Porcine Tracheal Goblet Cell Ultrastructure: A Three-Dimensional Reconstruction

Adler, Kenneth B.; Hardwick, D. H.; Craighead, John E.

doi:10.3109/01902148209115817

Cited by 15 publications

(5 citation statements)

References 12 publications

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“…These granules vary in their morphologic and histochemical properties. Three-dimensional reconstruction of mucous goblet cells showed that this variation reflects different stages of mucous cell secretion (Adler et al, 1982). Similarly, the apical surface of mucus cells varies with the stage of secretion.…”

Section: Secretory Cellsmentioning

confidence: 94%

Epithelial Cells of Trachea and Bronchi

Reynolds

Pinkerton

Mariassy

2015

Comparative Biology of the Normal Lung

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Section: Secretory Cellsmentioning

confidence: 94%

Epithelial Cells of Trachea and Bronchi

Reynolds

Pinkerton

Mariassy

2015

Comparative Biology of the Normal Lung

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“…This result indicates a clear difference in airway epithalial tissue organization of indoor reared pigs compared to outdoor reared pigs. The cytoplasm of porcine tracheal goblet cells contains several clusters of mucin granules (Adler et al, 1982); mucin is the protein component of mucus. The airway epithelium is a dynamic tissue capable of undergoing reversible cellular adaptations, including goblet cell hyperplasia, in response to a myriad of stimuli (Chen et al, 1991;Rock et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Airway Metrics, Anatomy and Growth Performance of Pigs Reared Indoors and Outdoors

McClendon

Pettiford

Conklin

et al. 2013

American Journal of Animal and Veterinary Sciences

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While the swine industry has made incredible strides to improve ventilation, air quality in confinement buildings remains an issue. The goal of this study is to characterize correlation between large airway metrics and growth performance in piglets reared indoors and outdoors on pasture. Three experimental trials were conducted: Trial 1 included three breed types that were strictly raised indoors, Tamworth X Berkshire, Berkshire X Berkshire and Hereford X Berkshire (n = 4-5 each). Trials 2 and 3 consisted of animals reared in both environments; trial 2 had 28 pigs (n = 14, indoor and n = 14, outdoor) and trial 3 had 48 pigs (n = 24, indoor and n = 24, outdoor). For trial 3, body weights were recorded weekly for seven weeks to adjust tracheal measure for body size. Total tracheal and lumen diameters were determined for all animals on trial. Histological evaluations were performed to evaluate potential differences among indoor and outdoor pig populations. One-way and two-way Analyses of Variance (ANOVA) were performed and LS means with the PDIFF option was used to separate means as applicable. Animals reared indoor shad significantly larger tracheal diameters and lumens compared to those reared outdoors (p-value<0.05). Outdoor animals had a larger variation of body weights than indoor animals; however, no correlation between tracheal measurements and body weights was present. Histological evaluation of airway sections revealed a 1.4 fold increase in the total population of goblet cells in tracheas of indoor pigs (p-value<0.0152) versus outdoor pig tracheas. Taken together, subtle differences may exist in airways of pigs reared indoors versus outdoors; however, airway distinctions do not appear to effect growth performance of piglets

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“…This cell type is characterized by the presence of numerous large glycoprotein-containing secretory granules. The supranuclear cytoplasm of goblet cells is filled with secretory granules, and the nucleus is located at the cell base (Adler et al 1982;Basbaum et al 1988;Furuya et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Spontaneous remission of edema and regranulation of goblet cells in rat tracheae after capsaicin-induced acute inflammation

2003

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Previous studies have investigated the short-term effect of capsaicin on edema formation and goblet-cell secretion in the trachea. The present study sought to investigate the long-term effect of a high dose of capsaicin (90 micro g/ml/kg), administered intravenously, on changes in the formation of endothelial gaps among venular endothelial cells, mucosal tissue edema and the secretory activity of goblet cells, including the number and size of goblet cells, and the mucus score and secretory ratio of goblet-cell mucus secretion in the trachea of rats. The tracheal whole mounts with silver staining, those stained with chloroacetate esterase reagent and Alcian blue and tracheal tissue sections stained with Alcian blue and periodic acid-Schiff reagent were used for evaluation. Formation of endothelial gaps occurred a few min after administration of capsaicin, and gaps almost closed within 12 min after capsaicin injection. Five min after capsaicin, the leaky blood vessels were numerous and the subepithelial edema ratio (% of length of edema along the inner circumference of tracheal cross section) was found to be 57.8+/-3.0% ( n=6). The number of Alcian blue-positive goblet cells (1,090+/-220 per mm(2) of mucosal surface) was reduced to half the number of goblet cells in the vehicle-treated rats (2,200+/-230). The mucus score of goblet cell secretion was not changed. The secretory ratio was greatly increased. One day after capsaicin, the edema ratio remained large and the number of Alcian blue-positive goblet cells was also small. The mucus score was also not changed. The secretory ratio was still large. On day 3, the edema ratio remained large, but the number of Alcian blue-positive goblet cells was increased to the level of the controls. The mucus score and secretory ratio returned to the control level. On day 5, the edema ratio was greatly decreased, but it was still significantly larger than that of the controls. The mucus score and secretory ratio remained at the baseline level. Seven days after capsaicin, the edema ratio was similar to the controls. The number of goblet cells was even larger than controls. It is concluded that capsaicin-induced acute inflammation in the rat trachea involves formation of endothelial gaps, extensive plasma extravasation and edema formation, and depletion of goblet-cell secretory granules. Spontaneous gradual remission of edema was accompanied by regranulation of goblet cells with gradual mucogenesis for several days.

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Porcine Tracheal Goblet Cell Ultrastructure: A Three-Dimensional Reconstruction

Cited by 15 publications

References 12 publications

Epithelial Cells of Trachea and Bronchi

Epithelial Cells of Trachea and Bronchi

Airway Metrics, Anatomy and Growth Performance of Pigs Reared Indoors and Outdoors

Spontaneous remission of edema and regranulation of goblet cells in rat tracheae after capsaicin-induced acute inflammation

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