Post-mortem changes in metabolomic profiles of human serum, aqueous humor and vitreous humor

Zelentsova, Ekaterina A.; Yanshole, Lyudmila V.; Melnikov, Arsenty D; Kudryavtsev, Ivan S; Новоселов, В. П.; Tsentalovich, Yuri P.

doi:10.1007/s11306-020-01700-3

Cited by 40 publications

(33 citation statements)

References 42 publications

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“…Potassium showed the best correlation between the two biofluids, regardless of early or late PMIs and adult or paediatric population. Very recently a metabolomic approach on human AH, VH and serum confirmed that AH is a viable biofluid for the estimation of PMI [21].…”

Section: Introductionmentioning

confidence: 97%

Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

et al. 2020

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Estimation of the post-mortem interval (PMI) remains a matter of concern in the forensic scenario. Traditional and novel approaches are not yet able to fully address this issue, which relies on complex biological phenomena triggered by death. For this purpose, eye compartments may be chosen for experimental studies because they are more resistant to post-mortem modifications. Vitreous humour, in particular, has been extensively investigated, with potassium concentration ([K+]) being the marker that is better correlated with PMI estimation. Recently, a 1H nuclear magnetic resonance (NMR) metabolomic approach based on aqueous humour (AH) from an animal model was proposed for PMI estimation, resulting in a robust and validated regression model. Here we studied the variation in [K+] in the same experimental setup. [K+] was determined through capillary ion analysis (CIA) and a regression analysis was performed. Moreover, it was investigated whether the PMI information related to potassium could improve the metabolome predictive power in estimating the PMI. Interestingly, we found that a part of the metabolomic profile is able to explain most of the information carried by potassium, suggesting that the rise in both potassium and metabolite concentrations relies on a similar biological mechanism. In the first 24-h PMI window, the AH metabolomic profile shows greater predictive power than [K+] behaviour, suggesting its potential use as an additional tool for estimating the time since death.

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Section: Introductionmentioning

confidence: 97%

Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

et al. 2020

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“…We also observed a profound increase in the concentrations of xanthine, hypoxanthine, β-alanine, and β-aminoisobutyrate (Figure 4, Table 2), which are nucleotide degradation products and are often observed in decaying tissues [55][56][57][58]. This indicates that the stress is so dramatic that it results in nucleotide degradation.…”

Section: Markers Of Stressmentioning

confidence: 88%

Metabolomic Analysis Reveals That the Moor Frog Rana arvalis Uses Both Glucose and Glycerol as Cryoprotectants

Shekhovtsov

Булахова

Tsentalovich

et al. 2022

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The moor frog Rana arvalis is one of a few amphibians that can tolerate freezing to low temperatures, up to −16 °C. In this study, we performed metabolomic analysis of the liver and hindlimb muscles of frozen and control R. arvalis. We found that the moor frog synthesizes glucose and glycerol in similar concentrations as low molecular weight cryoprotectants. This is the first such case reported for the genus Rana, which was believed to use glucose only. We found that freezing upregulates glycolysis, with the accumulation of several end products: lactate, alanine, ethanol, and, possibly, 2,3-butanediol. To our knowledge, this is also the first report of ethanol as an end product of glycolysis in terrestrial vertebrates. We observed highly increased concentrations of nucleotide degradation products, implying high level of stress. The Krebs cycle arrest resulted in high concentrations of succinate, which is common for animals. However, we found almost no signs of adaptations to reoxygenation stress, with overall low levels of antioxidants. We also performed metabolomics analysis of subcutaneous ice that was found to contain glucose, glycerol, and several other substances.

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“…From a metabolic point of view, the absence of nutrients from the blood and vitreous associated with hypoxia leads to alterations in the corneal tissues and the level of the aqueous and vitreous humor [ 31 , 32 , 33 ].…”

Section: Discussionmentioning

confidence: 99%

The Influence of Eyelid Position and Environmental Conditions on the Corneal Changes in Early Postmortem Interval: A Prospective, Multicentric OCT Study

et al. 2022

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In the current study, using portable optical coherence tomography, we evaluated 46 corneas of 23 individuals in a multicenter setting during the first 17 h after death. Twenty-three eyes were kept open, and twenty three were kept closed. Furthermore, the experiment was carried out for 12 samples in summer and 11 in winter. Our data show that postmortem corneal alterations largely depend on the phenomena of dehydration (in particular in open eyes) and swelling of the stroma in closed eyes, probably due in the first phase to hypoxia/anoxia and subsequently to the passage by osmosis of the aqueous humor from the anterior chamber to the corneal tissue. Our findings could have significant repercussions in forensic pathology for estimating the postmortem interval and transplantation to optimize the conservation of the tissue before the explant.

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Post-mortem changes in metabolomic profiles of human serum, aqueous humor and vitreous humor

Cited by 40 publications

References 42 publications

Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

Comparative use of aqueous humour 1H NMR metabolomics and potassium concentration for PMI estimation in an animal model

Metabolomic Analysis Reveals That the Moor Frog Rana arvalis Uses Both Glucose and Glycerol as Cryoprotectants

The Influence of Eyelid Position and Environmental Conditions on the Corneal Changes in Early Postmortem Interval: A Prospective, Multicentric OCT Study

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