Poor Hypo-Osmotic Swelling Test Results from Cryopreserved Sperm Despite Preservation of Sperm Motility

Abstract

Cryopreservationithawing of sperm leads to decreased motile density. Most donor programs select for cryopreservation a male with a high initial motile density and if the post-thaw semen has a motile density over 10 x 106/ml, the specimen is thought to represent a fertile specimen. Some recent data suggest that males with normal motile densities but subnormal hypo-osmotic swelling (HOS) tests may be infertile. A study was thus performed to bee if males with an adequate motile density after cryopreservation may still demonstrate membrane damage as evidenced by decreased viability and a poor HOS test. The semen species from seven men with motile densities 2 10 X 106/ml after freezethawing were evaluated for HOS changes and viability. Despite preservation of normal motile density, all 7 men had HOS scores below 50% following cryopreservation (though all were significantly above this level pre-freeze). The mean viability and HOS scores prefreezing were 70 f 9.7 and 68.5 f 9.5, and post thaw they dropped to 33.7 + 6.9 and 32.8 k 6.2. These data suggest a mechanism for impaired fertility even with adequate motile density of a thawed specimen that had been cryopreserved.

Manganese Provides Antioxidant Protection for Sperm Cryopreservation that May Offer New Consideration for Clinical Fertility

“…However, computer assisted motility of bovine sperm, immediately after thawing is not correlated with fertility 23. Similarly Lapointe et al24 suggested the beneficial effect of 0.1 mM MnCl 2 for the maintainace of sperm motility without detrimental effects on mucus penetration and fertilizing ability and hypothesized that Mn ++ would have an effect on sperm cyclase activity that lead to increased Ca ++ concentration and motility.…”

Development of a rapid, sensitive, and reproducible laboratory test kit for the assessment of plasma membrane integrity of human sperm

“…These were the following: [1] double distilled water (ddH 2 O), [2] 0.05% NaCl, [3] 0.1% NaCl, [4] 0.2% NaCl, [5] 0.4% NaCl, and [6] solution A, as described by Jeyendran et al (2) or the WHO manual (3) (0.735 g sodium citrate dihydrate þ 1.351 g of fructose in 100 mL of ddH 2 O). Liquefied semen (50 mL) was mixed with 500 mL of different hypoosmotic solutions for 5 minutes at room temperature.…”

“…Though there have been divergent degrees of correlation reported between the HOS test and the different sperm evaluation parameters, the test has been recognized as the predictor of human sperm fertility (4)(5)(6)(7). Though it is a very simple and sensitive test, it has never been routinely practiced.…”

Espermatozoides caprinos criopreservados em meio à base de leite desnatado acrescido de glutationa reduzida

“…No entanto, com base nos resultados das sondas fluorescentes, esperava-se que esses gametas apresentassem sinais de hiperativação, devido à ocorrência de danos na membrana plasmática causados pela congelação (CHECK & CHECK, 1991), resultando no aumento da quantidade de espermatozoides capacitados (MAXWELL & WATSON, 1996).…”