Portable potentiostatic sensor integrated with neopterin-imprinted poly(ethylene- co -vinyl alcohol)-based electrode

Abstract

Neopterin is a catabolic product of guanosine triphosphate, a purine nucleotide. Measuring neopterin concentrations in biological fluids such as urine provides information about cellular immune activation in humans under control of T helper cells. A high neopterin concentration in bodily fluids, including serum and urine, indicates cellular immunity activation, which is associated with oxidative stress. In this work, neopterin is the target molecule and imprinted onto poly(ethylene-co-vinyl alcohol) via solvent evaporation. The template molecules on the thin film are then removed, and the membrane is used as a sensing element for electrochemical urinalysis. Poly(ethylene-co-vinyl alcohol) containing 27 mol% ethylene had high imprinting effectiveness and may be integrated with the proposed portable biosensor. In random urine analysis, the cyclic voltammetry measurements of neopterin with an additional recovery method achieved >95% recovery for the neopterin concentration of 15 ng/mL.

Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

“…A biosensor designed by imprinting neopterin onto poly(ethylene- co -vinyl alcohol) as template molecules was developed by Huang et al After imprinting, the template is removed, and the membrane can be used as a sensing element for electrochemical analysis of urine. The LOD for the biosensor was reported to be as low as 0.025 pg/mL [ 37 ]. Endocrine-disrupting chemicals such as diethylstilbestrol and bisphenol can disrupt naturally occurring endocrine control causing diseases and disorders such as cancer and epigenetic dysfunction.…”

ICITST-2013: Keynote speaker 3: Blurred nets: Disruption and the risk to future ‘Internet of Things’ (IoT) architectures

“…Besides, the low-cost and portable C. Y. Huang potentiostats [5]- [7] suitable for home care applications were also proposed. Due to their emphasis merely upon the integration of potentiostats and sensors, they didn't further develop the application of potentiostatic biosensors.…”