Polystyrene nanoplastics affect the human ubiquitin structure and ubiquitination in cells: a high-resolution study

Valle, Maria della; D’Abrosca, Gianluca; Gentile, Maria Teresa; Russo, Luigi; Isernia, Carla; Gaetano, Sonia Di; Avolio, Roberto; Castaldo, Rachele; Cocca, Mariacristina; Gentile, Gennaro; Malgieri, Gaetano; Errico, Maria Emanuela; Fattorusso, Roberto

doi:10.1039/d2sc04434j

Cited by 10 publications

(3 citation statements)

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“…Adapted with permission. [230] Copyright 2022, RSC. Recent research in dandelion (Taraxacum officinale) reported alteration in the 3D conformation of Rubisco, and DHA on interaction with virgin 80 nm PSNPs, and its carboxy and amino modifications.…”

Section: Pnps Induce the Tertiary Structural Aberrations In Proteinsmentioning

confidence: 99%

“…In understanding the secondary structural transition, the CD pattern indicated the modulation in protein secondary structure; however, it defied the typical model of α helix to β sheet/random coil transition (Figure 4b). [ 121 ] Nanoplastics have also been found to bind α amylase at multiple sites at chain A involving critical amino acids Trp163, Ala42, and Leu167, which leads to PNP concentration dependent secondary structural changes in the protein. [ 122 ] A recent study reported that PNPs enhance the relative abundance of α helix and 3‐turn helix as compared to the sum of antiparallel β‐sheet and random coil.…”

Section: Molecular Insights Into Protein‐pnp Interactionsmentioning

confidence: 99%

mentioning

confidence: 99%

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Mechanistic Insights into Cellular and Molecular Basis of Protein‐Nanoplastic Interactions

Naidu,

Nagar,

Poluri

2023

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Plastic waste is ubiquitously present across the world, and its nano/sub‐micron analogues (plastic nanoparticles, PNPs), raise severe environmental concerns affecting organisms’ health. Considering the direct and indirect toxic implications of PNPs, their biological impacts are actively being studied; lately, with special emphasis on cellular and molecular mechanistic intricacies. Combinatorial OMICS studies identified proteins as major regulators of PNP mediated cellular toxicity via activation of oxidative enzymes and generation of ROS. Alteration of protein function by PNPs results in DNA damage, organellar dysfunction, and autophagy, thus resulting in inflammation/cell death. The molecular mechanistic basis of these cellular toxic endeavors is fine‐tuned at the level of structural alterations in proteins of physiological relevance. Detailed biophysical studies on such protein‐PNP interactions evidenced prominent modifications in their structural architecture and conformational energy landscape. Another essential aspect of the protein‐PNP interactions includes bioenzymatic plastic degradation perspective, as the interactive units of plastics are essentially nano‐sized. Combining all these attributes of protein‐PNP interactions, the current review comprehensively documented the contemporary understanding of the concerned interactions in the light of cellular, molecular, kinetic/thermodynamic details. Additionally, the applicatory, economical facet of these interactions, PNP biogeochemical cycle and enzymatic advances pertaining to plastic degradation has also been discussed.

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Section: Pnps Induce the Tertiary Structural Aberrations In Proteinsmentioning

confidence: 99%

Section: Molecular Insights Into Protein‐pnp Interactionsmentioning

confidence: 99%

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Mechanistic Insights into Cellular and Molecular Basis of Protein‐Nanoplastic Interactions

Naidu,

Nagar,

Poluri

2023

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Genotoxic and neurotoxic potential of intracellular nanoplastics: A review

Casella,

Ballaz

2024

J of Applied Toxicology

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Plastic waste comprises polymers of different chemicals that disintegrate into nanoplastic particles (NPLs) of 1–100‐nm size, thereby littering the environment and posing a threat to wildlife and human health. Research on NPL contamination has up to now focused on the ecotoxicology effects of the pollution rather than the health risks. This review aimed to speculate about the possible properties of carcinogenic and neurotoxic NPL as pollutants. Given their low‐dimensional size and high surface size ratio, NPLs can easily penetrate biological membranes to cause functional and structural damage in cells. Once inside the cell, NPLs can interrupt the autophagy flux of cellular debris, alter proteostasis, provoke mitochondrial dysfunctions, and induce endoplasmic reticulum stress. Harmful metabolic and biological processes induced by NPLs include oxidative stress (OS), ROS generation, and pro‐inflammatory reactions. Depending on the cell cycle status, NPLs may direct DNA damage, tumorigenesis, and lately carcinogenesis in tissues with high self‐renewal capabilities like epithelia. In cells able to live the longest like neurons, NPLs could trigger neurodegeneration by promoting toxic proteinaceous aggregates, OS, and chronic inflammation. NPL genotoxicity and neurotoxicity are discussed based on the gathered evidence, when available, within the context of the intracellular uptake of these newcomer nanoparticles. In summary, this review explains how the risk evaluation of NPL pollution for human health may benefit from accurately monitoring NPL toxicokinetics and toxicodynamics at the intracellular resolution level.

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Nano-scale dangers: Unravelling the impact of nanoplastics on human trophoblast invasion

Nacka-Aleksić,

Vilotić,

Pirković

et al. 2025

Chemico-Biological Interactions

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Polystyrene nanoplastics affect the human ubiquitin structure and ubiquitination in cells: a high-resolution study

Abstract: A high-resolution study of ubiquitin–polystyrene nanoparticle (PS-NP) interaction is reported. We also show the effects of PS-NPs on ubiquitination in HeLa cells.

Cited by 10 publications

References 50 publications

Mechanistic Insights into Cellular and Molecular Basis of Protein‐Nanoplastic Interactions

Mechanistic Insights into Cellular and Molecular Basis of Protein‐Nanoplastic Interactions

Genotoxic and neurotoxic potential of intracellular nanoplastics: A review

Nano-scale dangers: Unravelling the impact of nanoplastics on human trophoblast invasion

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