Pontibacter populi sp. nov., isolated from the soil of a Euphrates poplar (Populus euphratica) forest

Abstract

A Gram-negative-staining, rod-shaped, non-motile and pink bacterial strain was isolated from the soil of a Populus euphratica forest located in Xinjiang, China. The strain, designated strain HYL7-15 T , was subjected to a taxonomic analysis using a polyphasic approach. 16S rRNA gene sequence analyses indicated that the isolate belonged to the phylum Bacteroidetes and was related to the genus Pontibacter, with sequence similarities ranging from 93.1 to 95.0 % with other species of the genus Pontibacter. Strain HYL7-15 T contained MK-7 as the predominant menaquinone and its DNA G+C content was 44.9 mol%. The major cellular fatty acids of the novel strain were iso-C 15 : 0 (16.49 %), iso-C 17 : 0 3-OH (10.96 %) and summed feature 4 (comprising anteiso-C 17 : 1 B and/or iso-C 17 : 1 I, 18.46 %). The major polar lipids were phosphatidylethanolamine (PE) and phosphatidylglycerol (PG); diphosphatidylglycerol (DPG), three unknown aminophospholipids (APLs) and two unknown phospholipids (PLs) were also detected. On the basis of the evidence presented, it is concluded that strain HYL7-15 T

Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov., isolated from the desert

“…Most members of this genus have gliding motility and phosphatidylethanolamine as a major polar lipid (Nedashkovskaya et al, 2005;Subhash et al, 2013a;Dwivedi et al, 2013;Kang et al, 2013). At the time of writing, 13 species with validly published names had been described, with members of the species commonly isolated from habitats such as solar salterns, hexachlorocyclohexane (HCH) dump sites, rhizospheric soils, desert soils and sea water (Subhash et al, 2013b;Dwivedi et al, 2013;Xu et al, 2012;Raichand et al, 2011;Zhou et al, 2007;Nedashkovskaya et al, 2005;Kang et al, 2013). Five species were originally isolated in India, including: Pontibacter niistensis NII-0909 T (Dastager et al, 2010), Pontibacter lucknowensis DM9 T (Dwivedi et al, 2013), Pontibacter odishensis JC130 T (Subhash et al, 2013b), Pontibacter ramchanderi LP43 T (Singh et al, 2013b) and Pontibacter indicus LP100 T (Singh et al, 2013a).…”

Pontibacter saemangeumensis sp. nov., isolated from seawater

“…While the profile showed a similar pattern of major polar lipids to P. xinjiangensis KACC 14209 T (Fig. S2) and strains of other Pontibacter species, such as P. actiniarum and P. populi (Xu et al, 2012), the profile of unknown lipids was somewhat different. Based on morphological, physiological and biochemical properties, and chemotaxonomic and molecular data, strain GCM0142…”

Pontibacter mucosus sp. nov., isolated from hexachlorocyclohexane-contaminated pond sediment

“…The species belonging to the genus Pontibacter are Gram-stain-negative, heterotrophic, aerobic, rod-shaped, motile by means of gliding or nonmotile, have MK-7 (menaquinone 7) as the predominant respiratory quinone and phosphatidylethanolamine as the major polar lipid. At the time of writing, 24 species of the genus Pontibacter have been isolated and classified from diverse niches such as desert soils, muddy water, seawater, hexachlorocyclohexane (HCH)-contaminated soil and pond sediments, forest soils, solar salterns, mountain soils, rhizosphere soils and also from marine actinians (Nedashkovskaya et al, 2005;Suresh et al, 2006;Zhou et al, 2007;Zhang et al, 2008;Dastager et al, 2010;Raichand et al, 2011;Xu et al, 2012;Dwivedi et al, 2013;Kang et al, 2013;Subhash et al, 2013Subhash et al, , 2014Singh et al, 2014Singh et al, , 2015Srinivasan et al, 2014;Mahato et al, 2015). However, out of the 24 known species of the genus Pontibacter, the names of three species, namely 'Pontibacter salisaro' (Joung et al, 2011), 'Pontibacter jeungdoensis' and 'Pontibacter rhizosphera' (Raichand et al, 2011), have yet to be validly published according to the List of Prokaryotic names with Standing in Nomenclature (http://www.…”