Polytrichum commune L.ex Hedw ethyl acetate extract-triggered perturbations in intracellular Ca2+ homeostasis regulates mitochondrial-dependent apoptosis

Yuan, Wenjuan; Cheng, Xinqi; Wang, Pan; Jia, Yali; Liu, Quanhong; Tang, Wei; Wang, Xiaobing

doi:10.1016/j.jep.2015.07.002

Cited by 7 publications

(3 citation statements)

References 47 publications

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“…A number of plant-derived natural products have been investigated for their antitumor activities [ 21 , 23 , 56 ]. Recently, it has been reported that bryophytes can induce apoptosis and cell cycle arrests [ 19 , 57 ]. In this study, our results showed that MPEE inhibited HCC cell growth both in vitro and in vivo, which might induce cell cycle arrest and apoptosis of HCC cells through intrinsic- and ER stress-associated signaling pathways.…”

Section: Discussionmentioning

confidence: 99%

Marchantia polymorpha L. ethanol extract induces apoptosis in hepatocellular carcinoma cells via intrinsic- and endoplasmic reticulum stress-associated pathways

Zhou

Aipire

et al. 2021

Chin Med

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Background Marchantia polymorpha L. is a kind of Chinese herbal medicine and has various biological activities including antioxidant and antifungal. However, it is not clear about the antitumor effect and mechanism of M. polymorpha. We prepared M. polymorpha ethanol extract (MPEE) and investigated its antitumor effect on hepatocellular carcinoma cells both in vitro and in vivo. Methods The viability of hepatocellular carcinoma cells was detected by MTT assay. The distribution of cell cycle was analyzed by propidium iodide (PI) staining. The morphology of nuclei was observed by Hoechst 33258 staining. Apoptosis was detected by Annexin V/PI staining. JC-1 fluorescent probe and DCFH-DA were used to detect the mitochondrial membrane potential (ΔψM) and the level of reactive oxygen species (ROS), respectively. Caspase inhibitors were used to test the function of caspase in the induction of apoptosis. Quantitative real time polymerase chain reaction (qRT-PCR) and Western blot were used to evaluate the levels of mRNA and protein, respectively. Differentially expressed genes and signaling pathways were identified by transcriptome analysis. The H22 tumor mouse model was used to detect the antitumor effect of the extract. Results MPEE significantly suppressed the migration and growth of BEL-7404, HepG2 and H22 cells in a dose- and time-dependent manner through induction of apoptosis characterized by chromosomal condensation and cell cycle arrest at G0/G1 and G2/M phases. MPEE induced mitochondria-dependent apoptosis via upregulation of Bax and downregulation of Bcl-2 to reduce mitochondrial membrane potential and increase the release of cytochrome c. The levels of cleaved caspase-8 and -9 were significantly increased, which sequentially activated caspase-3 to cleave PARP. We further found that MPEE significantly increased ROS production and activated endoplasmic reticulum (ER) stress associated-apoptotic signaling pathway. Moreover, MPEE significantly inhibited H22 tumor growth in mouse model and improved the survival of tumor mice. Conclusion These results suggested that MPEE suppressed hepatocellular carcinoma cell growth through induction of apoptosis via intrinsic- and ER stress-associated pathways.

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Section: Discussionmentioning

confidence: 99%

Marchantia polymorpha L. ethanol extract induces apoptosis in hepatocellular carcinoma cells via intrinsic- and endoplasmic reticulum stress-associated pathways

Zhou

Aipire

et al. 2021

Chin Med

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“…Subsequently, Spjut et al 1986 [15] summarised the results of the first comprehensive study by NCI on the antitumour properties of extracts from 123 mosses, 13 liverworts, and 1 hornwort species, and reported that 75 species showed varying degrees of cytotoxicity against P388 lymphocytic leukaemia cells. From that time onwards, numerous studies have found that mosses [16][17][18][19][20][21][22][23][24][25][26] and crude liverwort extracts [25,[27][28][29] are cytotoxic for several cancer cell lines (Tables 1 and 2). The molecules responsible for the antiproliferative activity have been identified as terpenoids, bibenzyls, and macrocyclic (bis)bibenzyls, which are reported by various studies as cytotoxic for a wide range of cancer cells (Tables 1 and 2).…”

Section: Bryophytes and Their Antiproliferative Activitymentioning

confidence: 99%

Anti-Tumour Activities from Secondary Metabolites and Their Derivatives in Bryophytes: A Brief Review

Cianciullo

Cimmino

Maresca

et al. 2022

Applied Biosciences

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Bryophytes are a poorly studied group of land plants that have been used in traditional medicine as a multipurpose remedy for centuries. Due to their peculiar morphology and physiology, bryophytes synthesise a multitude of secondary metabolites with a wide range of nutraceutical and pharmaceutical activities. Research has highlighted that secondary metabolites in bryophytes can also act as antitumour agents. Several studies have shown that bryophyte extracts and pure metabolites are cytotoxic against many cancer cell lines. Interestingly, some of these molecules and their derivatives are capable of acting on a specific target in cancer cells. Some macrocyclic(bis)bibenzyls from bryophytes can inhibit P-glycoprotein, reverting multidrug resistant cancer cell phenotypes, induce depolymerization of tubulin, stimulate apoptotic pathways, and inhibit angiogenesis. This brief review aims to collect recent knowledge on secondary metabolites of bryophytes and their derivatives, which have demonstrated an interaction with different molecular processes in cancer cells.

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“…Polytrichum commune Hedw., commonly known as a hair-cap moss, has been used as a traditional Chinese medicine as it has anticancer activity (Fu et al 2009;Yuan et al 2015). It shows clear differentiation of water-conducting tissue (hadrom and leptom), which is analogous to vascular tissue (xylem and phloem) of higher plants (Eschrich and Steiner 1968).…”

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confidence: 99%

The complete plastome ofPolytrichum communeHedw. (Polytrichaceae, Bryophyta)

Jin

Zhu

2021

Mitochondrial DNA Part B

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Polytrichum commune, one of hair-cap mosses, is the type species of the genus Polytrichum Hedw. (Polytrichaceae). Here we present its complete plastome. The plastome of P. commune is successfully assembled from raw reads sequenced by HiSeq X ten system. Its total length is 126,323 bp consisting of four regions: large single copy (LSC) region (88,070 bp), small single copy (SSC) region (16,717 bp), and inverted repeats (IRs; 9,680 bp per each). It contains 128 genes (84 coding genes, eight rRNAs, and 36 tRNAs); nine genes (four rRNAs and five tRNAs) are duplicated in IR regions. The overall GC content is 28.9% and in the LSC, SSC and IR regions is 26.1%, 25.1%, and 45.5%, respectively. This plastome is an important sequence resource for further studies on the class Polytrichopsida.

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Polytrichum commune L.ex Hedw ethyl acetate extract-triggered perturbations in intracellular Ca2+ homeostasis regulates mitochondrial-dependent apoptosis

Cited by 7 publications

References 47 publications

Marchantia polymorpha L. ethanol extract induces apoptosis in hepatocellular carcinoma cells via intrinsic- and endoplasmic reticulum stress-associated pathways

Marchantia polymorpha L. ethanol extract induces apoptosis in hepatocellular carcinoma cells via intrinsic- and endoplasmic reticulum stress-associated pathways

Anti-Tumour Activities from Secondary Metabolites and Their Derivatives in Bryophytes: A Brief Review

The complete plastome ofPolytrichum communeHedw. (Polytrichaceae, Bryophyta)

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