Polystyrene microparticles can affect the health status of freshwater fish – Threat of oral microplastics intake

Hollerová, Aneta; Hodkovicová, Nikola; Blahová, Jana; Faldyna, Martin; Franc, Aleš; Pavlkova, S.; Tichý, František; Poštulková, Eva; Mareš, Jan; Medkova, Denisa; Kyllar, M.; Svobodová, Zdeňka

doi:10.1016/j.scitotenv.2022.159976

Cited by 21 publications

(8 citation statements)

References 88 publications

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“…According to their characteristics, these particles can exist in the form of float, sink, and settle in water (De Villiers, 2018). There are micro and nanoplastics in all parts of aquatic ecosystems, but their effects on freshwater aquatic animals have not been investigated adequately, and most studies evaluated the effect of micro and nanoplastics on ecologically, economically, and conservationally important species (Hollerova et al, 2023; Schirinzi et al, 2020; Setala et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The present results proved that the expression of pro‐inflammatory cytokines, such as IL1β, IFN‐γ, and TNF‐α, increased by PS‐NPs that have negative impact on the immune system. Cytokines are secreted by immune cells to regulate the immune response and inflammation in the body during stressful conditions (Ahmadifar et al, 2021; Hollerova et al, 2023). Similar to our results, the IL1β expression rose in zebrafish (Lu et al, 2018), European sea bass larvae (Mazurais et al, 2015), and rainbow trout ( Oncorhynchus mykiss ) (Hollerova et al, 2023) after exposure to PS‐MPs.…”

Section: Discussionmentioning

confidence: 99%

“…Cytokines are secreted by immune cells to regulate the immune response and inflammation in the body during stressful conditions (Ahmadifar et al, 2021; Hollerova et al, 2023). Similar to our results, the IL1β expression rose in zebrafish (Lu et al, 2018), European sea bass larvae (Mazurais et al, 2015), and rainbow trout ( Oncorhynchus mykiss ) (Hollerova et al, 2023) after exposure to PS‐MPs. Our data demonstrated an increase in the TNF‐α gene expression along with increasing PS‐NP levels, which is in line with a report on rainbow trout (Hodkovicova et al, 2021).…”

Section: Discussionmentioning

confidence: 99%

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Assessing the impact of dietary polystyrene nanoplastics on growth performance, immunological parameters, and antioxidant defense in zebrafish (Danio rerio)

Ahmadifar,

Shohreh,

Kalhor

et al. 2024

J World Aquaculture Soc

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This trial was performed in order to investigate the response of zebrafish (Danio rerio) to dietary exposure to polystyrene nanoplastics (PS‐NPs) under laboratory conditions on fish growth and health. Healthy zebrafish (n=240) were divided into 12 tanks and fed with diets including 0 (T0), 100 (T1), 500 (T2), and 1000 (T3) mg kg−1 synthesized polystyrenes nanoplastics (PS‐NPs) for 30 days. At the end of trial, fish fed the PS‐NPs supplementation showed weight gain percentages of 79.45%, 70.35%, and 61.88% for T1, T2, and T3 groups, respectively, compared with 87.39% in the control fish. The SOD, GPX and MDA activities and cortisol levels increased by 47%, 32% and 35% and 46%, respectively, especially at high‐dose administration (p < 0.05). The expression of GPX (T2‐81% and T3‐82%) and SOD (T2‐101% and T3‐187%) were remarkably upregulated in T2 and T3 groups. Moreover, the relative gene expression of HSP70, interleukin‐1 (IL1), Interferon γ (IFN‐γ) and tumor necrosis factor‐α (TNF‐α) increased by 178%, 202%, 154% and 307%, respectively, especially at high‐dose administration (p < 0.05). The results of the present study demonstrated that exposure to PS‐NPs especially at high concentrations (500 and 1000 mg kg−1 of diet) negatively influenced growth, health status‐, antioxidant‐, and immunity‐related gene expression responses of zebrafish.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Assessing the impact of dietary polystyrene nanoplastics on growth performance, immunological parameters, and antioxidant defense in zebrafish (Danio rerio)

Ahmadifar,

Shohreh,

Kalhor

et al. 2024

J World Aquaculture Soc

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“…The procedure for coating and drying the samples was analogous to that in Chapter 2.1. Pellets were subjected to the examination of morphology employing scanning electron microscopy (SEM; MIRA3, Tescan Orsay Holding, Czech Republic) to visualize the surface and differences in its morphology ( 26 , 27 ).…”

Section: Methodsmentioning

confidence: 99%

Development and evaluation of a fish feed mixture containing the probiotic Lactiplantibacillus plantarum prepared using an innovative pellet coating method

et al. 2023

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IntroductionDue to the intensification of fish farming and the associated spread of antimicrobial resistance among animals and humans, it is necessary to discover new alternatives in the therapy and prophylaxis of diseases. Probiotics appear to be promising candidates because of their ability to stimulate immune responses and suppress the growth of pathogens.MethodsThe aim of this study was to prepare fish feed mixtures with various compositions and, based on their physical characteristics (sphericity, flow rate, density, hardness, friability, and loss on drying), choose the most suitable one for coating with the selected probiotic strain Lactobacillus plantarum R2 Biocenol™ CCM 8674 (new nom. Lactiplantibacillus plantarum). The probiotic strain was examined through sequence analysis for the presence of plantaricin- related genes. An invented coating technology based on a dry coating with colloidal silica followed by starch hydrogel containing L. plantarum was applied to pellets and tested for the viability of probiotics during an 11-month period at different temperatures (4°C and 22°C). The release kinetics of probiotics in artificial gastric juice and in water (pH = 2 and pH = 7) were also determined. Chemical and nutritional analyses were conducted for comparison of the quality of the control and coated pellets.Results and discussionThe results showed a gradual and sufficient release of probiotics for a 24-hour period, from 104 CFU at 10 mi up to 106 at the end of measurement in both environments. The number of living probiotic bacteria was stable during the whole storage period at 4°C (108), and no significant decrease in living probiotic bacteria was observed. Sanger sequencing revealed the presence of plantaricin A and plantaricin EF. Chemical analysis revealed an increase in multiple nutrients compared to the uncoated cores. These findings disclose that the invented coating method with a selected probiotic strain improved nutrient composition and did not worsen any of the physical characteristics of pellets. Applied probiotics are also gradually released into the environment and have a high survival rate when stored at 4°C for a long period of time. The outputs of this study confirm the potential of prepared and tested probiotic fish mixtures for future use in in vivo experiments and in fish farms for the prevention of infectious diseases.

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“…Similarly, PS is one of the most commonly produced plastics globally [ 19 ], with research revealing that PS microbeads can accumulate in sea urchin intestines and negatively affect their embryonic development [ 20 ]. Research has also found that ingestion of PS particles can cause damage to the liver and gills of rainbow trout, resulting in negative impacts on their health [ 21 ]. Scholars have also found that polystyrene microplastics can weaken the predator-induced defenses of Daphnia , which may have a certain impact on aquatic ecology [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

The Exploration of Joint Toxicity and Associated Mechanisms of Primary Microplastics and Methamphetamine in Zebrafish Larvae

Wang,

Xu,

Yuan

et al. 2024

Toxics

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The co-existence of microplastics (MPs) and methamphetamine (METH) in aquatic ecosystems has been widely reported; however, the joint toxicity and associated mechanisms remain unclear. Here, zebrafish larvae were exposed individually or jointly to polystyrene (PS) and polyvinyl chloride (PVC) MPs (20 mg/L) and METH (1 and 5 mg/L) for 10 days. The mortality, behavioral functions, and histopathology of fish from different groups were determined. PS MPs posed a stronger lethal risk to fish than PVC MPs, while the addition of METH at 5 mg/L significantly increased mortality. Obvious deposition of MPs was observed in the larvae’s intestinal tract in the exposure groups. Meanwhile, treatment with MPs induced intestinal deposits and intestinal hydrops in the fish, and this effect was enhanced with the addition of METH. Furthermore, MPs significantly suppressed the locomotor activation of zebrafish larvae, showing extended immobility duration and lower velocity. METH stimulated the outcome of PS but had no effect on the fish exposed to PVC. However, combined exposure to MPs and METH significantly increased the turn angle, which declined in individual MP exposure groups. RNA sequencing and gene quantitative analysis demonstrated that exposure to PS MPs and METH activated the MAPK signaling pathway and the C-type lectin signaling pathway of fish, while joint exposure to PVC MPs and METH stimulated steroid hormone synthesis pathways and the C-type lectin signaling pathway in zebrafish, contributing to cellular apoptosis and immune responses. This study contributes to the understanding of the joint toxicity of microplastics and pharmaceuticals to zebrafish, highlighting the significance of mitigating microplastic pollution to preserve the health of aquatic organisms and human beings.

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Polystyrene microparticles can affect the health status of freshwater fish – Threat of oral microplastics intake

Cited by 21 publications

References 88 publications

Assessing the impact of dietary polystyrene nanoplastics on growth performance, immunological parameters, and antioxidant defense in zebrafish (Danio rerio)

Assessing the impact of dietary polystyrene nanoplastics on growth performance, immunological parameters, and antioxidant defense in zebrafish (Danio rerio)

Development and evaluation of a fish feed mixture containing the probiotic Lactiplantibacillus plantarum prepared using an innovative pellet coating method

The Exploration of Joint Toxicity and Associated Mechanisms of Primary Microplastics and Methamphetamine in Zebrafish Larvae

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