Positive effect of butylated hydroxytoluene (BHT) on the quality of cryopreserved cat spermatozoa

Jara, Belén; Merino, O.; Sánchez, Raúl; Risopatrón, Jennie

doi:10.1016/j.cryobiol.2019.05.003

Cited by 11 publications

(7 citation statements)

References 52 publications

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“…These cells are particularly vulnerable to this process because they are richly endowed with polyunsaturated fatty acids, the double bonds of which facilitate the hydrogen abstraction process that initiates the peroxidation cascade [8]. The causative links between oxidative stress, lipid peroxidation and sperm function have subsequently been confirmed many times by independent groups for human spermatozoa [9][10][11][12][13] and the spermatozoa of every other mammalian species examined including the bull [14], stallion [15,16], pig [17,18], dog [19], cat [20], etc. This fundamental concept, pioneered by such luminaries as Jones and Mann [21], Gagnon [22] and Storey [6] is fundamental to our understanding of the aetiology of defective sperm function.…”

Section: Introductionmentioning

confidence: 99%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

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This article addresses the importance of oxidative processes in both the generation of functional gametes and the aetiology of defective sperm function. Functionally, sperm capacitation is recognized as a redox-regulated process, wherein a low level of reactive oxygen species (ROS) generation is intimately involved in driving such events as the stimulation of tyrosine phosphorylation, the facilitation of cholesterol efflux and the promotion of cAMP generation. However, the continuous generation of ROS ultimately creates problems for spermatozoa because their unique physical architecture and unusual biochemical composition means that they are vulnerable to oxidative stress. As a consequence, they are heavily dependent on the antioxidant protection afforded by the fluids in the male and female reproductive tracts and, during the precarious process of insemination, seminal plasma. If this antioxidant protection should be compromised for any reason, then the spermatozoa experience pathological oxidative damage. In addition, situations may prevail that cause the spermatozoa to become exposed to high levels of ROS emanating either from other cells in the immediate vicinity (particularly neutrophils) or from the spermatozoa themselves. The environmental and lifestyle factors that promote ROS generation by the spermatozoa are reviewed in this article, as are the techniques that might be used in a diagnostic context to identify patients whose reproductive capacity is under oxidative threat. Understanding the strengths and weaknesses of ROS-monitoring methodologies is critical if we are to effectively identify those patients for whom treatment with antioxidants might be considered a rational management strategy.

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Section: Introductionmentioning

confidence: 99%

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

2020

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“…As a promising antioxidant, BHT has been discussed exclusively in nearly six thousand publications ( Yehye et al, 2015 ). Thus far, this lipid-soluble phenolic compound has been confirmed positive effects on cryopreserved sperm ( Jara et al, 2019 ; De Andrade et al, 2023 ). Specifically, BHT readily penetrate the sperm membrane, enhancing its fluidity and influencing the membrane phase transition.…”

Section: Discussionmentioning

confidence: 81%

“…Thereinto, butylated hydroxytoluene (BHT) is being investigated as a component to cryomedia in different animal species, including goat ( Memon et al, 2011 ), human ( Merino et al, 2015 ), boar ( Sus scrofa ) ( Trzcinska et al, 2015 ), cat ( Jara et al, 2019 ), and buffalo ( Nain et al, 2022 ), because of its safety and notable anti-oxidative potential. BHT is a synthetic analogue of vitamin E, characterized with the low polarity and high lipid solubility ( Yehye et al, 2015 ; Achar et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The antioxidant effects of butylated hydroxytoluene on cryopreserved goat sperm from a proteomic perspective

Li,

Allai,

Liang

et al. 2024

PeerJ

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At present, there are few reports about the proteomics changes provoked by butylated hydroxytoluene (BHT) supplementation on cryopreserved semen in mammals. Thus, we aimed to evaluate the effects of different concentrations of BHT on goat sperm and to investigate the proteomics changes of adding BHT to cryopreserved goat (Capra hircus) sperm. Firstly, semen samples were collected from four goats, and frozen in the basic extenders containing different concentrations of BHT (0.5 mM, 1.0 mM, 2.0 mM) and a control without BHT, respectively. After thawing, the protective effects of dose-dependent replenished BHT to the freezing medium on post-thaw sperm motility, integrities of plasma membrane and acrosome, reactive oxygen species levels were confirmed, with 0.5 mM BHT being the best (B group) as compared to the control (without BHT, C group). Afterwards, TMT-based quantitative proteomic technique was performed to profile proteome of the goat sperm between C group and B group. Parallel reaction monitoring was used to confirm reliability of the data. Overall, 2,476 proteins were identified and quantified via this approach. Comparing the C and B groups directly (C vs. B), there were 17 differentially abundant proteins (DAPs) po-tentially associated with sperm characteristics and functions were identified, wherein three were upregulated and 14 were downregulated, respectively. GO annotation analysis demonstrated the potential involvement of the identified DAPs in metabolic process, multi-organism process, reproduction, reproductive process, and cellular process. KEGG enrichment analysis further indicated their potential roles in renin-angiotensin system and glutathione metabolism pathways. Together, this novel study clearly shows that BHT can effectively improve quality parameters and fertility potential of post-thawed goat sperm at the optimal concentration, and its cryoprotection may be realized through regulation of sperm metabolism and antioxidative capability from the perspective of sperm proteomic modification.

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“…The inclusion of quercetin or BHT in the semen with a freezing egg yolk-based extender was tested for sperm motility and viability of either cooled or frozen-thawed ram semen. Although quercetin has been reported to improve postthawed sperm parameters of bulls (Avdatek et al, 2018;Tvrdá, Tušimová, Kováčik, Paál, & Lukáč, 2016), horses (Seifi-Jamadi et al, 2016), roosters (Appiah et al, 2020), rams (Banday, Lone, Rasool, Rashid & Shikari, 2017), buffalo (El-Khawagah, Kandiel, & Samir, 2020), and dogs (Kawasaki et al, 2020), and the inclusion of BHT improved cryopreserved sperm parameters in cats (Jara et al, 2019), dogs (Sun et al, 2020), boars (Trzcińska, Bryła, Gajda, & Gogol, 2015), goats (Memon et al, 2011), and bulls (Shoae, & Zamiri, 2008), in the present study, these substances were not able to produce better sperm motility parameters or PMI in ram semen stored at 5 °C or frozen-thawed. It is well known that sperm motility parameters and fertility are negatively affected by the overproduction of ROS and membrane lipid peroxidation, and findings from previous studies demonstrated that these antioxidants prevent sperm damage caused by oxidative stress (Guthrie & Welch, 2012).…”

Section: Resultsmentioning

confidence: 99%

“…Quercetin scavenges ROS and hydroxyl radicals (Boots, Haenen & Bast, 2008), and has been suggested as a potent antioxidant for the cryopreservation of semen from stallions, rats, rooster, and dogs (Gibb et al, 2013;Ben et al, 2011;Appiah et al, 2020;Kawasaki et al, 2020). Another antioxidant added to semen extenders to minimize sperm damage caused by oxidative stress and cold shock is butylated hydroxytoluene (BHT) (Jara, Merino, Sánchez, & Risopatrón, 2019;Merino et al, 2015). Butylated hydroxytoluene is a synthetic analog of vitamin E that reacts with peroxy radicals to transform them into hydroperoxides, interrupting the oxidative process (Seifi-Jamadi, Kohram, Zareh-Shahne, Dehghanizadeh, & Ahmad, 2016).…”

Section: Introductionmentioning

confidence: 99%

Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality

Canuto

Segabinazzi

Araújo

et al. 2022

Semina: Ciênc. Agrár.

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Cooling and freezing processes cause physical and chemical damage to sperm by cold shock and oxidative stress. This study aimed to evaluate the effect of two antioxidants on sperm parameters of cooled and frozen-thawed ram semen diluted in an egg yolk-based extender. Semen was collected from 30 rams and processed in two consecutive experiments to test the inclusion of different concentrations of quercetin and butylated hydroxytoluene (BHT) in an egg yolk-based semen extender. Dimethyl sulfoxide (DMSO) was added as a solvent to the semen extender in a ratio of 1 mL DMSO for 90 mg of quercetin and 1 mL DMSO for 880 mg of BHT. After collection, semen was diluted at 200 × 106 motile sperm/mL (control) and split into different groups in each experiment. In experiment 1, semen was diluted with the extender containing quercetin (Q5, 5 μg/mL; Q10, 10 μg/mL; Q15, 15 μg/mL) or DMSO alone (DMSO1, 0.055 μL DMSO per mL; DMSO2, 0.165 μL DMSO per mL). In experiment 2, semen was diluted with the extender with BHT (BHT1, 0.5 μg/mL; BHT2, 1 μg/mL; BHT3, 1.5 μg/mL) or DMSO alone (DMSO3, 0.375 μL DMSO per mL; DMSO4, 1.125 μL DMSO per mL). After dilution, the semen was divided into two aliquots. Treated ram sperm samples were also subjected to different storage methods. The first set of samples was cooled at 5 °C for 24 h, whereas the second set of samples was frozen-thawed. Sperm motility parameters and plasma membrane integrity (PMI) were evaluated immediately after dilution (0h) and 24 h after cooling and in the frozen-thawed samples via computer-assisted sperm analysis and epifluorescence microscopy, respectively. The inclusion of quercetin or BHT did not affect sperm motility parameters or PMI of fresh, cooled, or frozen-thawed sperm in this study (P < 0.05). However, further studies are needed to test the effects of these antioxidants on the fertility of cryopreserved ram semen.

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Positive effect of butylated hydroxytoluene (BHT) on the quality of cryopreserved cat spermatozoa

Cited by 11 publications

References 52 publications

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

The Importance of Oxidative Stress in Determining the Functionality of Mammalian Spermatozoa: A Two-Edged Sword

The antioxidant effects of butylated hydroxytoluene on cryopreserved goat sperm from a proteomic perspective

Effect of quercetin or butylated hydroxytoluene on cooled or frozen-thawed ram sperm quality

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