Portable semen analyzer for automated, rapid measurement of sperm motility and concentration

Kanakasabapathy, Manoj Kumar; Thirumalaraju, Prudhvi; Yogesh, Vinish; Natarajan, V.; Bormann, Charles L.; Shafiee, Hadi

doi:10.1016/j.fertnstert.2017.07.235

Cited by 2 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, portable and relatively inexpensive units have become available for the measurement of concentration and motility of spermatozoa from a range of domestic animals (Buss, Aurich, & Aurich, ; Kanakasabapathy et al, ; Kjelland, Stroud, Ayliffe, & Dittami, ). One of these portable devices is iSperm ® (Aidmics Biotechnology), a CASA system installed on an iPad (Apple Inc.) equipped with an optical device with integrated heater and software which functions on the Apple iOS (up iSperm App).…”

Section: Introductionmentioning

confidence: 99%

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration

Dini

Troch

Lemahieu

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

The objective of this study was to evaluate the accuracy of a novel, portable device (iSperm® Equine for assessing concentration and motility of stallion semen). In the first experiment, semen concentration was determined by the iSperm® Equine (Aidmics Biotechnology), Androvision® (Minitube) and NucleoCounter® SP‐100™ (ChemoMetec). The total motility and progressive motility were determined by the iSperm® Equine and the Androvision® using the manufacturer's guidelines. Frozen/thawed semen samples (n = 33) at various dilutions were analysed for concentration and motility with the above‐mentioned devices. There was a significant correlation between the concentrations measured with iSperm® and NucleoCounter® at all the measured dilutions. Moreover, <10% difference in concentrations was observed between the iSperm® and NucleoCounter® using the Bland–Altman test. There was also a significant correlation between iSperm® and Androvision® for total and progressive motility. In the second experiment, the parameters used in the Androvision® were modified to match those of the iSperm®. Total motility and progressive motility of frozen/thawed semen samples (n = 10) were determined, and the similarity between the Androvision® and iSperm® was confirmed by correlation studies and Bland–Altman test. The results of these experiments demonstrate that the iSperm® offers a reliable and practical alternative for the semi‐automated measurement of concentration and motility of stallion semen in the field. The iSperm® enables the practitioner to obtain objective and repeatable measurements on a variety of semen types (fresh, cooled and frozen) in the field at the time of insemination and thus acquire more insight into the quantity and quality of the provided insemination doses. This mare‐side diagnostic tool may help practitioners in identifying presumed subfertility problems more rapidly and act accordingly.

show abstract

Section: Introductionmentioning

confidence: 99%

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration

Dini

Troch

Lemahieu

et al. 2019

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

Automated smartphone-based system for measuring sperm viability, DNA fragmentation, and hyaluronic binding assay score

et al. 2019

View full text Add to dashboard Cite

The fundamental test for male infertility, semen analysis, is mostly a manually performed subjective and time-consuming process and the use of automated systems has been cost prohibitive. We have previously developed an inexpensive smartphone-based system for at-home male infertility screening through automatic and rapid measurement of sperm concentration and motility. Here, we assessed the feasibility of using a similar smartphone-based system for laboratory use in measuring: a) Hyaluronan Binding Assay (HBA) score, a quantitative score describing the sperm maturity and fertilization potential in a semen sample, b) sperm viability, which assesses sperm membrane integrity, and c) sperm DNA fragmentation that assesses the degree of DNA damage. There was good correlation between the manual analysis and smartphone-based analysis for the HBA score when the device was tested with 31 fresh, unprocessed human semen samples. The smartphone-based approach performed with an accuracy of 87% in sperm classification when the HBA score was set at manufacturer’s threshold of 80. Similarly, the sperm viability and DNA fragmentation tests were also shown to be compatible with the smartphone-based system when tested with 102 and 47 human semen samples, respectively.

show abstract

Portable semen analyzer for automated, rapid measurement of sperm motility and concentration

Cited by 2 publications

References 0 publications

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration

Automated smartphone-based system for measuring sperm viability, DNA fragmentation, and hyaluronic binding assay score

Contact Info

Product

Resources

About

Portable semen analyzer for automated, rapid measurement of sperm motility and concentration

Cited by 2 publications

References 0 publications

Validation of a portable device (iSperm®) for the assessment of stallion sperm motility and concentration

Validation of a portable device (iSperm®) for the assessment of stallion sperm motility and concentration

Automated smartphone-based system for measuring sperm viability, DNA fragmentation, and hyaluronic binding assay score

Contact Info

Product

Resources

About

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration

Validation of a portable device (iSperm^®) for the assessment of stallion sperm motility and concentration