Positive Charged Polymer as a Probe for DNA Determination by Resonance Light Scattering

Abstract

Electrostatic interactions of calf thymus DNA (ct-DNA) with a positive charged polymer were investigated based on measurements of enhanced resonance light scattering (RLS) signals, and were proved by scanning electron microscopy (SEM). The optimal pH value was 7.0 in a phosphate-buffered saline solution (PBS) and the maximum RLS peak located at 344.8 nm. The RLS signal enhancement (DIRLS) could be linearly correlated with the concentration of ct-DNA in the range of 0.0900 to 2.70 mg mL -1 , and the detection limit (S/N = 3) was 17.0 ng mL -1 . The binding ratio and the binding constant between DNA and polymer were also provided.

Study on the interaction between palladium(II)-chlorpromazine hydrochloride and sodium tungstate by the resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectra and its analytical application

“…They have been widely applied to the study and determination of nucleic acids [14,15], proteins [16][17][18], polysaccharides [19], and drugs [20][21][22]. In our work, we find that many of them can produce strong light scattering at double and half wavelength of the incident light.…”

Synthesis of polycation with gemini structure and detection of DNA by resonance light scattering

“…However, these small molecule light scattering probes have disadvantages of high material cost or cellular toxicity more or less, which would affect their application in life science. In contrast, cationic polymers are displaying some potential for DNA quantitative analysis, but very limited researches have been reported [36,37].…”

Determination of Promethazine Hydrochloride with Palladium(II) and Na2WO4by Resonance Rayleigh Scattering and Resonance Nonlinear Scattering Spectra

“…Because of their high sensitivities, accuracy, and low outlay, new analytical techniques of RRS, SOS, and FDS have been applied in the determination of nucleic acids, [23] proteins, [24][25][26][27][28] and some drugs. [29][30][31][32][33][34][35][36][37] Our experiments found that at room temperature and pH 4.6-5.3 Britton-Robinson (BR) buffer medium, Pd(II) reacted with PMZ to form 1:2 cationic chelate [Pd(PMZ) 2 ] 2þ , which further reacted with Na 2 WO 4 to form a 1:1 ternary ion-association complex ([Pd(PMZ) 2 ] Á (WO 4 )) and RRS, SOS, FDS were increased rapidly.…”