Portable Polymerase Chain Reaction (PCR): Thermal ramping rate performance evaluation

Chong, K. S.; Beng, Gan Kok

doi:10.1109/iecbes.2016.7843490

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Thermal conductivity is one of the crucial factors in the conduction of material. Therefore, a good thermal conductivity value determines the quality of a good conductor material [19]. It was observed that silver had the highest thermal conductivity value of 429 W/mK.…”

Section: Resultsmentioning

confidence: 99%

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

Lischer¹,

Putra²,

Sahlan³

et al. 2021

JMechE

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Medical diagnosis is the initial stage in identifying a person's condition, disease or injury from its signs and symptoms. The diagnostic method is carried out quantitatively by using a diagnostic kit which measures data such as blood pressure, heart rate frequency and blood cell concentration. These diagnostic kits are available in their respective capabilities and their activities require medical facilities and logistical readiness to function. Furthermore, Indonesia's geographical condition which consists of many islands and mountains causes uneven distribution of health facilities and laboratories in each region. Therefore, resulting in problems such as inadequate access and availability of these diagnostic kit in each region. Presently, one of the most widely used diagnostic methods is the Polymerase Chain Reaction (PCR) which allows the amplification of specific fragments from complex DNA. In PCR, only a small amount of DNA is needed to produce enough replication copies which were further analyzed by microscopic examination. This process begins with thermal cycling, which is the reactant's exposure to the heating cycle and repetitive repairs to produce reactions to different temperatures. This study aims to examine the material used for thermal cyclers which is an essential aspect of the heat transfer needed by the PCR process. In this study, heat transfer from several materials were simulated and analyzed by COMSOL Multiphysics 5.3 Software. The following results were obtained from the simulation: the saturation time for heating aluminum, copper and nickel were 29,37 and 51 seconds, respectively. Meanwhile, the cooling time was 26, 35 and 55 seconds, respectively. In addition, the saturation time for heating and cooling silver and Polydimethylsiloxane (PDMS) were 26 and 1480 seconds, respectively.

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Section: Resultsmentioning

confidence: 99%

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

Lischer¹,

Putra²,

Sahlan³

et al. 2021

JMechE

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show abstract

“…Therefore, one interest in this technology is engineering the thermal process during the amplification 5) . A recent development in microcontroller enables us to realize simple thermal management hence adding the portability of the device [6][7][8] . Additionally, the energy management was proven to be handle by batteries to make it more portable [9][10][11] .…”

Section: Introductionmentioning

confidence: 99%

Heating Characterization of Low Energy Consumption Lab-on-a-Chip

Irwansyah¹,

Juana²,

Whulanza³

et al. 2021

Evergreen

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The ultimate goal of this study is to develop an effective and user-friendly form of thermal cycler to perform Polymerase Chain Reaction (PCR). This paper research the ability to manufacture a thermal cycler using conventional methods and readily available components to achieve a significant performance result with low production cost compared with commercially available counterparts. Commonly, thermal cyclers use an aluminum heating block as a heating vessel for samples, to achieve fast temperature changes and uniformity, but Polydimethylsiloxanes (PDMS) lab-on-a-chip (LoC) is utilized in contrast to silver as a heating vessel in this study to achieve biocompatibility with various samples. The raspberry-Pi pocket computer was used to achieve the necessary control with room for future capabilities addition and improvement. Characterization of the heating and PCR processes was done to understand the heat transfer phenomenon and thermal cycling speed in PDMS environment. This paper also includes analysis of the cycle speed corresponding to the control parameters of the manufactured thermal cycler. Ultimately, the characterization and analysis results will show the achieved performance capabilities of the thermal cycler.

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Microwave Heating System for the LAMP Technique based DNA Amplification Process

Chuenarom

Silapunt

2018

2018 15th International Conference on Electrical Engineering/Electronics, Computer, Telecommunications and Information Technolo

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Portable Polymerase Chain Reaction (PCR): Thermal ramping rate performance evaluation

Cited by 3 publications

References 8 publications

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

Heat Transfer Simulation of Various Material for Polymerase Chain Reaction Thermal Cycler

Heating Characterization of Low Energy Consumption Lab-on-a-Chip

Microwave Heating System for the LAMP Technique based DNA Amplification Process

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