Post-harvest fungal pathogens are reported to cause 10 to 25 percent losses in apple. Among various pathogens, white rot caused by Botryosphaeria dothidea is one of the important post-harvest diseases. Incidence of white rot was recorded between3.0-14.1 percent in important marketing yards of Himachal Pradesh. Golden Delicious variety of apple has been found to be most susceptible to white rot pathogen. Two botanical formulations by mixing equal quantity of leaves of Karu (Roylea elegans), Artemisia (Artemisia roxburghiana), Neem (Azadirachta indica), Bana (Vitex negundo), Tulsi (Ocimum sanctum) and seeds of Darek (Melia azedarach) made in water and cowurine were evaluated against the white rot pathogen. Fruit dip for 30 minutes in cow urine based formulation did not allowed the disease development to form lesions in artificially inoculated apple fruits. Also, fruits dipped in cow urine based formulation followed by their storage in card board boxes at room temperature (25± 3 0 C) for 30 days storage had minimum incidence (2.8%) of white rot disease. Therefore, dip treatment of apple fruits with different bio -resources including water and cow urine based botanical formulation separately proved effective in the management of white rot (Botryosphaeria dothidea) of apple.