Polysaccharides synthesised by micro-organisms

UDP-galactofuranose Precursor Required for Formation of the Lipopolysaccharide O Antigen of Klebsiella pneumoniae Serotype O1 Is Synthesized by the Product of the rfbDKPO1 Gene

“…However, development of strategies for inhibitors has been limited by the lack of fundamental information regarding the biosynthesis of galactofuranosyl residues. Galactocarolose, a ␤-D-(135)-linked polygalactofuranosid produced by P. charlesii, was the first polysaccharide found to contain galactofuranosyl residues (34,67). Trejo et al (46) isolated a nucleotide sugar formed in cell-free enzyme preparations of P. charlesii, which was capable of acting as a donor of galactofuranosyl residues in the biosynthesis of a galactofuranosylcontaining polymer.…”

Structure, mechanism, and dynamics of UDP-galactopyranose mutase

“…Nevertheless, it has been known for nearly a century that microorganisms produce Gal f in several forms. For example, galactocarolose, an extracellular β-D-(1→5)-linked polygalactofuranose produced by Penicillium charlesii , was the first polysaccharide shown to contain Gal f [1], motivating interest in the underlying biosynthetic pathway. Decades later, it was shown that P. charlesii could not use exogenous galactose to produce galactocarolose [2], leading to the discovery of a new nucleotide, UDP-Gal f , as the precursor in galactocarolose biosynthesis [3].…”

The specific substance from Pneumococcus type 34(41). The structure of a phosphorus-free repeating unit

“…The polysaccharide galactocarolose from Penicillium charle8ii (Haworth, Raistrick & Stacey, 1937;Gorin & Spencer, 1959), a polysaccharide from Gibberella fujikuroi (Siddiqui & Adams, 1961) and a galactomannan from Trychophyton granulosum (Bishop, Blank & Hranisavljevic-Jakovljevic, 1962) have all been shown to contain galactofuranose units. This is the first time such units have been reported as part of a pneumococcal antigen and nothing is yet known of their influence on the immunological specificity.…”