Portable microfluidic and smartphone-based devices for monitoring of cardiovascular diseases at the point of care

Hu, Jie; Cui, Xingye; Gong, Yan; Gao, Bin; Wen, Ting; Lu, Tian Jian; Xu, Feng

doi:10.1016/j.biotechadv.2016.02.008

Cited by 138 publications

(69 citation statements)

References 118 publications

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“…CASA and a smartphone-based motility analysis system can be used to determine sperm trajectory and motion under optical microscopic observation131415. Because mammalian sperm motility is expressed by motor protein function using adenosine triphosphate (ATP) catalysis, ATP shuttle system, and ATP production system1617, analyses of sperm cell chemical reactions may also be used as potential indicators of sperm motility17.…”

mentioning

confidence: 99%

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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Mammalian sperm motility has traditionally been analyzed to determine fertility using computer-assisted semen analysis (CASA) systems. To develop low-cost and robust male fertility diagnostics, we created a paper-based MTT assay and used it to estimate motile sperm concentration. When porcine sperm motility was inhibited using sperm enzyme inhibitors for sperm enzymes related to mitochondrial activity and glycolysis, we simultaneously recorded sperm motility and enzymatic reactivity using a portable motility analysis system (iSperm) and a paper-based MTT assay, respectively. When using our paper-based MTT-assay, we calculated the area mean value signal intensity (AMV) to evaluate enzymatic reactivity. Both sperm motility and AMV decreased following treatment with iodoacetamide (IODO) and 3-bromopyruvic acid (3BP), both of which are inhibitors of glycolytic enzymes including glyceraldehyde-3-phosphate dehydrogenase (GAPDH). We found a correlation between recorded motility using iSperm and AMV from our paper-based assay (P < 0.05), suggesting that a sperm-related enzymatic reaction is involved in sperm motility. Under this protocol, MTT reduction was coupled with catalysis of GAPDH and was promoted by electron transfer from NADH. Based on this inhibitor study, sperm motility can be estimated using our paper-based MTT-assay.

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mentioning

confidence: 99%

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Matsuura

Huang

Chen

et al. 2017

Sci Rep

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“…Although broadband penetration is low in developing countries, remaining below 10 % in South Africa, smartphones have penetrated approximately 40 % of these areas [15,16]. Hence, based on information and communication technologies (ICT) infrastructure conditions in developing countries, portable smartphones are the best suited terminals for combinations of diagnostics and medical instruments, such as microscopes and electrocardiograms (ECG) [17,18]. Accordingly, smartphones have been used individually and with other components or devices to monitor cardiovascular disease [17].…”

Section: Smartphone-based Optical Systemsmentioning

confidence: 99%

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

Matsuura¹,

Nishina²,

Asano³

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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“…Some complicated assays such as enzyme-linked immunosorbent assay (ELISA) (Chen et al 2014a;Wang et al 2011) and polymerase chain reaction (PCR) Liao et al 2016;Stedtfeld et al 2012) were successfully implemented with the MS 2 systems. Furthermore, MS 2 systems offers advantages in test speed, self-containment and sample to result assay operation, which are required for in field test and point of care (PoC) diagnosis (D'Ambrosio et al 2015;Hu et al 2016;Laksanasopin et al 2015;Mudanyali et al 2012). Those MS 2 applications integrated sophisticated assay control accessories and sample-chip-phone interfaces, which demonstrate the potential of MS 2 to enable high-level biological applications.…”

Section: Introductionmentioning

confidence: 99%

Mkit: A cell migration assay based on microfluidic device and smartphone

Yang

Peretz‐Soroka

et al. 2018

Biosensors and Bioelectronics

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Mobile sensing based on the integration of microfluidic device and smartphone, so-called MS 2 technology, has enabled many applications over recent years, and continues to stimulate growing interest in both research communities and industries. In particular, it has been envisioned that MS 2 technology can be developed for various cell functional assays to enable basic research and clinical applications. Toward this direction, in this paper, we describe the development of a MS 2 -based cell functional assay for testing cell migration (the M kit ). The system is constructed as an integrated test kit, which includes microfluidic chips, a smartphone-based imaging platform, the phone apps for image capturing and data analysis, and a set of reagent and accessories for performing the cell migration assay. We demonstrated that the M kit can effectively measure purified neutrophil and cancer cell chemotaxis. Furthermore, neutrophil chemotaxis can be tested from a drop of whole blood using the M kit with red blood cell (RBC) lysis. The effects of chemoattractant dose and gradient profile on neutrophil chemotaxis were also tested using the M kit . In addition to research applications, we demonstrated the effective use of the M kit for on-site test at the hospital and for testing clinical samples from chronic obstructive pulmonary disease patient. Thus, this developed M kit provides an easy and integrated experimental platform for cell migration related research and potential medical diagnostic applications.

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Portable microfluidic and smartphone-based devices for monitoring of cardiovascular diseases at the point of care

Cited by 138 publications

References 118 publications

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Relationship between Porcine Sperm Motility and Sperm Enzymatic Activity using Paper-based Devices

Recent advances in microfluidic diagnostic and treatment systems for assisted reproductive technologies in developing countries

Mkit: A cell migration assay based on microfluidic device and smartphone

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