Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities

Silva, José Dayvid Ferreira da; Silva, Suéllen Pedrosa da; Silva, Pollyanna Michelle da; Vieira, Amanda Mota; Araújo, Larissa Cardoso Corrêa de; Lima, Thâmarah de Albuquerque; Oliveira, Ana Patrícia Silva de; Carvalho, Lidiane Vasconcelos do Nascimento; Pitta, Maíra Galdino da Rocha; Rêgo, Moacyr Jesus Barreto de Melo; Pinheiro, Irapuan Oliveira; Zingali, Russolina B.; Cavalcanti, María Auxiliadora de Queiroz; Napoleão, Thiago Henrique; Paiva, Patrícia Maria Guedes

doi:10.1016/j.ijbiomac.2018.12.188

Cited by 49 publications

(16 citation statements)

References 32 publications

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“…The antibacterial activity of lectins was attributed to their interaction with the glycans of the bacterial cell wall [49]. In addition, antibacterial lectins can promote protein leakage and the formation of pores in the cell wall [50]. As shown in Figure 12, S. aureus , E. coli, and B. subtilis were capable of being inhibited by the lectin.…”

Section: Resultsmentioning

confidence: 99%

Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds

Jiang

Wang

et al. 2019

Polymers

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A two-step method based on an aqueous two-phase system and Sephadex G-75 was used to separate and purify lectin from the seeds of the Zihua snap bean. The preliminary properties and bioactivity of the Zihua snap bean lectin were characterized by different instrumental methods, such as sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS–PAGE), liquid chromatography-nano electrospray ionization mass spectrometry (Nano LC-ESI-MS/MS), and Fourier transform infrared spectroscopy (FTIR). The hemagglutinating activity of the Zihua snap bean lectin could not be inhibited by glucose, N-acetyl-d-glucosamine, d-galactose, N-acetyl-d-galactosamine, fructose, sucrose, d-maltose, d-trehalose, and lactose. It was found that the hemagglutinating activity of the lectin showed strong dependence on Mn2+ and Ca2+. The thermal and pH stability of the Zihua snap bean lectin was studied by FTIR and fluorescence spectroscopy. Relatively good stability was observed when the temperature was not higher than 70 °C, as well as in the pH range of 2.0 to 10.0. Digestive stability in vitro was investigated. The untreated lectin was relatively stable to pepsin and trypsin activity, but heat treatment could significantly reduce the digestive stability in vitro. Moreover, the lectin showed an inhibitory effect on the tested bacteria (Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Bacillus subtilis (B. subtilis)), and it also showed a certain inhibitory effect on the growth of Phytophthora infestans (P. infestans) at higher concentrations.

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Section: Resultsmentioning

confidence: 99%

Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds

Jiang

Wang

et al. 2019

Polymers

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“…Many ethnobotanical studies have reported its use against multiple diseases and ailments (Ahmad and Beg, 2001;Bosi et al, 2009;Nedelcheva, 2013;Sultana and Raheman, 2013;Hwess et al, 2018;Chaachouay et al, 2019;Manzanero-Medina et al, 2020;Nanagulyan et al, 2020). Recent pharmacological studies also suggest its important medicinal benefits against a number of diseases such as cancer, diabetes, and viral, bacterial, and fungal infections (Dong et al, 2010;Ye et al, 2015;Jin et al, 2017;Park and Han, 2018;Zhao et al, 2018;da Silva et al, 2019;Li et al, 2019;El-Desouky, 2021;Park et al, 2021;Tleubayeva et al, 2021). These results of pharmacological studies provided important evidence toward the potential of purslane in drug development against diseases.…”

Section: Medicinal Potentialmentioning

confidence: 95%

Improvement of a Traditional Orphan Food Crop, Portulaca oleracea L. (Purslane) Using Genomics for Sustainable Food Security and Climate-Resilient Agriculture

Kumar

Sreedharan

Singh

et al. 2021

Front. Sustain. Food Syst.

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Purslane (Portulaca oleracea L.) is a popular orphan crop used for its nutritional properties in various parts of the world. It is considered one of the richest terrestrial sources of omega-3 and omega-6-fatty acids (ω-3 and 6-FAs) suggesting its importance for human health. This ethnomedicinal plant is also an important part of traditional healing systems among the indigenous people. Many studies have indicated its tolerance against multiple stresses and found that it easily grows in a range of environmental gradients. It has also been considered one of the important biosaline crops for the future. Despite its huge nutritional, economic, and medicinal importance, it remains neglected to date. Most of the studies on purslane were focused on its ethnomedicinal, phytochemical, pharmacological, and stress-tolerance properties. Only a few studies have attempted genetic dissection of the traits governing these traits. Purslane being an important traditional food crop across the globe can be valorized for a sustainable food security in the future. Therefore, this review is an attempt to highlight the distribution, domestication, and cultivation of purslane and its importance as an important stress-tolerant food and a biosaline crop. Furthermore, identification of genes and their functions governing important traits and its potential for improvement using genomics tools for smart and biosaline agriculture has been discussed.

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“…Lectins are a heterogeneous group of proteins capable of reversibly and specific binding to sugars [141]. The Mannosebinding lectin (MBL) participates strongly in the innate immune system before specific antibody responses.…”

Section: Treatment Strategiesmentioning

confidence: 99%

SARS-CoV, MERS-CoV and SARS-CoV-2 infections in pregnancy and fetal development

Silva

Costa

et al. 2020

Journal of Gynecology Obstetrics and Human Reproduction

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Recently, in China, in 2019, a new type of disease has arisen caused by a new strain of coronavirus, the SARS-CoV-2 virus, considered extremely worrying due to its high infectivity power and the easy ability to spread geographically. For patients in general, the clinical features resulting from respiratory syndromes can trigger an asymptomatic condition. However, 25 % of patients infected by SARS-CoV-2 can progress to severity. Pregnant women are an unknown field in this complex process, and although they have symptoms similar to non-pregnant women, some points should be considered, such as complications during pregnancy and postpartum. Thus, the aim of this study was to understand the consequences of pregnancy and fetal development, caused by infections by the SARS-CoV, MERS-CoV and SARS-CoV-2 viruses. Among the aforementioned infections, MERS-CoV seems to be the most dangerous for newborns, inducing high blood pressure, pre-eclampsia, pneumonia, acute renal failure, and multiple organ failure in mother. This also causes a higher occurrence of emergency cesarean deliveries and premature births, in addition, some deaths of mothers and fetuses were recorded. Meanwhile, SARS-CoV and SARS-CoV-2 appear to have less severe symptoms. Furthermore, although a study found the ACE2 receptor, used by SARS-CoV-2, widely distributed in specific cell types of the maternal-fetal interface, there is no evidence of vertical transmission for any of the coronaviruses. Thus, the limited reported obstetric cases alert to the need for advanced life support for pregnant women infected with coronaviruses and to the need for further investigation for application in clinical practice.

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Portulaca elatior root contains a trehalose-binding lectin with antibacterial and antifungal activities

Cited by 49 publications

References 32 publications

Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds

Two-Step Isolation, Purification, and Characterization of Lectin from Zihua Snap Bean (Phaseolus vulgaris) Seeds

Improvement of a Traditional Orphan Food Crop, Portulaca oleracea L. (Purslane) Using Genomics for Sustainable Food Security and Climate-Resilient Agriculture

SARS-CoV, MERS-CoV and SARS-CoV-2 infections in pregnancy and fetal development

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