Post-trauma behavioral phenotype predicts the degree of vulnerability to fear relapse after extinction in male rats

Demars, Fanny; Todorova, Ralitsa; Makdah, Gabriel; Forestier, Antonin; Krebs, Marie‐Odile; Godsil, Bill P.; Jay, Thérèse M.; Wiener, Sidney I.; Pompili, Marco N.

doi:10.1016/j.cub.2022.05.050

Cited by 10 publications

(3 citation statements)

References 71 publications

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“…However, the change from jumping to darting midway through the first extinction session in the PA group suggests that the darting observed in these mice is not the result of sensitization. Indeed, darting behavior has been shown to change based on multiple parameters, decreasing both with increased shock intensity (Mitchel et al, 2023) and with prolonged extinction training (Demars et al, 2022), suggesting that darting is both an associative conditioned response to stimuli with intermediate threat value and a defensive response resulting from sensitization.…”

Section: Discussionmentioning

confidence: 99%

Contributions of associative and non-associative learning to the dynamics of defensive ethograms

Le,

Hereford,

Borkar

et al. 2023

Preprint

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Defensive behavior changes based on threat intensity, proximity, and context of exposure, and learning about danger-predicting stimuli is critical for survival. However, the contributions of associative and non-associative mechanisms to dynamic defensive responses are currently unclear given the reductionist behavior elicited by most Pavlovian fear conditioning paradigms. To investigate defensive ethograms more thoroughly, we subjected male and female adult C57BL/6J mice to a Pavlovian threat conditioning paradigm that pairs footshock with a serial compound stimulus (SCS) consisting of distinct tone and white noise (WN) stimulus periods. To investigate how associative and non-associative mechanisms affect defensive responses, we compared the paired SCS-footshock group with control groups that either received randomly presented SCS and footshock presentations (unpaired) or received only footshocks during conditioning (shock-only). After conditioning, only the paired group exhibited robust freezing during the tone period with switching to explosive flight responses comprised of jumping and darting behavior during the WN period. The unpaired group expressed no cue-induced freezing and significantly less freezing overall. Furthermore, the unpaired group reduced freezing but rarely showed jumping or darting during WN. Following conditioning, we observed how defensive behavior changed over two extinction sessions. During extinction, only the paired group decreased tone-induced freezing. During WN, the paired group rapidly transitioned from escape jumping to freezing and darting. Comparatively, the unpaired and shock-only groups displayed stress-induced tail rattling to SCS, eschewing freezing and jumping. These findings demonstrate that while non-associative factors promote some defensive responsiveness, associative pairings are required for robust cue-induced freezing and flight expression.

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Section: Discussionmentioning

confidence: 99%

Contributions of associative and non-associative learning to the dynamics of defensive ethograms

Le,

Hereford,

Borkar

et al. 2023

Preprint

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“…Beyond neurophysiology, it is also useful to reduce high dimensional behavioral datasets (e.g. [27]). It reduces the original dataset into a set of dimensions (or factors) defined by orthogonal principal components (or eigenvectors, the cell assemblies), each resulting from the linear combination of the original variables (i.e., neurons) [28, 29].…”

Section: Detecting Cell Assemblies With Dimensionality Reductionmentioning

confidence: 99%

Detection of Cell Assemblies in High-Density Extracellular Electrophysiological Recordings

Makdah,

Wiener,

Pompili

2024

Preprint

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Cell assemblies, i.e., concurrently active groups of neurons, likely underlie neural processing for higher brain functions. Recent technological progress has enabled large-scale recording of neuronal activity, permitting the exploration and analysis of cell assembly dynamics. This review aims to provide both conceptual insights and practical knowledge pertaining to principal methodologies used for detecting cell assemblies in the last fifteen years. The goal is to assist readers in selecting and comparing various protocols to optimize their data processing and analysis pipeline. Each algorithm is explained with its fundamental principles, their application in neuroscience for cell assembly detection, and illustrated with published studies. Recognizing the similarities, advantages, and drawbacks of diverse methodologies may pave the way for developing new procedures for cell assembly identification to facilitate future endeavors in the understanding of brain activity.

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“…However, there are fewer examples of chronic recording in unrestrained animals (Krupic et al, 2018;Gardner et al, 2019;Böhm and Lee, 2020). Yet to understand the neural mechanisms underlying cognition it is essential to track behavior and neuronal activity in longitudinal studies in freely moving animals (Pompili and Todorova, 2022), ideally in ethologically relevant settings (Demars et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Long-term near-continuous recording with neuropixels probes in healthy and epileptic rats

Ghestem

Pompili

Dipper-Wawra

et al. 2023

Preprint

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Neuropixels probes have become a crucial tool for high-density electrophysiological recordings. Although most research involving these electrodes is in acute preparations, some scientific inquiries require long-term recordings in freely moving animals. Recent reports have presented prosthesis designs for chronic recordings, but some of them do not allow for probe recovery, which is desirable given their cost. Others appear to be fragile, as these articles describe numerous broken implants. This fragility presents a challenge for recordings in rats, particularly in epilepsy models where strong mechanical stress impinges upon the prosthesis. To overcome these limitations, we present a new implant specifically designed to protect the probes from strong shocks and enable the safe retrieval of electrodes after experiments. This implant was successfully used to record from healthy and epileptic rats for up to three weeks almost continuously, with a probe retrieval and reuse success rate of 91%, improving previously described recycling performances.

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Post-trauma behavioral phenotype predicts the degree of vulnerability to fear relapse after extinction in male rats

Cited by 10 publications

References 71 publications

Contributions of associative and non-associative learning to the dynamics of defensive ethograms

Contributions of associative and non-associative learning to the dynamics of defensive ethograms

Detection of Cell Assemblies in High-Density Extracellular Electrophysiological Recordings

Long-term near-continuous recording with neuropixels probes in healthy and epileptic rats

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