Portable Chemiluminescence-Based Lateral Flow Assay Platform for the Detection of Cortisol in Human Serum

Abstract

In this study, we developed the portable chemiluminescence (CL)-based lateral flow assay (LFA) platform for the detection of cortisol in human serum. Cortisol is well-known as a stress hormone due to its high relevancy for human mental and physical health, such as hypertension or depression. To date, a number of optical devices have provided the sensitive determination of levels of analytes. However, this modality type still requires costly optical modules. The developed CL platform is simply composed of two detection modules along with a loading part for the LFA strip. The LFA membrane contains gold nanoparticle probes conjugated with antibodies against cortisol and horseradish peroxidase (HRP), which can also efficiently increase the luminescent signal by providing many areas for anti-cortisol antibody and HRP. The measured voltage signals coming from the photodiode in a CL reader were compared with a standard microplate reader for the evaluation of accuracy. The linear range observed for cortisol was measured to be 0.78–12.5 μg/dL (R2 = 0.99) with a limit of detection (LOD) of 0.342 μg/dL. In addition, the CL-LFA reader showed a high correlation (R2 = 0.96) with the standard cortisol console (COBAS 8000, Roche), suggesting that our developed CL-based LFA platform can be usable in situ.

Toward waterborne protozoa detection using sensing technologies

“…To overcome the short lifetime of the excited state limitation of fluorescence (Berezin and Achilefu, 2010), a luminescence process, in which a photon may be released after any time, was developed (Gaft et al, 2015). Chemiluminescence-based biosensors are another type of optical sensing device (Aboul-Enein et al, 1999), which measure the rate of photon production and generate light through a chemical reaction (Kim et al, 2021). In this optical biosensor, the analyte interacts with the immobilized biomolecule, which is marked with chemiluminescence species.…”

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“…In this work, the LFA membrane consisted of gold nanoparticles conjugated by antibodies against cortisol. Another study reported a LFA platform made of ligand-coated quantum beads and specific nanobodies for early-stage diagnosis of malaria and cardiovascular diseases [ 80 ]. Both examples included smartphone integration for data visualization and analysis.…”

Nanoparticles in the diagnosis and treatment of vascular aging and related diseases

“… 227 , 228 A portable chemiluminescence-based lateral flow assay platform was synthesized by conjugating AuNPs with the anti-cortisol and anti-horseradish peroxidase antibodies, which can be used for serum cortisol detection. 229 Besides, Long et al employed a Cy5-labeled and streptavidin-coated QD probes to detect plasma renin activities, which are tightly associated with hypertension and congestive heart failure. 228 …”