Population genetic structure, migration, and polyploidy origin of a medicinal species <i>Gynostemma pentaphyllum</i> (Cucurbitaceae)

Zhang, Xiao; Su, Hui; Yang, Jia; Feng, Li; Li, Zhong-Hu; Zhao, Guifang

doi:10.1002/ece3.5618

Cited by 21 publications

(23 citation statements)

References 94 publications

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“…Currently, transcriptome derived SSR markers have been widely applied in population genetic analyses of medicinal plants, such as Picria felterrae ( Yan et al, 2019 ), Gynostemma pentaphyllum ( Zhang et al, 2019 ), and Perilla frutescens ( Sa et al, 2018 ). Researches on constructing core collections of medicinal plants using transcriptome derived SSRs are also quickly increased in recent years.…”

Section: Discussionmentioning

confidence: 99%

Constructing a Core Collection of the Medicinal Plant Angelica biserrata Using Genetic and Metabolic Data

Liu

Wang

et al. 2020

Front. Plant Sci.

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Angelica biserrata is an important medicinal plant in Chinese traditional medicine. Its roots, which are known as Duhuo in Chinese, are broadly applied to treat inflammation, arthritis, and headache. With increasing market demand, the wild resources of A. biserrata have been overexploited, and conservation, assessment of genetic resources and breeding for this species is needed. Here, we sequenced the transcriptome of A. biserrata and developed simple sequence repeat (SSR) markers from it to construct a core collection based on 208 samples collected from Changyang-related regions. A total of 132 alleles were obtained for 17 SSR loci used with the polymorphic information content (PIC) ranging from 0.44 to 0.83. Abundant genetic diversity was inferred by Shannon’s information index (1.51), observed (0.57) and expected heterozygosity (0.72). The clustering analysis resulted into two sample groups and analysis of molecular variance (AMOVA) showed only 6% genetic variation existed among populations. A further metabolic analysis of these samples revealed the main coumarin contents, such as osthole and columbianadin. According to the genetic and metabolic data, we adopted the least distance stepwise sampling strategy to construct seven preliminary core collections, of which the 20CC collection, which possessed 42 A. biserrata individuals accounting for 90.20% of the genetic diversity of the original germplasm, represented the best core collection. This study will contribute to the conservation and management of A. biserrata wild germplasm resources and provide a material basis for future selection and breeding of this medicinal plant.

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Section: Discussionmentioning

confidence: 99%

Constructing a Core Collection of the Medicinal Plant Angelica biserrata Using Genetic and Metabolic Data

Liu

Wang

et al. 2020

Front. Plant Sci.

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“…It can be found as a health supplement in beverages, biscuits, face washes, and bath oils (Li et al, 2016). The herb has a low level of genetic diversity and a high level of variation among populations (Zhang et al, 2019).…”

Section: Gynostemma Pentaphyllummentioning

confidence: 99%

Herbal Medicine for Cardiovascular Diseases: Efficacy, Mechanisms, and Safety

et al. 2020

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Cardiovascular diseases (CVDs) are a significant health burden with an ever-increasing prevalence. They remain the leading causes of morbidity and mortality worldwide. The use of medicinal herbs continues to be an alternative treatment approach for several diseases including CVDs. Currently, there is an unprecedented drive for the use of herbal preparations in modern medicinal systems. This drive is powered by several aspects, prime among which are their cost-effective therapeutic promise compared to standard modern therapies and the general belief that they are safe. Nonetheless, the claimed safety of herbal preparations yet remains to be properly tested. Consequently, public awareness should be raised regarding medicinal herbs safety, toxicity, potentially lifethreatening adverse effects, and possible herb-drug interactions. Over the years, laboratory data have shown that medicinal herbs may have therapeutic value in CVDs as they can interfere with several CVD risk factors. Accordingly, there have been many attempts to move studies on medicinal herbs from the bench to the bedside, in order to effectively employ herbs in CVD treatments. In this review, we introduce CVDs and their risk factors. Then we overview the use of herbs for disease treatment in general and CVDs in particular. Further, data on the ethnopharmacological therapeutic potentials and medicinal properties against CVDs of four widely used plants, namely Ginseng, Ginkgo biloba, Ganoderma lucidum, and Gynostemma pentaphyllum, are gathered and reviewed. In particular, the employment of these four plants in the context of CVDs, such as myocardial infarction, hypertension, peripheral vascular diseases, coronary heart disease, cardiomyopathies, and dyslipidemias has been reviewed, analyzed, and critically discussed. We also endeavor to document the recent studies aimed to dissect the cellular and molecular cardio-protective mechanisms of the four plants, using recently reported in vitro and in vivo studies. Finally, we reviewed and reported the results of the

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“…Hence, the high F ST (0.596) and the low gene flow (0.346) found in this study contributed to the observed genetic structure, supported by significant IBD patterns in the study area (r = 0.448, P = 0.004). Zhang et al (2019) submitted that asexual propagation would also reduce genetic differences among individuals within populations and increase differences among populations.…”

Section: Population Genetic Structurementioning

confidence: 99%

The level of genetic diversity and differentiation of tropical lotus, Nelumbo nucifera Gaertn. (Nelumbonaceae) from Australia, India, and Thailand

et al. 2020

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Background: Nelumbo nucifera Gaertn., a perennial aquatic macrophyte species, has been cultivated in several Asian countries for its economic importance, and medicinal uses. Two distinct ecotypes of the species are recognized based on the geographical location where the genotypes are adapted, i.e., tropical lotus and temperate lotus. The genetic diversity levels and differentiation of the tropical lotus from poorly studied geographic regions still remain unclear. Here, the population genetic diversity and structure of 15 tropical lotus populations sampled from the previous understudied natural distribution ranges, including India, Thailand, and Australia, were assessed using nine polymorphic SSR markers. Results: The SSR markers used to genotype the 216 individuals yielded 65 alleles. The highest and lowest genetic diversity estimates were found in Thailand and Indian populations, respectively. STRU CTU RE analysis revealed three distinct genetic clusters, with relatively low admixtures, supported by PCoA cluster analysis. Low levels of gene flow (mean N m = 0.346) among the three genetic clusters signified the Mantel test for isolation by distance, revealing the existence of a positive correlation between the genetic and geographic distances (r = 0.448, P = 0.004). Besides, AMOVA analysis revealed a higher variation among populations (59.98%) of the three groups. Overall, the populations used in this study exposed a high level of genetic differentiation (F ST = 0.596). Conclusions: The nine polymorphic microsatellite markers used in our study sufficiently differentiated the fifteen tropical N. nucifera populations based on geography. These populations presented different genetic variability, thereby confirming that populations found in each country are unique. The low genetic diversity (H E = 0.245) could be explained by limited gene flow and clonal propagation. Conserving the available diversity using various conservation approaches is essential to enable the continued utilization of this economically important crop species. We, therefore, propose that complementary conservation approaches ought to be introduced to conserve tropical lotus, depending on the genetic variations and threat levels in populations.

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Population genetic structure, migration, and polyploidy origin of a medicinal species Gynostemma pentaphyllum (Cucurbitaceae)

Cited by 21 publications

References 94 publications

Constructing a Core Collection of the Medicinal Plant Angelica biserrata Using Genetic and Metabolic Data

Constructing a Core Collection of the Medicinal Plant Angelica biserrata Using Genetic and Metabolic Data

Herbal Medicine for Cardiovascular Diseases: Efficacy, Mechanisms, and Safety

The level of genetic diversity and differentiation of tropical lotus, Nelumbo nucifera Gaertn. (Nelumbonaceae) from Australia, India, and Thailand

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