Portable light-sheet optofluidic microscopy for 3D fluorescence imaging flow cytometry

Abstract

Imaging flow cytometry (IFC) combines conventional flow cytometry with optical microscopy, allowing for high-throughput, multi-parameter screening of single-cell specimens with morphological and spatial information. However, current 3D IFC systems are...

Is AI essential? Examining the need for deep learning in image-activated sorting of Saccharomyces cerevisiae

“…Flow cytometric systems incorporating other methods for cell imaging have been reported including bright-field, 56–59 2D light-scattering 59–61 and Raman, 15,25 as well as 3D imaging techniques such as tomographic phase microscopy 62 and light-sheet fluorescence microscopy. 63–65 Similar investigations using images generated by a wider range of imaging flow cytometric systems would allow us to again draw more universal deductions about the value of utilizing AI for image classification in IACS overall, as well as provide insights for developing AI-enabled microfluidic cell sorting systems based on imaging modalities that have yet to be extended to cell sorting.…”

Structured-light-sheet imaging in an integrated optofluidic platform

“…By combining LSFM with microfluidics 8 the samples can be automatically flown across the illumination region, thus overcoming limitations related to the sample mounting stages. This approach has been implemented by exploiting tubes, 9 capillaries 10 and microfluidic chips [11][12][13][14][15] for the sample delivery. The system integration has been further increased by combining optical and fluidic elements on the same platform, by adding inclined micromirrors to reflect the incoming light sheet and project it orthogonally onto the sample 4,16,17 or by embedding microlenses in the microfluidic chip to generate the light sheet.…”

An optimized PDMS microfluidic device for ultra-fast and high-throughput imaging flow cytometry

“…9 These circuitous methods always require some data processing to reflect the morphological characteristics of cells. Alternatively, imaging flow cytometry (IFC) can perform these processes directly, 10 such as intercellular or detail analysis by fluorescence imaging, 11,12 Raman spectroscopy, 13 and visualization of the 3D structural shapes of cells by tomographic imaging, 14 light sheet scanning 15 or rotating cells. 16 For most IFC studies, the high velocity of cells can enhance the efficiency and throughput of detection, but high velocity of moving objects means shorter exposure time of the camera.…”