Population structure and genetic diversity of the only extant Baroninae swallowtail butterfly, Baronia brevicornis, revealed by ISSR markers

Machkour‐M’Rabet, Salima; Leberger, Roxanne; León‐Cortés, Jorge L.; Gers, Charles

doi:10.1007/s10841-014-9647-3

Cited by 10 publications

(9 citation statements)

References 45 publications

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“…The levels of polymorphism found through ISSRs in the species of the Enantia jethys complex were equal or higher than those reported by other authors for butterflies [ 28 , 30 – 31 , 61 ]. Heterogeneous environments and gene exchange among populations (high connectivity) may lead to high levels of genetic variability (i.e., polymorphism) [ 62 – 63 ], for example P = 100% [ 26 ].…”

Section: Discussioncontrasting

confidence: 64%

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Molecular evidence of hybridization in sympatric populations of the Enantia jethys complex (Lepidoptera: Pieridae)

et al. 2018

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Hybridization events are frequently demonstrated in natural butterfly populations. One interesting butterfly complex species is the Enantia jethys complex that has been studied for over a century; many debates exist regarding the species composition of this complex. Currently, three species that live sympatrically in the Gulf slope of Mexico (Enantia jethys, E. mazai, and E. albania) are recognized in this complex (based on morphological and molecular studies). Where these species live in sympatry, some cases of interspecific mating have been observed, suggesting hybridization events. Considering this, we employed a multilocus approach (analyses of mitochondrial and nuclear sequences: COI, RpS5, and Wg; and nuclear dominant markers: inter-simple sequence repeat (ISSRs) to study hybridization in sympatric populations from Veracruz, Mexico. Genetic diversity parameters were determined for all molecular markers, and species identification was assessed by different methods such as analyses of molecular variance (AMOVA), clustering, principal coordinate analysis (PCoA), gene flow, and PhiPT parameters. ISSR molecular markers were used for a more profound study of hybridization process. Although species of the Enantia jethys complex have a low dispersal capacity, we observed high genetic diversity, probably reflecting a high density of individuals locally. ISSR markers provided evidence of a contemporary hybridization process, detecting a high number of hybrids (from 17% to 53%) with significant differences in genetic diversity. Furthermore, a directional pattern of hybridization was observed from E. albania to other species. Phylogenetic study through DNA sequencing confirmed the existence of three clades corresponding to the three species previously recognized by morphological and molecular studies. This study underlines the importance of assessing hybridization in evolutionary studies, by tracing the lineage separation process that leads to the origin of new species. Our research demonstrates that hybridization processes have a high occurrence in natural populations.

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Section: Discussioncontrasting

confidence: 64%

“…The ISSRs are dominant molecular markers, where the absence of a band is interpreted as the loss of a locus/allele through either the deletion of the SSR site or a chromosomal rearrangement [ 24 ]. The use of ISSR to study species of Lepidoptera is relatively recent, yet abundant (not an exhaustive list: [ 25 – 31 ]).…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence of hybridization in sympatric populations of the Enantia jethys complex (Lepidoptera: Pieridae)

et al. 2018

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“…The Inter Simple Sequence Repeats (ISSR)-PCR method is employed on plants and animals. This technique is largely employed for genetic population and phylogenetic studies between closely-related species [16,[43][44][45][46]. It provides highly reproducible results and generates abundant polymorphism in many systems.…”

Section: Issr-pcr Amplificationmentioning

confidence: 99%

“…In addition, microsatellites are inherently difficult to use in butterflies, partially due to the high frequency of null alleles [49]. Therefore, ISSR genetic markers have been widely applied in studies on butterfly population genetic [15][16][17]43,48,[50][51][52][53][54][55][56][57].…”

Section: Issr-pcr Amplificationmentioning

confidence: 99%

“…However, recent studies have contradicted this assertion, demonstrating that natural processes of fragmentation can strongly affect population structure. Machkour-M'Rabet et al (2014) [16] presented evidence that a natural barrier, such as a major river, can result in more differences between nearby populations of Baronia brevicornis Salvin, 1893 (Lepidoptera: Papilionidae) than large geographic distances within a homogeneous landscape. Leidner and Haddad (2010) [17] provided evidence that natural barriers affect the dispersal of the coastal endemic crystal skipper butterfly (Atrytonopsis quinteri Burns, 2015; Lepidoptera: Hesperiidae) to a larger degree than urbanization.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Climatic Conditions and Land Cover on Genetic Structure and Diversity of Eunica tatila (Lepidoptera) in the Yucatan Peninsula, Mexico

et al. 2018

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Fragmentation is the third cause of the biodiversity declination. Population genetic studies using Lepidoptera as the model species in the context of loss of habitat are scarce, particularly for tropical areas. We chose a widespread butterfly from Mexico as the model species to explore how changes of habitat characteristics (undisturbed forest, anthropogenic disturbances, and coastal areas), and climatic conditions affect genetic diversity and population structure. The Nymphalidae Eunica tatila is a common species in the Yucatan Peninsula considered to be a bio-indicator of undisturbed tropical forest, with migratory potential and a possible sex-biased dispersal. We genotyped 323 individuals collected in eight undisturbed areas, using four Inter Simple Sequence Repeats primers. Results show a high genetic diversity and no population structure. Temperature and shrub density present a positive and significant relationship with polymorphism values. Furthermore, our results show the positive effect of surrounding forest habitat on genetic diversity, confirming that E. tatila is a bio-indicator of undisturbed tropical forest. We found evidence of sex-biased dispersal. This paper represents one of the few studies on population genetics of tropical butterfly in a fragmented landscape and is, therefore, an important step in understanding the impact of habitat fragmentation on the risk of a butterflies’ decline.

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Insect Diversity Changes in Mexican Agro-landscapes

León-Cortés,

del-Val,

Boege

2024

Insect Decline and Conservation in the Neotropics

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Population structure and genetic diversity of the only extant Baroninae swallowtail butterfly, Baronia brevicornis, revealed by ISSR markers

Cited by 10 publications

References 45 publications

Molecular evidence of hybridization in sympatric populations of the Enantia jethys complex (Lepidoptera: Pieridae)

Molecular evidence of hybridization in sympatric populations of the Enantia jethys complex (Lepidoptera: Pieridae)

Effect of Climatic Conditions and Land Cover on Genetic Structure and Diversity of Eunica tatila (Lepidoptera) in the Yucatan Peninsula, Mexico

Insect Diversity Changes in Mexican Agro-landscapes

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