Population Structure of <i>Pseudocercospora fijiensis</i> in Costa Rica Reveals Shared Haplotype Diversity with Southeast Asian Populations

Saville, Amanda; Charles, Melodi; Chavan, Suchitra; Muñoz, Melania; Gómez-Alpízar, Luis; Ristaino, Jean Beagle

doi:10.1094/phyto-02-17-0045-r

Cited by 4 publications

(2 citation statements)

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“…Another challenge is the threat of diseases to crop yield. For instance, Pseudocercospora fijiensis, the causal pathogen of black Sigatoka, a devastating disease of banana, can cause 20 to 80% yield loss in the absence of fungicides in banana crops [16].…”

Section: Current State Of the Southeast Asian Agricultural Industrymentioning

confidence: 99%

Utilizing Resources and Reducing Emissions through Waste-to-Energy Technologies in Food Processing in the Southeast Asian Agricultural Industry

Lasaksi

2023

WSIS

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The application of waste-to-energy (WTE) technology in Southeast Asian food processing is examined in this study, with an emphasis on emissions reduction and better resource usage. To evaluate the social, environmental, and economic effects, quantitative evaluations were carried out at a few chosen facilities. The findings show that there has been a 60% decrease in greenhouse gas emissions, a 25% gain in energy efficiency, and a 40% reduction in the production of organic waste on a daily basis. Positive returns on an initial investment of $5 million on average, together with the creation of 200 jobs per facility, are indicators of economic feasibility. The research highlights the revolutionary possibilities of WTE technology and offers practical suggestions for the area's sustainable growth.

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Section: Current State Of the Southeast Asian Agricultural Industrymentioning

confidence: 99%

Utilizing Resources and Reducing Emissions through Waste-to-Energy Technologies in Food Processing in the Southeast Asian Agricultural Industry

Lasaksi

2023

WSIS

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“…Anamorphs and teleomorphs are both present on infected leaves, and the ascospores produced during the sexual stage play an important epidemiological role through windborne dispersal (Marin et al, 2003). Understanding the genetic structure of fungal populations and how it evolves in the pathogen is an important aspect for the development of strategies in plant breeding and the management of disease resistance (Saville et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Genetic variability of Pseudocercospora fijiensis, the black Sigatoka pathogen of banana (Musa spp.) in Mexico

et al. 2019

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Pseudocercospora (previously known as Mycosphaerella) fijiensis causes black Sigatoka disease in banana (Musa spp.) and is considered to be the most devastating pathogen of this crop worldwide. To improve knowledge of its evolutionary patterns, this study determined the genetic variability of populations from two regions of Mexico: Central Pacific (Colima and Michoacan) and Southern (Chiapas, Tabasco and Oaxaca), using 10 simple sequence repeat (SSR) loci and the MAT‐specific PCR assay. Both mating types were present in all regions under study, with frequencies of 63% MAT1‐1 and 37% MAT1‐2. The SSR markers showed an average of three alleles per locus, resulting in 34 alleles in total. The genetic diversity (HT) was 0.3308, but at the local level (HS) ranged from 0.0976 (Colima) to 0.2228 (Oaxaca). However, the genotypic diversity was usually high (H′ > 2.4, S > 0.89). Cluster analysis grouped the isolates into five clusters with high statistical support (au > 80%), suggesting a geographic organization of the genetic variability of P. fijiensis; AMOVA, the minimum spanning tree and the population structure analysis supported this result, and all data indicated that the major genetic differences were between the different populations under analysis. Thus, the high level of genetic variability in P. fijiensis is attributed partly to a high rate of sexual reproduction, and also to a strong evolutionary capacity coupled with isolation due to limited genetic flow between distant populations. Both possibilities could be playing a relevant role in population differentiation of the pathogen.

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Genetic Diversity and Mating Type Distribution of Pseudocercospora fijiensis on Banana in Uganda and Tanzania

et al. 2021

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Black Sigatoka, caused by Pseudocercospora fijiensis, is a major foliar disease of banana and plantain worldwide. Little information on the genetic diversity and population structure of the pathogen is available for East Africa, which is needed to design effective and durable disease management strategies. We genotyped 319 single-spore isolates of P. fijiensis collected from seven regions in Uganda and Tanzania and five from Nigeria using 16 simple sequence repeat markers and mating type specific primers. Isolates from each country and region within country were treated as populations and subpopulations, respectively. A total of 296 multi-locus genotypes (MLGs) were recovered, representing a clonal fraction of 7%. Sub-populations had a moderate level of genetic diversity (Hexp = 0.12-0.31; mean 0.29). Mating type distribution did not deviate from equilibrium (MAT1-1: MAT1-2, 1:1 ratio) in Uganda, but in Tanzania the mating types were not in equilibrium (4:1 ratio). Index of association tests (IA and r̄d) showed that all populations were at linkage equilibrium (P>0.05), supporting the hypothesis of random association of alleles. These findings are consistent with a pathogen that reproduces both clonally and sexually. Low and insignificant levels of population differentiation were detected, with 90% of the variation occurring among isolates within subpopulations. The high intra-population variation has implications in breeding for resistance to P. fijiensis, as isolates differing in aggressiveness and virulence are likely to exist over small spatial scales. Diverse isolates will be required for resistance screening to ensure selection of banana cultivars with durable resistance to Sigatoka in East Africa

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Population Structure of Pseudocercospora fijiensis in Costa Rica Reveals Shared Haplotype Diversity with Southeast Asian Populations

Cited by 4 publications

References 36 publications

Utilizing Resources and Reducing Emissions through Waste-to-Energy Technologies in Food Processing in the Southeast Asian Agricultural Industry

Utilizing Resources and Reducing Emissions through Waste-to-Energy Technologies in Food Processing in the Southeast Asian Agricultural Industry

Genetic variability of Pseudocercospora fijiensis, the black Sigatoka pathogen of banana (Musa spp.) in Mexico

Genetic Diversity and Mating Type Distribution of Pseudocercospora fijiensis on Banana in Uganda and Tanzania

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