Positive Surface Charge Enhances Selective Cellular Uptake and Anticancer Efficacy of Selenium Nanoparticles

Yu, Bo; Zhang, Yibo; Zheng, Wenjie; Fan, Cundong; Chen, Tianfeng

doi:10.1021/ic301050v

Cited by 250 publications

(156 citation statements)

References 41 publications

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“…The resultant nanoparticles had a positive zeta potential (19.8 mV), which could be attributed to the octadecylamine with an added ammonium group [35] . It has been demonstrated that nanoparticles with a positive charge are [36] . Additionally, the Malvern Nano-ZS90 laser particle size results showed that the Bre-NLC had a narrow particle dispersion of ~170 nm.…”

Section: Discussionmentioning

confidence: 99%

Development of ionic-complex-based nanostructured lipid carriers to improve the pharmacokinetic profiles of breviscapine

Zheng

Shan

et al. 2013

Acta Pharmacol Sin

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Aim: Breviscapine isolated from the Chinese herb Erigeron breviscapus (Vant) Hand-Mazz is widely used to treat cardiovascular and cerebrovascular diseases. The aim of this study was to improve the pharmacokinetic profiles of breviscapine using nanostructured lipid carrier based on an ionic complex formation. Methods: Breviscapine nanostructured lipid carrier (Bre-NLC) was prepared using the thin film homogenization method. The morphology of Bre-NLCs was determined using transmission electron microscopy. The mean particle size, polydispersity index, zeta-potential analysis and entrapment efficiency were analized. In vitro release was studied using the dialysis method. In vitro stability was studied in fresh plasma and liver slurry of rats. In vivo pharmacokinetics was analyzed in rats after intravenous injection of a dose equivalent to breviscapine (10 mg/kg). Results: The Bre-NLCs were spherical with a mean particle size of ~170 nm, a zeta potential of ~20 mV and a high entrapment efficiency of ~89%. Compared with a commercially available solution, a substantial decrease in the cumulative release of breviscapine was found for the Bre-NLCs. The NLC has a significantly protective effect against the liver enzyme degradation of breviscapine. After intravenous administration in rats, the Bre-NLCs exhibited a 32 times increase in the AUC 0-t and a 12 times increase in T 1/2 as compared to the commercially available breviscapine solution. Conclusion:The results demonstrate that the NLC has great potential to use as a novel sustained release system for breviscapine.

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Section: Discussionmentioning

confidence: 99%

Development of ionic-complex-based nanostructured lipid carriers to improve the pharmacokinetic profiles of breviscapine

Zheng

Shan

et al. 2013

Acta Pharmacol Sin

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“…46,47 However, there are very few reports on the anticancer activity of SeNPs synthesized by bacteria. 37,48 SeNPs synthesized by Bacillus strain ZYK have profound anticancer activity against H157 lung cancer cell line.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Green synthesis of selenium nanoparticles using<em> Acinetobacter</em> sp. SW30: optimization, characterization and its anticancer activity in breast cancer cells

Wadhwani

Gorain

Banerjee

et al. 2017

IJN

156

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The aim of this study was to synthesize selenium nanoparticles (SeNPs) using cell suspension and total cell protein of Acinetobacter sp. SW30 and optimize its synthesis by studying the influence of physiological and physicochemical parameters. Also, we aimed to compare its anticancer activity with that of chemically synthesized SeNPs in breast cancer cells. Cell suspension of Acinetobacter sp. SW30 was exposed to various physiological and physicochemical conditions in the presence of sodium selenite to study their effects on the synthesis and morphology of SeNPs. Breast cancer cells (4T1, MCF-7) and noncancer cells (NIH/3T3, HEK293) were exposed to different concentrations of SeNPs. The 18 h grown culture with 2.7×10 9 cfu/mL could synthesize amorphous nanospheres of size 78 nm at 1.5 mM and crystalline nanorods at above 2.0 mM Na 2 SeO 3 concentration. Polygonal-shaped SeNPs of average size 79 nm were obtained in the supernatant of 4 mg/mL of total cell protein of Acinetobacter sp. SW30. Chemical SeNPs showed more anticancer activity than SeNPs synthesized by Acinetobacter sp. SW30 (BSeNPs), but they were found to be toxic to noncancer cells also. However, BSeNPs were selective against breast cancer cells than chemical ones. Results suggest that BSeNPs are a good choice of selection as anticancer agents.

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“…38 Earlier, we demonstrated that the zeta potential of MLP increased significantly under hypoxic and low-pH conditions. Whether MLP could enhance the cellular uptake under hypoxic and low-pH conditions, and the cellular uptake and intracellular distribution of the MLP/siRNA were studied by fluorescence microscopy and flow cytometry.…”

Section: Intracellular Uptake Of Mlp/sirna By C6 Cellsmentioning

confidence: 86%

Hypoxia-responsive ionizable liposome delivery siRNA for glioma therapy

Liu¹,

Zhang²,

Xie³

et al. 2017

IJN

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Here, we report the hypoxia-responsive ionizable liposomes to deliver small interference RNA (siRNA) anticancer drugs, which can selectively enhance cellular uptake of the siRNA under hypoxic and low-pH conditions to cure glioma. For this purpose, malate dehydrogenase lipid molecules were synthesized, which contain nitroimidazole groups that impart hypoxia sensitivity and specificity as hydrophobic tails, and tertiary amines as hydrophilic head groups. These malate dehydrogenase molecules, together with DSPE-PEG2000 and cholesterol, were self-assembled into O′ 1 ,O 1 -(3-(dimethylamino)propane-1,2-diyl) 16-bis(2-(2-methyl-5-nitro-1 H -imidazol-1-yl)ethyl) di(hexadecanedioate) liposomes (MLP) to encapsulate siRNA through electrostatic interaction. Our study showed that the MLP could deliver polo-like kinase 1 siRNA (siPLK1) into glioma cells and effectively enhance the cellular uptake of MLP/siPLK1 because of increased positive charges induced by hypoxia and low pH. Moreover, MLP/siPLK1 was shown to be very effective in inhibiting the growth of glioma cells both in vitro and in vivo. Therefore, the MLP is a promising siRNA delivery system for tumor therapy.

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Positive Surface Charge Enhances Selective Cellular Uptake and Anticancer Efficacy of Selenium Nanoparticles

Cited by 250 publications

References 41 publications

Development of ionic-complex-based nanostructured lipid carriers to improve the pharmacokinetic profiles of breviscapine

Development of ionic-complex-based nanostructured lipid carriers to improve the pharmacokinetic profiles of breviscapine

Green synthesis of selenium nanoparticles using<em> Acinetobacter</em> sp. SW30: optimization, characterization and its anticancer activity in breast cancer cells

Hypoxia-responsive ionizable liposome delivery siRNA for glioma therapy

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