… 109 Moreover, it has been reported that I. obliquus polysaccharides inhibit the expression of MMP-2, MMP-7, and MMP-9 in B16–F10 mouse cells. 93 Recently, Zhang et al. described that inotodiol from I. obliquus decreases the expression of MMP-2 and MMP-9 in HeLa human cells and reduces cell invasiveness.…”