Portable hyperspectral imager for assessment of skin disorders: preliminary measurements

Beach, J. M.; Lanoue, M.; Brabham, Kori; Khoobehi, Bahram

doi:10.1117/12.590938

Cited by 6 publications

(1 citation statement)

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“…[14][15][16][17][18][19] Numerous further reports on spectral imaging that do not cover skin research are available. [20][21][22][23][24] In this work we present a new confocal imaging approach with microscopic spatial resolution to visualize spectral information in tissue. The appropriate image processing steps based on normalized cross-correlation and the derivation of a new quantitative measure is described.…”

Section: Introductionmentioning

confidence: 99%

Evaluation and quantification of spectral information in tissue by confocal microscopy

Maeder¹,

Marquardt²,

Beer³

et al. 2012

J. Biomed. Opt

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Abstract. A confocal imaging and image processing scheme is introduced to visualize and evaluate the spatial distribution of spectral information in tissue. The image data are recorded using a confocal laser-scanning microscope equipped with a detection unit that provides high spectral resolution. The processing scheme is based on spectral data, is less error-prone than intensity-based visualization and evaluation methods, and provides quantitative information on the composition of the sample. The method is tested and validated in the context of the development of dermal drug delivery systems, introducing a quantitative uptake indicator to compare the performances of different delivery systems is introduced. A drug penetration study was performed in vitro. The results show that the method is able to detect, visualize and measure spectral information in tissue. In the penetration study, uptake efficiencies of different experiment setups could be discriminated and quantitatively described. The developed uptake indicator is a step towards a quantitative assessment and, in a more general view apart from pharmaceutical research, provides valuable information on tissue composition. It can potentially be used for clinical in vitro and in vivo applications.

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