Polyvalent guide RNAs for CRISPR antivirals

Bagchi, Rammyani; Tinker-Kulberg, Rachel; Salehin, Mohammad; Supakar, Tinku; Chamberlain, Sydney; Ligaba-Osena, Ayalew; Josephs, Eric A.

doi:10.1016/j.isci.2022.105333

iScience

2022

DOI: 10.1016/j.isci.2022.105333

|View full text |Cite

Polyvalent guide RNAs for CRISPR antivirals

Rammyani Bagchi¹,

Rachel Tinker-Kulberg²,

Mohammad Salehin³

et al.

Help me understand this report

View preprint versions

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

Publication Types

Select...

Article2

Relationship

Self Cite0

Independent2

Authors

Journals

Cited by 2 publications

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants

Zhan,

Zhang,

et al. 2024

Plants

View full text Add to dashboard Cite

Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas have been recognized as powerful genome-editing tools in diverse eukaryotic species, including plants, and thus hold great promise for engineering virus resistance in plants. Nevertheless, further attention is required regarding various issues associated with applying new powerful technologies in the field. This mini-review focuses on the recent advances in using CRISPR/Cas9 and CRISPR/Cas13 systems to combat DNA and RNA viruses in plants. We explored the utility of CRISPR/Cas for targeting the viral genome and editing host susceptibility genes in plants. We also provide insights into the limitations and challenges of using CRISPR/Cas for plant virus interference and propose individual combinatorial solutions. In conclusion, CRISPR/Cas technology has the potential to offer innovative and highly efficient approaches for controlling viruses in important crops in the near future.

show abstract

CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants

Zhan,

Zhang,

et al. 2024

Plants

View full text Add to dashboard Cite

show abstract

A CRISPR-Cas13b System Degrades SARS-CoV and SARS-CoV-2 RNA In Vitro

Andersson,

Azatyan,

Ekenberg

et al. 2024

Viruses

View full text Add to dashboard Cite

In a time of climate change, population growth, and globalization, the risk of viral spread has significantly increased. The 21st century has already witnessed outbreaks of Severe Acute Respiratory Syndrome virus (SARS-CoV), Severe Acute Respiratory Syndrome virus 2 (SARS-CoV-2), Ebola virus and Influenza virus, among others. Viruses rapidly adapt and evade human immune systems, complicating the development of effective antiviral countermeasures. Consequently, the need for novel antivirals resilient to viral mutations is urgent. In this study, we developed a CRISPR-Cas13b system to target SARS-CoV-2. Interestingly, this system was also efficient against SARS-CoV, demonstrating broad-spectrum potential. Our findings highlight CRISPR-Cas13b as a promising tool for antiviral therapeutics, underscoring its potential in RNA-virus-associated pandemic responses.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Polyvalent guide RNAs for CRISPR antivirals

Cited by 2 publications

References 49 publications

CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants

CRISPR/Cas: An Emerging Toolbox for Engineering Virus Resistance in Plants

A CRISPR-Cas13b System Degrades SARS-CoV and SARS-CoV-2 RNA In Vitro

Contact Info

Product

Resources

About