Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment

Abstract

A halotolerant, Gram-stain-negative, rod-shaped and light-pink-pigmented bacterial strain, PB3 T , was isolated from a pond sediment near a hexachlorocyclohexane-producing factory, located at Chinhat, Lucknow, India. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain PB3T formed a distinct phyletic clade along with the members of the genusPontibacter. The 16S rRNA gene sequence similarity with other members of the genus Pontibacter ranged from 94.5 to 98.9 %. The cells were motile, aerobic, and catalase-and oxidase-positive. The major fatty acids were iso-C 15:0 , iso-C 15:0 3-OH, iso-C 17:0 3-OH, C 16:1 !5c, summed feature 3 (C 16:1 !6c/C 16:1 !7c) and summed feature 4 (iso-C 17:1 I/ anteiso-C 17:1 B). The polar lipid profile of strain PB3 T showed the presence of phosphatidylethanolamine, an unidentified aminophospholipid, unknown aminolipids and other unknown polar lipids. DNA-DNA hybridization based homology of strain PB3 T with respect to its most closely related species, Pontibacter chinhatensis LP51 T , was 44.7 %. The DNA G+C content was 53.5 mol%. On the basis of these data, it is proposed that the isolate belongs to the genus Pontibacter and represents a novel species, for which the name Pontibacter mucosus is proposed. The type strain is PB3 T (=DSM 100162

Pontibacter mangrovi sp. nov., isolated from mangrove sediment

“…Key taxonomic characteristics that distinguish strain HB172049 T from closely related type species of the genus Pontibacter Strains: 1, HB172049 T ; 2, Pontibacter mucosus DSM100162 T ; 3, Pontibacter chinhatensis CCM 8436 T ; 4, Pontibacter niistensis NII-0905 T , 5, Pontibacter actiniarum DSM 19842 T . Data for columns 1-2 were investigated by the authors under the same laboratory conditions, columns 3-5 were published previously [1,7,30]. +, Positive; −, negative; nr, not reported.…”

“…At the time of writing, the genus comprised 34 species with validly published names (www.bacterio.net/pontibacter.html). Those species were mainly isolated from soil environments, some of which were from hexachlorocyclohexane-contaminated environments [3–9], and some were from marine environments [1, 10–13]. In this paper, we present the results of the taxonomic study of a novel Pontibacter strain isolated from a mangrove sediment sample in Hainan, PR China.…”

“…Fatty acid compositions of strain HB172049 T and the related type strains in the genus Pontibacter Strains: 1, HB172049 T (this study); 2, Pontibacter mucosus DSM100162 T (this study); 3, Pontibacter chinhatensis CCM 8436 T ; 4, Pontibacter niistensis NII-0905 T ; 5, Pontibacter actiniarum DSM 19842 T . Data for columns 3-5 were published previously[1,7,30]. -, Not detected; tr, trace amounts (<1 %); nr, not reported.…”

Pontibacter liquoris sp. nov. and Pontibacter vulgaris sp. nov., two novel bacteria isolated from the pit mud of Chinese liquor

“…As of May 2023, the genus Pontibacter comprised 42 validly published species (www.bacterio.net/genus/Pontibacter) [2]. Members of the genus Pontibacter are characterized as Gram-negative, rod-shaped, catalase-positive (except for Pontibacter qinzhouensis [3]), MK-7 as the predominant menaquinone, with very high amounts of iso-C 15 : 0 (except for Pontibacter aydingkolensis , Pontibacter beigongshangensis , Pontibacter deserti , Pontibacter niistensis and Pontibacter ummariensis [4–8]) and summed feature 4 (iso-C 17 : 1 I and/or anteiso-C 17 : 1 B), and a DNA G+C content range of 43.9–58.6 mol% [1, 3–39]. During investigation of the microbial diversity of the pit mud of the Chinese liquor, two novel strains (NBU2971 T and NBU2972 T ) were isolated.…”