Positive hyaluronan/PEI/DNA complexes as a target-specific intracellular delivery to malignant breast cancer

Sun, Xuejian; Ma, Ping; Cao, Xinke; Li, Ning; Tian, Yun; Chang-shan, Ren

doi:10.1080/10717540903059549

Cited by 19 publications

(17 citation statements)

References 24 publications

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“…Transfection efficiencies for MCF-7 and 4T1 breast cancer cells have been reported using cationic lipids [32], chitosan cationic polymer [33,34], PEI [35,36], and gas plasma [37]. In the present study, the highest transfection efficiency in MCF-7 cells using both luciferase and bgalactosidase genes in the presence and absence of serum was seen with Arrest-In and jetPEI.…”

Section: Discussionsupporting

confidence: 53%

Comparison of Transfection Efficiency of Nonviral Gene Transfer Reagents

2010

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This study compared six commercially available reagents (Arrest-In, ExpressFect, FuGENE HD, jetPEI, Lipofectamine 2000, and SuperFect) for gene transfection. We examined the efficiency and cytotoxicity using nine different cell lines (MC3T3-E1 mouse preosteoblasts, PT-30 human epithelial precancer cells, C3H10T1/2 mouse stem cells, MCF-7 human breast cancer cells, HeLa human cervical cancer, C2C12 mouse myoblasts, Hep G2 human hepatocellular carcinoma, 4T1 mouse mammary carcinoma, and HCT116 human colorectal carcinoma), and primary cells (HEKn human epidermal keratinocytes) with two different plasmid DNAs encoding luciferase or β-galactosidase in the presence or absence of serum. Maximal transfection efficiency in MC3T3-E1, C3H10T1/2, HeLa, C2C12, Hep G2, and HCT116 was seen using FuGENE HD, in PT-30, 4T1, and HEKn was seen using Arrest-In, and in MCF-7 was seen using jetPEI. Determination of cytotoxicity showed that the largest amount of viable cells was found after transfection with jetPEI and ExpressFect. These results suggest that FuGENE HD is the most preferred transfection reagent for many cell lines, followed by Arrest-In and jetPEI. These results may be useful for improving nonviral gene and cell therapy applications.

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Section: Discussionsupporting

confidence: 53%

Comparison of Transfection Efficiency of Nonviral Gene Transfer Reagents

2010

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“…Elevated levels of versican have been reported in most malignancies, including brain tumors, melanomas, osteosarcomas, lymphomas and cancer of the breast, prostate, colon, lung, pancreas, endometrium, and ovary [46–48,75,86–98]. Versican expression is also associated with cancer relapse and poor patient outcome in breast, prostate and many other cancer types including ovarian cancer [80].…”

Section: Role Of the Extracellular Matrix In Ovarian Cancer Metastasismentioning

confidence: 99%

“…When polyethylenimine (PEI) DNA particles are conjugated with HA they showed more specific targeting of breast cancer cells with high CD44 expression [98]. A siRNA/PEI-HA complex exhibited higher CD44 gene silencing efficiency in melanoma cells compared to siRNA/PEI complex alone [99] Similarly, liposomes-protamine-HA nanoparticles used for systemic delivery of siRNA into melanoma cells had a broader effective therapeutic dose range than nanoparticles without HA [100].…”

Section: Cancer Therapies Targeting Extracellular Componentsmentioning

confidence: 99%

Role of Versican, Hyaluronan and CD44 in Ovarian Cancer Metastasis

Ween

Oehler

Ricciardelli

2011

IJMS

113

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There is increasing evidence to suggest that extracellular matrix (ECM) components play an active role in tumor progression and are an important determinant for the growth and progression of solid tumors. Tumor cells interfere with the normal programming of ECM biosynthesis and can extensively modify the structure and composition of the matrix. In ovarian cancer alterations in the extracellular environment are critical for tumor initiation and progression and intra-peritoneal dissemination. ECM molecules including versican and hyaluronan (HA) which interacts with the HA receptor, CD44, have been shown to play critical roles in ovarian cancer metastasis. This review focuses on versican, HA, and CD44 and their potential as therapeutic targets for ovarian cancer.

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“…The high viscosity, elasticity, negative charge and biocompatibility of HA have extended its application in biomedical and cosmetic fields. For instance, HA has been used to reduce cytotoxicity in non-viral gene transfer systems (Bahadur, Thapa, & Xu, 2012; Chen et al, 2012; Fan et al, 2013; Feng et al, 2014; He et al, 2013; Sun et al, 2009; Wang et al, 2011; Xu, Quick, & Yeo, 2009). Other HA-based carriers for protein/peptides, drugs and metallic particles have also been investigated (Kwag, Oh, & Lee, 2014; Lee et al, 2012; Li, Yu, Jin, & Yin, 2012; Varshosaz, Ghalaei, & Hassanzadeh, 2014; Xiong et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Investigating triazine-based modification of hyaluronan using statistical designs

Liang

Cheng

Struckhoff

et al. 2015

Carbohydrate Polymers

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Hyaluronan (HA) and its derivatives have been extensively researched for many biomedical applications. To precisely tailor the property of HA by derivatizing it to a pre-determined extent is challenging, yet critical. In this paper, we used 2-chloro-4,6-dimethoxy-1,3,5-triazine (CDMT) and N-methylmorpholine (NMM) to derivatize HA via a triazine-based coupling reaction. Using a fractional factorial (FF) design, we observed that water content in the solvent, and molar ratios of CDMT and NaHCO3 to the carboxylate were the significant factors controlling the derivatization. We investigated how the effect of each factor changes as reaction conditions change. Moreover, by altering the amount of CDMT and NaHCO3, we developed a cubic regression model for precise control of the extent of derivatization using a response surface methodology (RSM) with a D-optimal design. No spurious peaks were detected by 1H NMR spectrum and only 10% decrease of molecular weight of the derivatized HA was determined by GPC. The HA with 6% modification was relatively biocompatible up to 15 mg/mL.

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Positive hyaluronan/PEI/DNA complexes as a target-specific intracellular delivery to malignant breast cancer

Cited by 19 publications

References 24 publications

Comparison of Transfection Efficiency of Nonviral Gene Transfer Reagents

Comparison of Transfection Efficiency of Nonviral Gene Transfer Reagents

Role of Versican, Hyaluronan and CD44 in Ovarian Cancer Metastasis

Investigating triazine-based modification of hyaluronan using statistical designs

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