Polystyrene microplastics induce endoplasmic reticulum stress, apoptosis and inflammation by disrupting the gut microbiota in carp intestines

Wang, Fuhan; Cui, Jie; Bao, Bowen; Deng, Xian; Liu, Lin; Guo, Mengyao

doi:10.1016/j.envpol.2023.121233

Cited by 56 publications

(16 citation statements)

References 34 publications

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“…An increase in GRP78 levels was observed in cells that were exposed to NPs, suggesting UPR activation (Figure D). This reflects the observations made in recent studies, where accumulation of NPs in the aquatic environment was found to disturb carp gut microbiota and induce UPR . Moreover, NPs have also been reported to increase expression of UPR-related genes such as GRP78, thereby inducing male reproductive toxicity .…”

Section: Resultssupporting

confidence: 89%

“…The GO and KEGG analyses showed the entire process of NPs’ cellular internalization and their behavior in the cell. After entering the cell by endocytosis, NPs accumulated in endosomes and then entered the lysosome for degradation or exited the cell directly as exosomes. , NPs could be released into the cytoplasm or other organelles by endosomal escape or lysosomal dysfunction, thereby affecting normal cellular function such as metabolism and protein processing in the endoplasmic reticulum (ER). , …”

Section: Resultsmentioning

confidence: 99%

“…38,43 NPs could be released into the cytoplasm or other organelles by endosomal escape or lysosomal dysfunction, 44 thereby affecting normal cellular function such as metabolism and protein processing in the endoplasmic reticulum (ER). 45,46 3.3. Metabolic Pathway Protein Adsorption and Functional Validation.…”

Section: Proteomic and Bioinformatics Analysis Of Specifically Adsorb...mentioning

confidence: 99%

“…This reflects the observations made in recent studies, where accumulation of NPs in the aquatic environment was found to disturb carp gut microbiota and induce UPR. 46 Moreover, NPs have also been reported to increase expression of UPR-related genes such as GRP78, thereby inducing male reproductive toxicity. 59 Therefore, we hypothesize that NPs may impair protein glycosylation in cells through RPN1 adsorption, which in turn impedes the proper folding of proteins and induces UPR.…”

Section: Genetic Information Processing Pathway Protein Adsorption An...mentioning

confidence: 99%

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Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells

Liu,

Wang,

Sheng

et al. 2024

Environ. Sci. Technol.

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Micro(nano)plastics (MNPs) are emerging pollutants that can adsorb pollutants in the environment and biological molecules and ultimately affect human health. However, the aspects of adsorption of intracellular proteins onto MNPs and its biological effects in cells have not been investigated to date. The present study revealed that 100 nm polystyrene nanoplastics (NPs) could be internalized by THP-1 cells and specifically adsorbed intracellular proteins. In total, 773 proteins adsorbed onto NPs with high reliability were identified using the proteomics approach and analyzed via bioinformatics to predict the route and distribution of NPs following cellular internalization. The representative proteins identified via the Kyoto Encyclopedia of Genes and Genomes pathway analysis were further investigated to characterize protein adsorption onto NPs and its biological effects. The analysis revealed that NPs affect glycolysis through pyruvate kinase M (PKM) adsorption, trigger the unfolded protein response through the adsorption of ribophorin 1 (RPN1) and heat shock 70 protein 8 (HSPA8), and are chiefly internalized into cells through clathrin-mediated endocytosis with concomitant clathrin heavy chain (CLTC) adsorption. Therefore, this work provides new insights and research strategies for the study of the biological effects caused by NPs.

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Section: Resultssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Proteomic and Bioinformatics Analysis Of Specifically Adsorb...mentioning

confidence: 99%

Section: Genetic Information Processing Pathway Protein Adsorption An...mentioning

confidence: 99%

See 2 more Smart Citations

Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells

Liu,

Wang,

Sheng

et al. 2024

Environ. Sci. Technol.

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“…Primary BA is synthesized in the liver and secreted into the intestine where bacteria transform them to produce secondary BA, whereas, conversely, BA can directly or indirectly regulate the composition of gut microbes through activation of innate immune genes in the small intestine. , MPs exposure has been shown to disrupt the structure of gut flora in fish, chickens, and mice. ,− In addition, the combined exposure of aged MPs and antibiotics to fish was reported to disrupt galactose and amino acid metabolism and primary BA biosynthesis pathway, which provided a comprehensive toxic behavior of MPs in the aquatic environment . These results suggested that MPs disrupt intestinal flora and affect BA metabolism in vivo .…”

Section: Introductionmentioning

confidence: 99%

Environmentally Relevant Concentrations of Microplastic Exposure Cause Cholestasis and Bile Acid Metabolism Dysregulation through a Gut-Liver Loop in Mice

Wen,

Sun,

Yang

et al. 2024

Environ. Sci. Technol.

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The massive production of plastics causes the ubiquitous existence of microplastics (MPs) in the biota, therefore, posing exposure risks and potential health concerns to human beings. However, the exact mechanisms of MPs-induced toxicities and abnormalities are largely unknown. In this study, we developed a mouse model of gavage polystyrene microplastics (PS MPs) for 30 days. We found that PS MPs can damage the intestinal barrier, accumulate in the liver tissue, and cause injury. The liver and intestine are both highly associated with bile acid (BA) metabolism. Indeed, we found that PS MPs dysregulate BA synthesis and efflux-related gene expression in the liver, causing cholestasis. Tandemly, PS MPs alter the ratio of primary to secondary BA in the feces by affecting the composition of the intestinal flora. At last, PS MPs alter mice’s fecal BA profile, which affects normal BA metabolism. Taken together, the present study provides robust data on the mechanism of toxicity of MPs causing the disturbance of BA metabolism via a 4-step gut-liver loop.

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Chondroitin Sulfate from Halaelurus burgeri Skin Inhibits Hepatic Endoplasmic Reticulum Stress and Inflammation, and Regulates Gut Microbiota

Ren,

Gao,

et al. 2024

Molecular Nutrition Food Res

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ScopePrevious study has demonstrated the chemical structure of chondroitin sulfate (CHS) from Halaelurus burgeri skin and its effects on insulin resistance. However, the precise impact of this phenomenon on endoplasmic reticulum (ER) stress and inflammation, which contribute to insulin resistance, remains unclear. This study is to investigate the impact of CHS on ER stress, inflammatory response and signaling, and gut microbiota in high‐fat diet (HFD)‐fed mice.Methods and resultsHFD‐fed C57BL/6J mice receive dietary gavage intervention of CHS for 18 weeks. Blood, liver tissue, and feces are harvested for further investigation. Results show that CHS inhibits ER stress, accompanied by lowered blood glucose, nitric oxide (NO), reactive oxygen (ROS), and free fatty acids (FFA) levels, and increases hepatic glycogen accumulation. Moreover, hepatic inflammation is improved by CHS treatment via inactivation of Toll‐like receptor 4 (TLR4) signaling and its downstream c‐jun N‐terminal kinase (JNK) and nuclear factor kappa B (NFκB) pathways. Additionally, CHS regulates gut microbiota, particularly the decline in the Firmicutes to Bacteroidetes ratio. CHS also lowers fecal lipopolysaccharide and elevates several fecal short chain fatty acids.ConclusionThese findings suggest that CHS from H. burgeri skin may be an alternative functional food supplement for anti‐ER stress, anti‐inflammtion, and regulation of gut microbiota.

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Polystyrene microplastics induce endoplasmic reticulum stress, apoptosis and inflammation by disrupting the gut microbiota in carp intestines

Cited by 56 publications

References 34 publications

Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells

Intracellular Protein Adsorption Behavior and Biological Effects of Polystyrene Nanoplastics in THP-1 Cells

Environmentally Relevant Concentrations of Microplastic Exposure Cause Cholestasis and Bile Acid Metabolism Dysregulation through a Gut-Liver Loop in Mice

Chondroitin Sulfate from Halaelurus burgeri Skin Inhibits Hepatic Endoplasmic Reticulum Stress and Inflammation, and Regulates Gut Microbiota

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