1996
DOI: 10.1095/biolreprod55.2.469
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Porcine Endometrial Glandular Epithelial Cells in Vitro: Transcriptional Activities of the Pregnancy-Associated Genes Encoding Antileukoproteinase and Uteroferrin1

Abstract: The aim of this investigation was to establish a homologous culture system for study of the transcriptional mechanisms underlying endometrial expression of the pregnancy-associated genes encoding antileukoproteinase (ALP), an elastase/cathepsin G protease inhibitor, and uteroferrin (Uf), a transplacental iron transport protein. Glandular epithelial (GE), Luminal epithelial (LE), and stromal (ST) cells were isolated from pig endometrium at Day 12 of pregnancy by differential enzymatic digestion and sieve filtra… Show more

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Cited by 28 publications
(26 citation statements)
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“…These findings largely match the studies made in sows' placentae (Enegar et al 1982, Dantzer & Nielsen 1984, Raub et al 1985, Roberts et al1986, Bazer et al 1991, and in studies which show that the secretion of this protein is a specific genetic expression of the glandular cells (Reed et al 1996).…”
Section: Discus Discus Discus Discus Discussion Sion Sion Sion Sionsupporting
confidence: 87%
“…These findings largely match the studies made in sows' placentae (Enegar et al 1982, Dantzer & Nielsen 1984, Raub et al 1985, Roberts et al1986, Bazer et al 1991, and in studies which show that the secretion of this protein is a specific genetic expression of the glandular cells (Reed et al 1996).…”
Section: Discus Discus Discus Discus Discussion Sion Sion Sion Sionsupporting
confidence: 87%
“…Uterine endometrial GE cells were isolated from day 12 pregnant pig endometrium as previously described (Reed et al 1996). All animal-use procedures were approved by the University of Florida Institutional Animal Care and Use Committee.…”
Section: Cell Culturementioning
confidence: 99%
“…In addition, polarized porcine epithelial cell model systems have been developed for in vitro analysis of pregnancy paradigms, actions of hormones, prostaglandin secretion, and epithelial cell-stromal cell interactions (5,6,24,32).…”
mentioning
confidence: 99%