Possible direct induction by estrogen of calcitonin secretion from ultimobranchial cells in the goldfish

Suzuki, Nobuo; Yamamoto, Kazutoshi; Sasayama, Yuichi; Suzuki, Tohru; Kurokawa, Tadahide; Kambegawa, Akira; Srivastav, Ajai K.; Hayashi, Shinji; Kikuyama, Sakaé

doi:10.1016/j.ygcen.2004.05.013

Cited by 26 publications

(34 citation statements)

References 40 publications

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“…In fact, the plasma calcitonin level of female teleost increases in the reproductive period [38,41]. Using an in vitro assay system, we found that goldfish calcitonin suppressed osteoclastic activity in the goldfish scales [17].…”

Section: Discussionmentioning

confidence: 87%

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

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The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100 pg/ml to 10 ng/ml) significantly increased ALP activity at 6 h of incubation. High-dose (10 ng/ml) fugu PTH1 significantly increased ALP activity even after 18 h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6 h of incubation, but fugu PTH1 (100 pg/ml to 10 ng/ml) significantly increased TRAP activity at 18 h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-B ligand in osteoblasts and the receptor activator NF-B mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.3

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Section: Discussionmentioning

confidence: 87%

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Suzuki

Danks

Maruyama

et al. 2011

Bone

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“…The experiments were performed three times in duplicate, and the values are (Deftos et al 1974, Watts et al 1975, Yamauchi et al 1976, 1978, Bjorsson et al 1986, Norberg et al 1989. The E 2 increases plasma CT levels in rainbow trout (Bjornsson et al 1986(Bjornsson et al , 1989) and a direct induction of estrogen on CT secretion from ultimobranchial glands in goldfish has also been suggested (Suzuki et al 2004). Although a possible explanation for the hyperactivity of ultimobranchial gland and the rise in plasma CT levels during peak reproductive season in female fish has been put forwarded by many workers (Bjornsson et al 1989, Brown & Bern 1989, Suzuki et al 2004), none of them were able to suggest an exact relationship between CT and reproduction in fish.…”

Section: Discussionmentioning

confidence: 99%

“…Available information suggests that CT is involved in mobilizing calcium or in directing its mobilization by protection of a certain calcium pool during vitellogenesis (Bjorsson et al 1989). Suzuki et al (2004) described that E 2 acts on UBG to induce the release of CT, which in turn may play an important role in reproduction directly and or indirectly through calcium. Although these studies indicate a relationship between CT and fish reproduction they fail to explain the exact link between them.…”

Section: Introductionmentioning

confidence: 99%

Stimulation of salmon calcitonin on secretion of 17β-estradiol by the ovarian follicles of common carp, Cyprinus carpio

Paul

Mukherjee²,

Pramanick³

et al. 2007

Journal of Endocrinology

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The effects of salmon calcitonin (sCT) on the secretion of 17b-estradiol (E 2 ) were examined in female common carp, Cyprinus carpio. Vitellogenic stage fish adapted to high-Ca water were i.p. injected with vehicle, sCT, human chorionic gonadotropin (hCG), or hCG plus sCT. To determine whether ovarian follicles are equipped with CT receptors, a CT binding assay was conducted. In the in vitro experiments, vitellogenic follicles were incubated with stimulators and inhibitors. Administration of sCT increased the basal and hCG-stimulated E 2 release in vivo and in vitro. Binding characteristics of [ 125 I]sCT to plasma membrane preparation of carp ovarian follicles showed saturability with high-affinity (K d Z48 . 48 pmol/l and B max Z1 . 2 pmol/mg protein). To clarify the mechanism of E 2 production by sCT, in vitro effect of sCT and hCG on aromatase activity (conversion of testosterone to E 2 ) and cytochrome P450 aromatase (P450arom) gene expression in carp ovarian follicles were investigated. Salmon CT-stimulated both aromatase activity and P450arom gene expression in ovarian follicles of carp. sCT-stimulated E 2 release by the ovarian follicles in vitro was augmented in the presence of dibutyryl cAMP. Inhibitor of protein kinase A (PKA), SQ 22536 inhibited sCT-stimulated steroid production in a dose-dependent manner. Specific inhibitor of protein kinase C (PKC), NPC-15437 dihydrochloride had no inhibitory effects on sCT-induced E 2 release. The present study indicates that sCT binds specifically to carp ovary and stimulates E 2 production by increasing the activity of cytochrome P450 aromatase and P450arom gene expression. The results further suggest that stimulatory action of sCTon E 2 production is mediated through cAMP pathway.

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“…17β-estradiol (E 2 ) increases plasma CT levels in rainbow trout (Bjorsson et al, 1986(Bjorsson et al, , 1989) and a direct induction of estrogen on CT secretion from ultimobranchial glands in goldfish has also been suggested (Suzuki et al, 2004). Possible explanation for the hyperactivity of ultimobranchial gland and the rise in plasma CT levels during peak reproductive season in female fish has been put forwarded by many workers (Brown and Bern 1989;Bjorsson et al, 1989;Suzuki et al, 2004), but none of them were able to suggest an exact relationship between CT and reproduction in fish. The present study provide evidence that sCT is effective in increasing plasma E 2 levels in vitellogenic stage fish and stimulating both spontaneous and hCG-induced secretion of E 2 by the ovarian follicles in vitro.…”

Section: Discussionmentioning

confidence: 99%

Calcitonin Functions Both as a Hypocalcemic Hormone and Stimulator of Steroid Production and Oocyte Maturation in Ovarian Follicles of Common Carp, Cyprinus carpio

Mukherjee¹,

Kundu²,

Pramanick³

et al. 2011

Update on Mechanisms of Hormone Action - Focus on Metabolism, Growth and Reproduction

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Possible direct induction by estrogen of calcitonin secretion from ultimobranchial cells in the goldfish

Cited by 26 publications

References 40 publications

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Parathyroid hormone 1 (1–34) acts on the scales and involves calcium metabolism in goldfish

Stimulation of salmon calcitonin on secretion of 17β-estradiol by the ovarian follicles of common carp, Cyprinus carpio

Calcitonin Functions Both as a Hypocalcemic Hormone and Stimulator of Steroid Production and Oocyte Maturation in Ovarian Follicles of Common Carp, Cyprinus carpio

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